A5027115564- Force Microscopy Techniques and Applications
- Antifungal resistance and susceptibility
- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Fungal Infections and Studies
- Polymer Surface Interaction Studies
- Nanopore and Nanochannel Transport Studies
- Probiotics and Fermented Foods
- Molecular Junctions and Nanostructures
- Mechanical and Optical Resonators
- Nanoparticle-Based Drug Delivery
- Silicon Nanostructures and Photoluminescence
- Clostridium difficile and Clostridium perfringens research
- Computational Drug Discovery Methods
- Botulinum Toxin and Related Neurological Disorders
- Pharmacogenetics and Drug Metabolism
- Advanced Electron Microscopy Techniques and Applications
- Protein Structure and Dynamics
- Biochemical and Structural Characterization
- Advanced Biosensing Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- biodegradable polymer synthesis and properties
- Cancer therapeutics and mechanisms
University of Missouri
2020-2025
Candida albicans causes severe invasive candidiasis. C. infection requires the virulence factor candidalysin (CL) which damages target cell membranes. However, mechanism that CL uses to permeabilize membranes is unclear. We reveal forms membrane pores using a unique mechanism. Unexpectedly, readily assembled into polymers in solution. propose basic structural unit polymer formation oligomer, sequentially added string configuration can close loop. loops appear spontaneously insert become...
Membrane proteins play critical roles in disease and the disposition of many pharmaceuticals. A prime example is P-glycoprotein (Pgp) which moves a diverse range drugs across membranes out cell before therapeutic payload can be delivered. Conventional structural biology methods have provided valuable framework for comprehending complex conformational changes underlying Pgp function, also includes ATPase activity, but lack real-time information hinders understanding. Atomic force microscopy...
P-glycoprotein (Pgp) plays a pivotal role in drug bioavailability and multi-drug resistance development. Understanding the protein's activity designing effective drugs require insight into mechanisms underlying Pgp-mediated transport of xenobiotics. In this study, we investigated drug-induced conformational changes Pgp adopted conformationally-gated model to elucidate camptothecin analogs (CPTs). While displays wide range conformations, simplified it three states: 'open-inward',...
Abstract The fundamental molecules of life are polymers. Prominent examples include nucleic acids and proteins, both which assume a vast array mechanical properties three-dimensional shapes. persistence length represents numerical value to classify the bending rigidity individual shape polymer, dictated by topology polymer backbone - line trace through center along contour path – is also critical metric. Common architectures linear, ring-like or cyclic, branched; combinations these can...
Candidalysin is a cytolytic peptide produced by the opportunistic fungal pathogen
Intrinsic apoptosis is orchestrated by a group of proteins that mediate the coordinated disruption mitochondrial membranes. Bax multi-domain protein that, upon activation, disrupts integrity outer membrane forming pores. We strategically introduced glutamic acids into short sequence constitutively creates The resulting BaxE5 peptide efficiently permeabilizes membranes at acidic pH, showing low permeabilization neutral pH. Atomic force microscopy (AFM) imaging showed pH established several...
Abstract Membrane proteins play critical roles in disease and the disposition of many pharmaceuticals. A prime example is P-glycoprotein (Pgp) which moves a diverse range drugs across membranes out cell before therapeutic payload can be delivered. Conventional structural biology methods have provided valuable framework for comprehending complex conformational changes underlying Pgp function, also includes ATPase activity, but lack real-time information hinders understanding. Atomic force...