A5063760497- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Ferroptosis and cancer prognosis
- Cell Image Analysis Techniques
- Barrier Structure and Function Studies
- Alzheimer's disease research and treatments
- Drug Transport and Resistance Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- 3D Printing in Biomedical Research
- Immune Response and Inflammation
- MRI in cancer diagnosis
- Bone and Joint Diseases
- Anesthesia and Neurotoxicity Research
University of Auckland
2019-2025
Abstract Background Microglia and tumor-associated macrophages (TAMs) constitute up to half of the total tumor mass glioblastomas. Despite these myeloid populations being ontogenetically distinct, they have been largely conflated. Recent single-cell transcriptomic studies identified genes that distinguish microglia from TAMs. Here we investigated whether translated proteins enriched in microglial or TAM can be used differentiate cells immunohistochemically stained human glioblastoma tissue....
Abstract Parkinson’s disease (PD) is characterised by the progressive loss of midbrain dopaminergic neurons and presence aggregated α-synuclein (α-syn). Pericytes microglia, two non-neuronal cells contain α-syn in human brain, however, their role processes poorly understood. Pericytes, found surrounding capillaries brain are important for maintaining blood–brain barrier, controlling blood flow mediating inflammation. In this study, primary pericytes microglia were exposed to different...
Microglia perform key homeostatic functions to protect the central nervous system (CNS). However, in many brain disorders their protective are abrogated, contributing disease progression. Therefore, studies of microglial function critical developing treatments for disorders. Different vitro microglia models have been established, including primary human and rodent cells, induced pluripotent stem cell (iPSC)-derived models, immortalised lines. a direct comparative analysis phenotypic...
Microglia are the primary innate immune effectors of central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, isolation adult microglia has proven more difficult. Here, we present a simple, widely accessible protocol pure cultures from 4- 14-month-old brains using their adherent properties in vitro. These isolated recapitulate human and suitable model for studying age-related diseases. For complete details on use execution this please refer...
Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. Whilst role of efflux transporters are well established GBM, expression function uptake transporters, such as organic anion transporting polypeptide (OATP) family, not understood. OATPs possess broad substrate specificity that includes anti-cancer agents; therefore, we sought to investigate four OATP isoforms human GBM cell types using patient tissue.We used fluorescent immunohistochemical labeling...
Abstract BACKGROUND Glioblastoma tumours are highly vascular and angiogenic. However, they also heterogeneous, comprising many different macro- microenvironments. Reflecting this, tumour-associated blood vessels variable in their distribution, structure, function. Despite the significance of vasculature development progression glioblastoma, its complexity remains poorly defined. Therefore, characterising diversity across glioblastoma landscape is a crucial step targeted therapies. METHODS 40...
Abstract BACKGROUND Glioblastoma multiforme (GBM) is the most common primary brain tumour that affects adults. This aggressive invariably fatal, carrying a rapid progression and dismal median survival period of only 15 months despite multimodal treatment approaches. Central to GBM pathogenesis immunosuppressive profile these tumours. The two cell types are highly abundant in tumours play critical roles niche brain’s resident microglia their peripheral counterparts - associated macrophages...
Abstract Background Microglia and bone marrow-derived macrophages (BMDMs) are two ontogenetically distinct myeloid populations present within glioblastoma that can comprise 30-50% of the tumour mass. Historically, these cell types have been conflated studied as a single population ‘tumour-associated macrophages’. Recent advances in single-cell omics allowed delineation, suggesting microglia BMDMs may play different roles subsequently differentially affect progression. Despite building...