A5102910638- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Cardiovascular Function and Risk Factors
- Glaucoma and retinal disorders
- Corneal surgery and disorders
- Heart Failure Treatment and Management
- Genetic Associations and Epidemiology
- Tissue Engineering and Regenerative Medicine
- Intraocular Surgery and Lenses
- Muscle Physiology and Disorders
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Molecular Research
- Cardiac Imaging and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Cardiac Valve Diseases and Treatments
- Epigenetics and DNA Methylation
- Protein Kinase Regulation and GTPase Signaling
- Carbohydrate Chemistry and Synthesis
- Congenital heart defects research
- Retinal Development and Disorders
- Genetic factors in colorectal cancer
- Genetic diversity and population structure
- IL-33, ST2, and ILC Pathways
- Genetic and Kidney Cyst Diseases
- Ear and Head Tumors
Illumina (United States)
2008-2024
Hackensack Meridian Health
2023
Center for Discovery
2023
Minneapolis Institute of Arts
2021
Minnesota Eye Consultants
2019-2020
University of Minnesota
2020
Queensland Health
2014
Duke University
2011-2013
Toronto Public Health
2012
University of Utah
2004-2010
Muscle and its connective tissue are intimately linked in the embryo adult, suggesting that interactions between these tissues crucial for their development. However, study of muscle has been hindered by lack molecular markers genetic reagents to label fibroblasts. Here, we show transcription factor Tcf4 (transcription 7-like 2; Tcf7l2) is strongly expressed fibroblasts Tcf4GFPCre mice allow manipulation Using this new reagent, find critically regulate two aspects myogenesis: fiber type...
HIV-1 integrase is an attractive target for anti-retroviral chemotherapy, but to date no clinically useful inhibitors have been developed. We screened diverse marine natural products compounds active against in vitro and found a series of ascidian alkaloids, the lamellarins, that show selective inhibition. A new member family named lamellarin α 20-sulfate (1), structure which was determined from spectroscopic data, displayed most favorable therapeutic index. The site action on protein mapped...
Rituximab is an important therapeutic for Waldenstrom's macroglobulinemia (WM). Polymorphisms in FcgammaRIIIA (CD16) receptor expression modulate human immunoglobulin G1 binding and antibody-dependent cell-mediated cytotoxicity, may therefore influence responses to rituximab.Sequence analysis of the entire coding region was undertaken 58 patients with WM whose outcomes after rituximab were known.Variations five codons identified. Two commonly observed (FcgammaRIIIA-48 FcgammaRIIIA-158)...
Repair of mature skeletal muscle is mediated by adult progenitors. Satellite cells have long been recognized as playing a major role in repair, whereas side population (SP) more recently identified contributing to this process. The developmental source these two progenitor populations has considerably debated. We explicitly tested and quantified the contribution embryonic somitic populations. Chick were labeled using replication-defective retroviruses or quail/chick chimeras, mouse crossing...
Bioluminescent reporter genes are sensitive in situ tools for following disease progression preclinical models, albeit they subject to scattering and absorption deep tissues. We have generated a bicistronic Cre/LoxP mouse line that pairs the expression of firefly luciferase with quantifiable human placental alkaline phosphatase is secreted into serum (SeAP). With use this dual-modality bioreporter novel, inducible Pax7-CreER tracking muscle satellite cells, we demonstrate longitudinal...
Tissue Doppler-derived indices of strain (epsilon) and rate (SR) have been developed to assess regional cardiac function. However, the effect left ventricular (LV) size on epsilon SR has not studied in depth. The aim this study was what extent heart influence or SR.In 21 anaesthetized pigs ranging from 12.5 70.0 kg, tissue SR, haemodynamic parameters, were assessed during controlled rates different loading conditions. dP/dt did correlate pig weight, suggesting constant contractility growth....
Abstract To characterize the extent and impact of ancestry-related biases in precision genomic medicine, we use 642 whole-genome sequences from Consortium on Asthma among African-ancestry Populations Americas (CAAPA) project to evaluate typical filters databases. We find significant correlations between estimated African ancestry proportions number variants per individual all variant classification sets but one. The source these is highlighted more detail by looking at interaction filtering...
ABSTRACT Integration of the human immunodeficiency virus type 1 (HIV-1) cDNA is a required step for viral replication. Integrase, virus-encoded enzyme important integration, has not yet been exploited as target clinically useful inhibitors. Here we report on identification new polyhydroxylated aromatic inhibitors integrase including ellagic acid, purpurogallin, 4,8,12-trioxatricornan, and hypericin, last which known to inhibit These compounds others were characterized in assays with subviral...
The Infinium DNA Methylation BeadChips have significantly contributed to population-scale epigenetics research by enabling epigenome-wide trait association discoveries. Here, we design, describe, and experimentally verify a new iteration of this technology, the Screening Array (MSA), focus on human screening discovery. This array utilizes extensive data from previous platform-based studies (EWAS). It incorporates knowledge latest single-cell cell type-resolution whole genome methylome...
Integration is a required step in HIV replication, but as yet no inhibitors of the integration have been developed for clinical use. Many identified that are active against purified viral-encoded integrase protein; these, many contain catechol moiety. Though this substructure contributes potency inhibitors, it associated with toxicity and so utility catechol-containing has questioned. We synthesized tested systematic series derivatives inhibitor (1) goal identifying isosteres support...
Abstract Background Tumor initiation has been attributed to haploinsufficiency at a single locus for large number of cancers. Patched1 ( Ptc1 ) was one the first such loci, and asserted lead medulloblastoma rhabdomyosarcoma in mice. Procedure To study role cerebellar tumor development create preclinical therapeutic platform, we have generated conditional model by inactivating Pax7 ‐expressing cells cerebellum. Results These mice developed exclusively medulloblastoma. We show that despite...
To assess the effects of Rho-associated kinase (ROCK) inhibition on intraocular penetration timolol maleate.Ex vivo porcine corneal maleate, sotalol hydrochloride, or brinzolamide incubated with without Y-27632 was determined in vertical Franz diffusion cells. The effect ROCK vasodilation conjunctival vasculature assessed by scanning electron microscopy (SEM) and immunohistochemical staining subsequent laser-scanning confocal (LSCM). Experiments were conducted New Zealand White (NZW) rabbits...
Tuberculosis meningitis (TBM) is essentially treated with the first-line regimen used against pulmonary tuberculosis, a prolonged continuation phase. However, clinical outcomes are poor in comparison, for reasons that only partially understood, highlighting need improved preclinical tools to measure drug distribution and activity at site of disease. A predictive animal model TBM would also be great value prioritize promising regimens tested trials, given healthy state development pipeline...
Mouse models are critical tools in tuberculosis (TB) research. Recent studies have demonstrated that the wild mouse gut microbiota promotes host fitness and improves disease resistance. Here we examine whether alters immunopathology of TB BALB/c mice. Conventional mice (LabC) born to germ-free mothers reconstituted with (WildR) were used our studies. WildR controlled initial infection better than LabC The microbial communities had similar richness but significantly different composition...
Abstract Millions of Formalin Fixed Paraffin Embedded (FFPE) cancer tissue samples currently present in the US and worldwide provide an enormous, invaluable repository to discovery biomarkers research, drug development, diagnosis treatment diseases. However, processes fixation storage FFPE leads degradation base modification with chemical residues. This degraded DNA is inefficiently amplified by Infinium® whole genome amplification (WGA) step leading poor performance wide methylation...
A primary goal of The C onsortium on sthma among frican-ancestry P opulations in the mericas (CAAPA) is to develop an ‘African Diaspora Power Chip’ (ADPC), a genotyping array consisting tagging SNPs, useful comprehensively identifying African specific genetic variation. This designed based novel variation identified 642 CAAPA samples ancestry with high coverage whole genome sequence data (~30x depth). extends pattern catalogued 1000 Genomes and Exome Sequencing Projects spectrum populations...
Early invasive treatments in patients with acute heart failure (AHF) are critical components to improve outcome. We aimed establish if such were applied according existing guidelines and also assess the subsequent mortality complete AHF population. All admitted intensive care unit/coronary unit during years 2003-2004 (n=302) retrospectively reviewed classified European Society of Cardiology. Invasive revascularization was more frequently cardiogenic shock following coronary syndromes (78%,...
Purpose: To evaluate the ex vivo safety and efficacy of using paired peripheral incisions to achieve a triple scroll conformation that facilitates unscrolling in Descemet membrane endothelial keratoplasty (DMEK). Methods: The adding DMEK grafts was evaluated by assessing cell loss (ECL) risk tearing. ECL measured calcein-AM staining after incisions. tearing comparing incision lengths before simulated surgery cadaveric eyes. Efficacy scrolling pattern width with different (0.0 mm, 0.5 1.0...