A5045587265- Toxoplasma gondii Research Studies
- Malaria Research and Control
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Drug Transport and Resistance Mechanisms
- Invertebrate Immune Response Mechanisms
- HIV Research and Treatment
- Gut microbiota and health
- Vibrio bacteria research studies
- vaccines and immunoinformatics approaches
- Rabies epidemiology and control
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Complement system in diseases
University of Iowa
2020-2023
Washington University in St. Louis
2014-2019
University of Würzburg
2017
Abstract Research into post‐transcriptional control of mRNAs by small noncoding RNAs (sRNAs) in the model bacteria Escherichia coli and Salmonella enterica has mainly focused on sRNAs that associate with RNA chaperone Hfq. However, recent discovery protein ProQ as a common binding partner stabilizes distinct large class structured suggests additional regulons exist these organisms. The cellular functions molecular mechanisms new ProQ‐dependent are largely unknown. Here, we report Typhimurium...
Toxoplasma gondii contains an expanded number of calmodulin (CaM)-like proteins whose functions are poorly understood. Using a combination CRISPR/Cas9-mediated gene editing and plant-like auxin-induced degron (AID) system, we examined the roles three apically localized CaMs. CaM1 CaM2 were individually dispensable, but loss both resulted in synthetic lethal phenotype. CaM3 was refractory to deletion, suggesting it is essential. Consistent with this prediction degradation blocked growth....
Apicomplexan parasites are typified by an apical complex that contains a unique microtubule-organizing center (MTOC) organizes the cytoskeleton. In apicomplexan such as Toxoplasma gondii, includes spiral cap of tubulin-rich fibers called conoid. Although described ultrastructurally, composition and functions conoid largely unknown. Here, we localize 11 previously undescribed proteins in T. gondii identify essential component named protein hub 1 (CPH1), which is conserved parasites. CPH1...
ABSTRACT Apicomplexan parasites actively invade host cells using a mechanism predicted to be powered by parasite actin-dependent myosin motor. In the model apicomplexan Toxoplasma gondii , inducible knockout of actin gene, ACT1 was recently demonstrated limit but not completely abolish invasion. This observation has led provocative suggestion that T. possesses alternative, ACT1-independent invasion pathways. Here, we dissected residual invasive ability Δ act1 parasites. Surprisingly, were...
Abstract Production of IFN-γ by CD4 T cells is widely theorized to control Plasmodium parasite burden during blood-stage malaria. Surprisingly, the specific and crucial mechanisms through which this highly pleiotropic cytokine acts confer protection against malarial disease remain largely untested in vivo. Here we used a cell–restricted Cre-Lox excision mouse model test whether how cell–derived controls Although complete absence compromised acute chronic, recrudescent infections with P. c....
Radiation-attenuated sporozoite (RAS) vaccination offers hope for global malaria control through induction of protective liver-stage-specific memory CD8 T cells. Effective RAS regimens exist; however, widespread implementation remains unfeasible. A key difficulty resides in the need to administer three or more doses i.v. achieve sufficient immunity. Strategies reduce number are therefore desirable. Here we used mice model human immune responses a single, suboptimal weight-normalized dose...
Toxoplasma gondii is a parasitic protist and opportunistic pathogen of humans. In healthy humans, acute toxoplasmosis typically benign, causing only flu‐like symptoms. However, parasites are not cleared when this infection resolves, the host instead left chronically infected for life. The chronic caused by slow‐growing parasite cysts that predominantly located in long‐lived, differentiated cells, including those central nervous system skeletal muscle. If immunity wanes host, growth can...