A5023495081- Parasitic Diseases Research and Treatment
- Insect symbiosis and bacterial influences
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Malaria Research and Control
- Neonatal Respiratory Health Research
- Mosquito-borne diseases and control
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Rare Diseases
- Vector-borne infectious diseases
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immune cells in cancer
- Viral Infections and Vectors
- Macrophage Migration Inhibitory Factor
- Respiratory Support and Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Complement system in diseases
- Chronic Lymphocytic Leukemia Research
- Air Quality and Health Impacts
- Nematode management and characterization studies
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
1983-2025
Society for Vascular Surgery
2023
Iowa City Public Library
2023
J. Craig Venter Institute
2007
Imperial College London
2007
University of Dundee
2007
New England Biolabs (United States)
1999-2007
Research Triangle Park Foundation
2005
Anna University, Chennai
2002
Online Mendelian Inheritance In Man (OMIM) is a public database of bibliographic information about human genes and genetic disorders. Begun by Dr. Victor McKusick as the authoritative reference in Man, it now distributed electronically National Center for Biotechnology Information (NCBI). Material OMIM derived from biomedical literature written his colleagues at Johns Hopkins University elsewhere. Each entry has full text summary phenotype and/or gene copious links to other resources such...
Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions people in the developing world. We have sequenced approximately 90 megabase (Mb) genome human filarial parasite Brugia malayi predict 11,500 protein coding genes 71 Mb robustly assembled sequence. Comparative analysis with free-living, model nematode Caenorhabditis elegans revealed that, despite these having maintained little conservation local synteny during 350 million years evolution, they...
Abstract TLR-mediated induction of cholesterol 25-hydoxylase transcription in dendritic cells and macrophages is dependent on IFN-α/IFN-β signaling through INF-αR STAT1. The oxysterol-producing enzyme CH25H plays an important role regulating lipid metabolism, gene expression, immune activation. In vitro experiments using a panel TLR agonists to activate BMDCs demonstrated that Ch25h expression induced rapidly, selectively, robustly by the ligands poly I:C LPS. mechanism TLR3- TLR4-induced...
ABSTRACT Filarial nematode parasites establish long-term chronic infections in the context of an antiparasite immunity that is strongly biased toward a Th2 response. The mechanisms lead to this bias filarial antigens are not clear, but one possibility produce molecules have capacity proactively modify their immunological environment. Here we report humans secrete homologue human proinflammatory cytokine macrophage migration inhibitory factor (MIF) has capability modifying activity...
Although vitamin A deficiency in children seems to increase susceptibility infection and community trials have shown that supplementation can reduce childhood mortality from infectious diseases, the underlying biological mechanisms are largely unknown. We conducted a randomised, double-masked, placebo-controlled clinical trial among West Java, Indonesia, determine whether is associated with abnormalities T-cell subsets affects subsets.We studied 55 aged 3-6 years—30 xerophthalmia 25 without....
Abstract Alternatively activated macrophages (M2) have an important function in innate immune responses to parasitic helminths, and emerging evidence also indicates these cells are regulators of systemic metabolism. Here we show a critical role for mTORC2 signalling the generation M2 macrophages. Abrogation by selective conditional deletion adaptor molecule Rictor inhibits while leaving classically (M1) intact. Selective prevents differentiation clearance helminth infection mice, abrogates...
Genome and exome sequencing can identify variants unrelated to the primary goal of sequencing. Detecting pathogenic associated with an increased risk a medical disorder enables clinical interventions improve future health outcomes in patients their at-risk relatives. The Clinical Resource, or ClinGen, aims assess actionability genes disorders as part larger effort build central resource information regarding relevance genomic variation for use precision medicine research.
Abstract Activation of B cells in the airways is now believed to be great importance immunity pathogens, and it participates pathogenesis airway diseases. However, little known about mechanisms local activation mucosa. We investigated expression members cell-activating TNF superfamily (B factor family (BAFF) a proliferation-inducing ligand (APRIL)) resting TLR ligand-treated BEAS-2B primary human bronchial epithelial (PBEC). In unstimulated cells, BAFF APRIL was minimal. mRNA significantly...
Using genome-wide expression profiles from persons either experimentally challenged with malaria-infected mosquitoes or naturally infected Plasmodium falciparum malaria, we present details of the transcriptional changes that occur infection and are commonly shared between subjects presymptomatic clinically apparent malaria distinguish these two groups. Toll-like receptor signaling through NF-kappaB pathways was significantly upregulated in both groups, as were downstream genes function...
ABSTRACT While it is well established that infection with the rodent hookworm Nippostrongylus brasiliensis induces a strongly polarized Th2 immune response, little known about innate host-parasite interactions lead to development of this robust immunity. We exploited transient pulmonary phase N. study responses induced by helminth parasite in wild-type (WT) and severe-combined deficient (SCID) BALB/c mice. Histological analysis demonstrated cellular infiltrates caused transit through lungs...
ABSTRACT To examine the hormonal and immunological mechanisms that mediate sex differences in susceptibility to malaria infection, intact gonadectomized (gdx) C57BL/6 mice were inoculated with Plasmodium chabaudi AS-infected erythrocytes, responses infection monitored. In addition reduced mortality, females recovered from infection-induced weigh loss anemia faster than males. Expression microarrays real-time reverse transcription-PCR revealed gonadally exhibited higher expression of...
Abstract Estrogens increase aspects of innate immunity and contribute to sex differences in the prevalence autoimmune diseases response infection. The goal present study was assess whether exposure 17β-estradiol (E2) affects development function bone marrow-derived dendritic cells determine similar changes are observed CD11c+ splenocytes exposed E2 vivo. facilitated differentiation BM precursor into functional CD11c+CD11b+MHC class II+ (DCs) with increased expression costimulatory molecules...
Abstract To characterize the extent and impact of ancestry-related biases in precision genomic medicine, we use 642 whole-genome sequences from Consortium on Asthma among African-ancestry Populations Americas (CAAPA) project to evaluate typical filters databases. We find significant correlations between estimated African ancestry proportions number variants per individual all variant classification sets but one. The source these is highlighted more detail by looking at interaction filtering...
We have used a tag sequencing approach to survey genes expressed in the third stage infective larvae of human filarial nematode parasite Brugia malayi. RNA was isolated from late vector-stage L3 after days 9 or 10 infection mosquitos, and converted cDNA by reverse transcriptase. Double-stranded produced either conventional methods (non-SL library) PCR using spliced leader (SL1) oligo(dT) primers (SL library). Two clone libraries (one SL one non-SL cDNAs) were constructed lambda ZapII. A set...
Cytokines are the molecular messengers of vertebrate immune system, coordinating local and systemic responses to infective organisms. We report here functional structural data on cytokine-like proteins from a eukaryotic pathogen. Two homologues human cytokine macrophage migration inhibitory factor (MIF) have been isolated parasitic nematode Brugia malayi. Both molecules (Bm-MIF-1 Bm-MIF-2) show parallel functions MIF. They chemotactic for monocytes activate them produce IL-8, TNF-alpha,...
Filarial nematode parasites are a serious cause of morbidity in humans and animals. Identification filarial infection using traditional morphologic criteria can be difficult lead to misdiagnosis. We report on polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP)–based method detect differentiate broad range species single PCR. The first internal transcribed spacer 1 (ITS1) along with the flanking 18S 5.8S ribosomal DNA (rDNA) were isolated cloned from Wuchereria...