A5060558154- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
- Cell Image Analysis Techniques
- Neurobiology and Insect Physiology Research
- Retinal Diseases and Treatments
- Advanced Biosensing Techniques and Applications
- Molecular Biology Techniques and Applications
- Glaucoma and retinal disorders
- Marine animal studies overview
- Parkinson's Disease Mechanisms and Treatments
- Plant-Microbe Interactions and Immunity
- Retinal and Macular Surgery
- Ubiquitin and proteasome pathways
- AI in cancer detection
- Advanced biosensing and bioanalysis techniques
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Retinal Development and Disorders
- Intraocular Surgery and Lenses
Stockholm University
2023-2025
Science for Life Laboratory
2023-2025
Centre National de la Recherche Scientifique
2014-2024
University of Catania
2020-2024
Université Paris Sciences et Lettres
2024
Institut Curie
2023-2024
Sorbonne Université
2024
Laboratoire de Recherche Scientifique
2020-2021
Institut de Génomique Fonctionnelle de Lyon
2016-2021
École Normale Supérieure de Lyon
2016-2021
The amniote pallium contains sensory circuits that are structurally and functionally equivalent, yet their evolutionary relationship remains unresolved. We used birthdating analysis, single-cell RNA spatial transcriptomics, mathematical modeling to compare the development evolution of known pallial across birds (chick), lizards (gecko), mammals (mouse). reveal neurons within these circuits’ stations generated at varying developmental times brain regions species found an early divergence in...
The amphipod crustacean Parhyale hawaiensis is a blossoming model system for studies of developmental mechanisms and more recently regeneration. We have sequenced the genome allowing annotation all key signaling pathways, transcription factors, non-coding RNAs that will enhance ongoing functional studies. member Malacostraca clade, which includes food crop species. analysed immunity related genes as an important comparative these species, where aquaculture problems increased farming has...
Cell lineages provide the framework for understanding how cell fates are decided during development. Describing in most organisms is challenging; even a fruit fly larva has ~50,000 cells and small mammal >1 billion cells. Recently, idea of applying CRISPR to induce mutations development, be used as heritable markers lineage reconstruction, been proposed by several groups. While an attractive idea, its practical value depends on accuracy that can generated. Here, we use computer simulations...
Abstract The Xenium In Situ platform is a new spatial transcriptomics product commercialized by 10x Genomics, capable of mapping hundreds genes in situ at subcellular resolution. Given the multitude commercially available technologies, recommendations choice and analysis guidelines are increasingly important. Herein, we explore 25 datasets generated from multiple tissues species, comparing scalability, resolution, data quality, capacities limitations with eight other spatially resolved...
A thinning of intraretinal layers has been previously described in Parkinson's disease (PD) patients compared to healthy controls (HCs). Few studies evaluated the possible correlation between retinal thickness and microvascularization. Thus, here we assessed microvascular pattern early PD HCs, using, respectively, spectral-domain optical coherence tomography (SD-OCT) SD-OCT-angiography (SD-OCT-A), more interestingly, a pattern. Patients fulfilling criteria for clinically...
Abstract Background Glioblastoma (GB), particularly IDH-wildtype, is the most aggressive brain malignancy with a dismal prognosis. Despite advances in molecular profiling, complexity of its tumor microenvironment and spatial organization remains poorly understood. This study aimed to create comprehensive single-cell atlas GB unravel cellular heterogeneity, architecture, clinical relevance. Methods We integrated RNA sequencing data from 26 datasets, encompassing over 1.1 million cells 240...
The repeated use of signalling pathways is a common phenomenon but little known about how they become co-opted in different contexts. Here we examined this issue by analysing the activation Drosophila Torso receptor embryogenesis and pupariation. While its putative ligand differs each case, show that Torso-like, not other proteins required for embryogenesis, also In addition, demonstrate distinct enhancers control torso-like expression both scenarios. We conclude linked to duplication...
Objectives To assess the reversibility of retinal microvascular changes in long term and to investigate potential links with other vascular diseases COVID-19. Methods We designed a prospective multicenter observational study. Patients were enrolled from Methuselah study cohort. Retinal function was studied these patients using optical coherence tomography angiography (OCTA); aortic stiffness measured pulse wave velocity. These examinations performed 1 (Visit 1) 12 2) months after hospital...
Multiplexed spatial profiling of mRNAs has recently gained traction as a tool to explore the cellular diversity and architecture tissues. We propose sensitive, open-source, simple flexible method for generation in-situ expression maps hundreds genes. exploit direct ligation padlock probes on mRNAs, coupled with rolling circle amplification hybridization-based in situ combinatorial barcoding, achieve high detection efficiency, throughput large multiplexing. validate across number species,...
Abstract The amniote pallium contains sensory circuits structurally and functionally equivalent, yet their evolutionary relationship remains unresolved. Our study employs birthdating analysis, single-cell RNA spatial transcriptomics, mathematical modeling to compare the development evolution of known pallial across birds (chick), lizards (gecko) mammals (mouse). We reveal that neurons within these circuits’ stations are generated at varying developmental times brain regions species, found an...
Embryonic development is a complex and dynamic process that unfolds over time involves the production diversification of increasing numbers cells. The impact developmental on formation central nervous system well documented, with evidence showing plays crucial role in establishing identity neuronal subtypes. However, study how translates into genetic instructions driving cell fate limited by scarcity suitable experimental tools. We introduce BirthSeq, new method for isolating analyzing cells...
Abstract Recent innovations in genetics and imaging are providing the means to reconstruct cell lineages, either by tracking divisions using live microscopy, or deducing history of cells molecular recorders. A lineage on its own, however, is simply a description as branching events. major goal current research integrate this relationships with information about spatial distribution identities those produce. Visualizing, interpreting exploring these complex data an intuitive manner requires...
ABSTRACT Multiplexed spatial profiling of mRNAs has recently gained traction as a tool to explore the cellular diversity and architecture tissues. We propose sensitive, open-source, simple flexible method for generation in situ expression maps hundreds genes. use direct ligation padlock probes on mRNAs, coupled with rolling circle amplification hybridization-based combinatorial barcoding, achieve high detection efficiency, high-throughput large multiplexing. validate across number species...
Abstract Using a differential display (DDRT‐PCR) approach to screen genes involved in response osmotic stress, we isolated six cDNA clones from potato cell cultures. One of these, clone #54, is member the gene family encoding α‐subunit translation elongation factor 1 (EF‐1α). After sequencing, this showed deduced amino acid sequence 447 residues. Southern blot analysis revealed that EF‐1α consisting at least nine genes. The expression increased upon stress mediated by polyethylene glycol...
ABSTRACT Embryonic development is a complex and dynamic process that unfolds over time involves the production of increasing numbers cells, as well diversification different cell types. The impact developmental on formation central nervous system well-documented, with evidence showing plays critical role in establishing identity neuronal subtypes. However, study how translates into genetic instructions driving fate limited by scarcity suitable experimental tools. We introduce BirthSeq , new...
Early embryogenesis in Drosophila melanogaster is controlled by maternal gene products, which are deposited the egg during oogenesis. It not well understood how expression germline development. pipsqueak (psq) a complex locus that encodes several nuclear protein variants containing PSQ DNA-binding domain and BTB/POZ domain. Psq proteins thought to regulate through epigenetic silencing. While psq was originally identified as posterior-group gene, we show here novel role of embryonic terminal...
Abstract Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that barcodes used in situ genotyping are expressed from chromosome. This has not been possible bacteria. Here we describe a method with genomic Escherichia. coli . The applied measure intracellular maturation time 81 red fluorescent proteins.
SUMMARY Vardenafil is a highly selective phosphodiesterase-5 (PDE-5) inhibitor approved for the treatment of erectile dysfunction. PDE-5 inhibition determines increase in intracellular cGMP corpora cavernosa that, response to sexual stimuli, results enhanced erections. It has been found be effective high percentage patients and broad spectrum underlying conditions. In particular, men suffering from diabetes, hypertension, with cardiovascular risk factors disease, as well spinal cord injury...