ABSTRACT Bud formation is an adaptive trait that temperate forest trees have acquired to facilitate seasonal synchronization. We characterized transcriptome‐level changes occur during bud of white spruce [ Picea glauca (Moench) Voss], a primarily determinate species in which preformed stem units contained within the apical constitute most next season's growth. Microarray analysis identified 4460 differentially expressed sequences shoot tips short day‐induced formation. Cluster revealed...

Plant response to stress is orchestrated by hormone signalling pathways including those activated jasmonates (JAs) and ethylene, both of which stunt root growth. COI1 a JA receptor required for the known responses this hormone. It was observed that coi1 mutant, largely unresponsive growth inhibition JAs, also partially ethylene its immediate precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), in light but not dark. Although ACC, other components signal perception pathway were not....

It has been suggested that cesium is absorbed from the soil through potassium uptake machineries in plants; however, not much known about perception mechanism and downstream response. Here, we report jasmonate pathway required plant response to cesium. Jasmonate biosynthesis mutant aos jasmonate-insensitive coi1-16 show clear resistance root growth inhibition caused by However, contents these mutants are comparable wild-type plants, indicating signaling involved uptake, but perception....

The accident at the Fukushima Daiichi nuclear power plant, which was one of most serious adverse effects Great East Japan Earthquake, accompanied by release a large quantity radioactive materials including (137)Cs to environment. In previous report, we developed and proposed cesium (Cs) fluorescent probe, "Cesium Green", that enables detection carbonate particles spraying an alcoholic solution Cesium Green probe. this paper, sensing activity probe investigated for its selectivity (by using...

Cesium (Cs+) is known to compete with the macronutrient potassium (K+) inside and outside of plants inhibit plant growth at high concentrations. However, detailed molecular mechanisms how Cs+ exerts its deleterious effects on K+ accumulation in are not fully elucidated. Here, we show that mutation a member major channel AKT1-KC1 complex renders Arabidopsis thaliana hypersensitive Cs+. Higher severity phenotype loss were observed for these mutants response than deficiency....

Abstract High concentrations of cesium (Cs + ) inhibit plant growth but the detailed mechanisms Cs uptake, transport and response in plants are not well known. In order to identify small molecules with a capacity enhance tolerance , chemical library screening was performed using Arabidopsis . Of 10,000 chemicals tested, five compounds were confirmed as enhancers. Further investigation quantum mechanical modelling revealed that one these reduced imidazole moiety this compound bound...

14-3-3 proteins are regulatory found in all eukaryotes and known to selectively interact with phosphorylated regulate physiological processes. Through an affinity purification screening, many light-related were recovered as candidate binding partners. Yeast two-hybrid analysis revealed that the kappa isoform (14-3-3κ) could bind PHYTOCHROME INTERACTING FACTOR3 (PIF3) CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). Further by vitro pull-down assay confirmed interaction between 14-3-3κ PIF3....

The red microalga Galdieria sp. is an extremophile that inhabits acidic hot sulphur springs and grows heterotrophically to a high cell density. These characteristics make suitable for commercial applications as stable mass production the key success in algae business. has great potential precious metal adsorbent provide sustainable, efficient environmentally benign method urban mining artisanal small-scale gold mining. efficiency selectivity capturing metals, palladium from solutions by...

Phytoremediation is optimised when plants grow vigorously while accumulating the contaminant of interest. Here we show that sulphur supply alleviates aerial chlorosis and growth retardation caused by cesium stress without reducing accumulation in Arabidopsis thaliana. This alleviation was not due to recovery cesium-induced potassium decrease plant tissues. Sulphur also alleviated sodium but deficiency stress. Cesium-induced root inhibition has previously been demonstrated as being mediated...

Abstract Phytoaccumulation is a technique to extract metals from soil utilising ability of plants. Cesium valuable metal while radioactive isotopes cesium can be hazardous. In order establish more efficient phytoaccumulation system, small molecules which promote plants accumulate were investigated. Through chemical library screening, 14 chemicals isolated as ‘cesium accumulators’ in Arabidopsis thaliana . Of those, methyl cysteinate, derivative cysteine, was found function within the plant...

Cesium has no known beneficial effects on plants and while have the ability to absorb it through root system, plant growth is retarded at high concentrations. Recently, we shown that potassium influx a channel complex AKT1-KC1 inhibited by cesium in Arabidopsis thaliana resultant reduction accumulation primary cause of growth. By contrast, major transporter, HAK5 whose function crucial under deficiency, was found be either not affected or complementary stress low affinity range. Here show...

Syringic acid, a phenolic compound, serves variety of beneficial functions in cells. acid increases plants response to cesium, and exogenous application syringic resulted significant attenuation cesium-induced growth defects Arabidopsis. In addition, cesium or also increased lignin deposition interfascicular fibers. To better understand the role attenuating defects, two mutants for Arabidopsis REDUCED EPIDERMAL FLUORESCENE 4 (REF4) fourteen laccase mutants, some which have lower levels...

Plants have developed mechanisms to adapt the potassium deficient conditions over years. In Arabidopsis thaliana , expression of a transporter HAK5 is induced in low as an adaptive response nutrient deficiency. order understand mechanism which regulated, full-length cDNA overexpressor gene hunting system was employed screening method. Of 40 genes recovered, At4g18280 found be dramatically potassium-deficiency and salt stress. overexpressing this showed higher enhanced growth. These plants...

Abstract Cesium (Cs) is found at low levels in nature but does not confer any known benefit to plants. Cs and K compete cells due the chemical similarity of potassium (K), can induce deficiency cells. In previous studies, we identified chemicals that increase tolerance Among them, a small compound (C 17 H 19 F 3 N 2 O ), named CsToAcE1, was confirmed enhance while increasing accumulation Treatment plants with CsToAcE1 resulted greater also alleviated Cs-induced growth retardation Arabidopsis...

The following section of the Materials and methods to be changed as follows Preparation constructs construction transgenic lines Arabidopsis lines (background Col gl1) were prepared identify whether distinct active sites on COI1 protein responsible for differential functional activation. To obtain LRR Fbox, different portions coding sequence PCR-amplified NcoI/SmaI fragments from construct containing entire in pPily (Ferrando et al., 2000; intron-tagged COI1::HiA, Devoto 2002), cloned...