A5071272403- Chromatin Remodeling and Cancer
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Graphene and Nanomaterials Applications
- Bone Tissue Engineering Materials
- Infant Nutrition and Health
- Electrochemical Analysis and Applications
- Electrospun Nanofibers in Biomedical Applications
- EEG and Brain-Computer Interfaces
- Analytical Chemistry and Sensors
- Advanced Memory and Neural Computing
- Neuroscience and Neural Engineering
- Neonatal Respiratory Health Research
- RNA modifications and cancer
- Advanced Fluorescence Microscopy Techniques
- Protein Hydrolysis and Bioactive Peptides
- Polymer Science and Applications
- Dyeing and Modifying Textile Fibers
- Digestive system and related health
- Dendrimers and Hyperbranched Polymers
- Advanced Theoretical and Applied Studies in Material Sciences and Geometry
- Epigenetics and DNA Methylation
- Textile materials and evaluations
- Protein Degradation and Inhibitors
- Nanoparticle-Based Drug Delivery
Kurchatov Institute
2016-2024
Institute of Gene Biology
2018-2023
Russian Academy of Sciences
2021
Genetically encoded calcium indicators (GECIs) are mainly represented by two- or one-fluorophore-based sensors. One type of two-fluorophore-based sensor, carrying Opsanus troponin C (TnC) as the Ca2+-binding moiety, has two binding sites for ions, providing a linear response to ions. One-fluorophore-based sensors have four but better suited in vivo experiments. Herein, we describe novel design GECI with sites. The engineered called NTnC, uses TnC inserted mNeonGreen fluorescent protein....
The Polybromo-associated BAF (BRG1- or BRM-associated factors) (PBAF) chromatin-remodeling complex is essential for transcription in mammalian cells. In this study, we describe a novel variant of the PBAF from differentiated neuronal cells, called dcPBAF, that differs canonical existing proliferating neuroblasts. We adult neurons, specific subunit PBAF, PHF10, replaced by PHF10 isoform lacks N- and C-terminal domains (called PHF10D). addition, dcPBAF does not contain BRD7 subunit. binds...
Lactoferrin (Lf) is a multifunctional protein from the transferrin family. Of particular interest ability of Lf to affect wide range neuronal processes by modulating expression genes involved in long-term neuroplasticity. The immediate early gene c-fos that rapidly activated response external influences, and its product, transcription factor c-Fos, widely used as marker plasticity. present study aims examine effect human on induction c-Fos primary mouse cultures after stimulation determine...
Abstract The PBAF chromatin remodeling complex of the SWI/SNF family plays a critical role in regulation gene expression during tissue differentiation and organism development. subunits have domains responsible for binding to N-terminal histone sequences. It determines specificity chromatin. PHF10, specific subunit complex, contains DPF domain, which is unique interaction domain. A PHF10 isoform that lacks domain also present vertebrate cells. This work shows neuronal muscle human mouse...
Chromatin remodelling multiprotein complexes play an important role in regulation of gene expression embryogenesis and the adult organism. Mutations subunits are often lethal or lead to developmental defects. Complexes consist core a specific module. The consists ATPase structure subunits, module necessary for chromatin binding. PHF10 (PHD finger protein 10) is subunit PBAF (polybromo-associated BAF) complex subfamily. Conserved highly regulated ubiquitously expressed mammals as four...
The PBAF chromatin remodeling complex of the SWI/SNF family plays a critical role in regulation gene expression during tissue differentiation and organism development. subunits have domains responsible for binding to N-terminal histone sequences. It determines specificity chromatin. PHF10, specific subunit complex, contains DPF domain, which is unique interaction domain. A PHF10 isoform that lacks domain also present vertebrate cells. This work shows neuronal muscle human mouse cells,...
Целью настоящей работы стало исследование влияния лактоферрина (Лф) человека на экспрессию транскрипционного фактора c-Fos в первичных нейрональных культурах после физиологической стимуляции, определение клеточной локализации Лф и возможной колокализации экзогенного белка с индуцированной экспрессией c-Fos. Методы. Первичные диссоциированные клеточные культуры получали из гиппокампа головного мозга новорожденных мышей (Р0-Р1) линии С57Вl/6. Индукцию экспрессии клетках осуществляли путем...
The PBAF chromatin remodeling complex interacts with many transcriptional activators and is recruited to target regions. plays an important role in maintaining modifying the structure mammalian cells. A subunit of complex, PHF10 transcription factor, required for proliferation neuronal precursors early stages mouse brain development gene expression differentiated neurons. We showed that protein product response </>c-FOS, c-FOS activator, which expressed induction long-term potentiation...
Устойчивость спонтанной электрической активности нейронных сетей in vitro Stability of spontaneous electrical activity neural networks vitroUsing brain-computer interfaces, one can both read data from and transmit them to the brain.However, these are only a set sensor system signals not knowledge or experience.Neural basis for cognitive simulate processes similar learning vitro.In this work we tested hypothesis network's ability learn by detecting deviations its stereotypical modifying way...