Prostate cancer is the most common solid malignancy in men, with 32,000 deaths annually. Piperine, a major alkaloid constituent of black pepper, has previously been reported to have anti-cancer activity variety cell lines. The effect piperine against prostate not currently known. Therefore, this study, we investigated anti-tumor mechanisms on androgen dependent and independent cells. Here, show that inhibited proliferation LNCaP, PC-3, 22RV1 DU-145 cells dose manner. Furthermore, Annexin-V...

Lymphatic filarial parasites survive within the lymphatic vessels for years despite complex immune environment surrounding them. Parasites possibly accomplish this by adopting various immunomodulatory strategies, which include release of glutathione-S-transferases (GSTs) that counteract oxidative free radicals produced host. Since GSTs appear to be critical survival in host, several studies evaluated potential parasite as vaccine candidates especially against schistosomiasis, fascioliasis...

This study describes a novel phage display method based on an iterative subtraction strategy to identify candidate vaccine antigens of Brugia malayi. A cDNA library the infective larval stage B. malayi expressed surface T7 was sequentially screened with sera samples from human subjects showing different manifestations disease. Antigens that selectively and specifically bind immune were then enriched using multi-step panning procedure. identified five antigens, four which previously reported...

A homologue of the mammalian translationally controlled tumor protein (TCTP) was cloned from human parasiteSchistosoma mansoni (SmTCTP). Sequence analysis showed that SmTCTP differed other reported TCTPs in having only one signature sequence. Subsequently, a T7 expression system and expressed as histidine-tagged fusion protein. Recombinant (rSmTCTP) has molecular mass ∼23 kDa with histidine tag. Further transcripts are all life cycle stages parasite within vertebrate hosts. Interestingly,...

Abstract Significant quantities of PGE2 were produced by cercariae Schistosoma mansoni following incubation with linoleic acid, a free fatty acid found on the surface skin. Cyclooxygenase (COX) 2 inhibitors failed to block this production, suggesting that different biochemical pathway may be involved in production parasite. In addition, parasites also able induce and IL-10 from human mouse keratinocytes. Analysis skin during migratory phases infection confirmed these vitro observations. COX2...

Praziquantel (PZQ) is the drug of choice for schistosomiasis and probably only highly effective currently available treating schistosomiasis-infected individuals. The mode action PZQ involves increasing calcium uptake parasite, resulting in tegumental damage death parasite. Despite its remarkable function, target has not been identified yet. To begin to understand where acts, this study we expressed cDNA library Schistosoma mansoni on surface T7 bacteriophages screened with labeled PZQ. This...

Yersinia pestis is the etiologic agent of bubonic and pneumonic plagues. It speculated that Y. hijacks antigen-presenting cells (APCs), such as dendritic (DCs) alveolar macrophages, in order to be delivered lymph nodes. However, how APCs initially capture bacterium remains uncharacterized. well known HIV-1 uses human DC-specific intercellular adhesion molecule-grabbing nonintegrin (DC-SIGN) (CD209) receptor, expressed by APCs, captured target cell, CD4+ lymphocytes. Several gram-negative...

High mobility group box 1 (HMGB1) was originally discovered as a chromatin-binding protein several decades ago. It is now increasingly evident that HMGB1 plays major role in disease conditions such atherosclerosis, diabetes, arthritis, sepsis, and cancer. intriguing how deregulation of can result myriad conditions. Interestingly, involved cell proliferation, angiogenesis, metastasis during cancer progression. Furthermore, has been demonstrated to exert intracellular extracellular functions,...

Antibodies are widely available and cost-effective research tools in life science, antibody conjugates now extensively used for targeted therapy, immunohistochemical staining, or vivo diagnostic imaging of cancer. Significant advances site-specific labeling technologies have enabled the production highly characterized homogenous biomedical purposes, some recent studies utilized to synthesize bifunctional with both drug delivery properties. While these important clinical safety efficacy such...

Toxoplasma gondii is the causative agent for toxoplasmosis. The rhoptry protein 1 (ROP1) secreted by rhoptry, an apical secretory organelle of parasite. ROP1 plays important role in host cell invasion. In this study, efficacy as a vaccine candidate against toxoplasmosis was evaluated through intramuscular or subcutaneous injection BALB/c mice followed immunological characterization (humoral- and cellular-mediated) lethal challenge virulent T. RH strain mice. Briefly, recombinant DNA plasmid...

Millions of people worldwide are infected with filarial nematodes, responsible for lymphatic filariasis (LF) and other diseases causing chronic disablement. Elimination programs have resulted in a substantial reduction the rate infection certain areas creating need improved diagnostic tools to establish robust population surveillance avoid LF resurgence. Glycans from parasitic helminths emerging as potential antigens use assays. However, despite its crucial role host–parasite interactions,...

Human colonic short-chain fatty acid (SCFA) absorption is associated with increased luminal pH and HCO3- enhanced Na+ absorption. Therefore, the mechanism of SCFA transport, its dependence on interactions Cl-/HCO3- Na+/H+ exchangers were characterized. Luminal membrane vesicles (LMV) isolated by divalent cation precipitation from organ donor colons used for n-butyrate transport. Uptake into human LMV was minimal even in presence an inward gradient, but outward gradient significantly uptake...

Skin-stage schistosomula of Schistosoma mansoni were found to secrete molecules that are pro-apoptotic for skin T lymphocytes as measured by annexin V staining, caspase-3 activity, caspase-8 activities, and DNA fragmentation. Caspase-8 activities in peaked ∼8 h activity ∼16 after exposure the parasite secretions. Subset analysis showed mainly CD4+ CD8+ cells (but not B cells) susceptible parasite-induced effect. In situ staining confirmed presence apoptotic around challenge parasites naive...

Filarial nematodes enjoy one of the longest life spans any human pathogen due to effective immune evasion strategies developed by parasite. Among various exhibited parasite, Interleukin 10 (IL-10) productions and IL-10 mediated suppression has significant negative impact on host system. Recently, we identified a small heat shock protein expressed Brugia malayi (BmHsp12.6) that can bind soluble receptor alpha (IL-10R) activate effects in cell lines. In this study show IL-10R binding region...

Over 2 million Americans are currently living with prostate cancer. Current treatment strategies inefficient in controlling the disease. This has made it necessary to identify newer for treating Use of active principles from medically important herbs proven be effective various forms cancers. Glycyrrhizin, a triterpene compound isolated roots licorice is shown exhibit potent vitro cytotoxic activity against several human cancer cell lines. No information available Therefore, this study, we...

Toxoplasmosis in humans and other animals is caused by the protozoan parasite Toxoplasma gondii . During process of host cell invasion parasitophorous vacuole formation tachyzoites, secretes Rhoptry protein 8 ( ROP8 ), an apical secretory organelle. Thus, important for pathogenesis T. The DNA was constructed into a pVAX-1 vaccine vector used immunizing BALB/c mice. Immunized mice developed immune response characterized significant antibody responses, antigen-specific proliferation spleen...