A5034293517- Nanopore and Nanochannel Transport Studies
- Ion-surface interactions and analysis
- Fuel Cells and Related Materials
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Membrane-based Ion Separation Techniques
- RNA and protein synthesis mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Thermal properties of materials
- Advanced Thermodynamics and Statistical Mechanics
- Microfluidic and Bio-sensing Technologies
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Electrochemical Analysis and Applications
- Advanced Electron Microscopy Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Carbon Nanotubes in Composites
- Semiconductor materials and devices
- Force Microscopy Techniques and Applications
- 2D Materials and Applications
- Spectroscopy and Quantum Chemical Studies
- Electrostatics and Colloid Interactions
- Conducting polymers and applications
- Graphene research and applications
- Groundwater flow and contamination studies
Northeastern University
2013-2024
Salk Institute for Biological Studies
2020
Lawrence Livermore National Laboratory
2017-2018
Fast water transport through carbon nanotube pores has raised the possibility to use them in next generation of treatment technologies. We report that permeability 0.8-nanometer-diameter porins (CNTPs), which confine down a single-file chain, exceeds biological transporters and wider CNT by an order magnitude. Intermolecular hydrogen-bond rearrangement, required for entry into nanotube, dominates energy barrier can be manipulated enhance rates. CNTPs block anion transport, even at salinities...
We present a study of double- and single-stranded DNA transport through nanopores fabricated in ultrathin (2-7 nm thick) freestanding hafnium oxide (HfO2) membranes. The high chemical stability HfO2 enables long-lived experiments with <2 diameter pores that last several hours, which we observe >50 000 translocations no detectable pore expansion. Mean velocities are slower than comparable silicon nitride pores, providing evidence have favorable physicochemical interactions nucleic acids can...
Molybdenum disulfide (MoS2) flakes can grow beyond the edge of an underlying substrate into a planar freestanding crystal. When is in form aperture, reagent-limited nucleation followed by growth facilitate direct and selective MoS2 membranes. We have found conditions under which grows preferentially across micrometer-scale prefabricated solid-state apertures silicon nitride membranes, resulting sealed membranes that are one to few atomic layers thick. investigated structure purity our...
In recent years, nanopores have emerged as exceptionally promising single-molecule sensors due to their ability detect biomolecules at subfemtomole levels in a label-free manner. Development of high-throughput nanopore-based biosensor requires multiplexing nanopore measurements. Electrical detection, however, poses challenge, each circuit must be electrically independent, which complex nanofluidics and embedded electrodes. Here, we present an optical method for simultaneous measurements the...
Nanopores are single-molecule sensors that show exceptional promise as a biomolecular analysis tool by enabling label-free detection of small amounts sample. In this paper, we demonstrate nanopores capable detecting the conformation an antiviral RNA drug target. The hepatitis C virus uses internal ribosome entry site (IRES) motif in order to initiate translation docking ribosomes its host cell. IRES is therefore viable and important Drug-induced changes HCV motif, from bent straight...
It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure translocation kinetics of different species through ∼3 nm diameter synthetic nanopores. Each varies time scale with which it is deformed from equilibrium, as step translation. Using machine-learning algorithms, we can differentiate among five species, analyze ratios binary mixtures, and distinguish isoacceptors.
When light is used to excite electronic transitions in a material, nonradiative energy during relaxation often released the form of heat. In this work, we show that photoexcitation silicon nitride nanopore using focused visible laser results efficient localized photothermal heating, which reduces nearby electrolyte viscosity and increases ionic conductance. addition, strong thermal gradient pore vicinity produced, evidenced by finite-element simulations experimental observation both ion DNA...
Nanopores are a promising platform in next generation DNA sequencing. In this platform, an individual strand is threaded into nanopore using electric field, and enzyme-based ratcheting used to move the through detector. During process residual ion current pore measured, which exhibits unique levels for different base combinations inside pore. While approach has shown great promise, accuracy not optimal because four bases chemically comparable one another, leading small differences...
Human mitochondrial tRNAs (mt-tRNAs), critical for biogenesis, are frequently associated with pathogenic mutations. These mt-tRNAs have unusual sequence motifs and require post-transcriptional modifications to stabilize their fragile structures. However, whether a modification that stabilizes wild-type (WT) mt-tRNA would also its variants is unknown. Here we show the N1-methylation of guanosine at position 9 (m1G9) mt-Leu(UAA), while stabilizing WT tRNA, has destabilizing effect on MELAS...
Human mitochondrial tRNAs (mt-tRNAs), critical for biogenesis, are frequently associated with pathogenic mutations. These mt-tRNAs have unusual sequence motifs and require post-transcriptional modifications to stabilize their fragile structures. However, whether a modification that stabilizes wild-type (WT) mt-tRNA structure would also its variants is unknown. Here we show the