A5024722917- RNA and protein synthesis mechanisms
- T-cell and Retrovirus Studies
- Chromosomal and Genetic Variations
- Genetics, Bioinformatics, and Biomedical Research
- Cancer Genomics and Diagnostics
- Protein Structure and Dynamics
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
High Throughput Biology (United States)
2017
Johns Hopkins University
2017
University of California, Berkeley
2017
We describe complete design of a synthetic eukaryotic genome, Sc2.0, highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases the deleted, inserted, or altered. Sc2.0 chromosome was implemented BioStudio, an open-source framework developed for design, which coordinates modifications from nucleotide to scales and enforces version control systematically track edits. To achieve synthesis, individual chromosomes built Consortium teams around world will be...
ABSTRACT Over the past thirty years, site-directed mutagenesis has become established as one of most powerful techniques to probe enzyme reaction mechanisms 1-3 . Substitutions active site residues are likely yield significant perturbations in kinetic parameters, but there many examples profound changes these values elicited by remote mutations 4-6 Ortholog comparisons extant sequences show that do not have influence on function. As number potential single natural amino acid substitutions...