A5058628929- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- RNA modifications and cancer
- Chromatin Remodeling and Cancer
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Heat shock proteins research
- interferon and immune responses
- S100 Proteins and Annexins
- Cytokine Signaling Pathways and Interactions
- Cancer Mechanisms and Therapy
- Cancer-related gene regulation
- Plant Molecular Biology Research
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Chromosomal and Genetic Variations
- Invertebrate Immune Response Mechanisms
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
Engelhardt Institute of Molecular Biology
2016-2025
Russian Academy of Sciences
2007-2023
Institute of Gene Biology
2012-2022
Institute of Bioorganic Chemistry
2018
Institute of Molecular Genetics
2015
Skolkovo Foundation
2012
University of Oslo
1999-2010
Research Centre for Medical Genetics
2007
Institute of Cell Biophysics
2005
Ludwig-Maximilians-Universität München
2001
Metazoan E(y)2/ENY2 is a multifunctional protein important for transcription activation and mRNA export, being component of SAGA/TFTC the export complex AMEX. Here, we show that ENY2 in Drosophila also stably associated with THO, involved mRNP biogenesis. The ENY2–THO required normal development, functioning independently on SAGA THO arrive transcribed region hsp70 gene after its activation, plays an role recruitment. no direct association elongating RNA polymerase II. Recruitment occurs by...
Despite increasing data on the properties of replication origins, molecular mechanisms underlying origin recognition complex (ORC) positioning in genome are still poorly understood. The Su(Hw) protein accounts for activity best-studied Drosophila insulators. Here, we show that recruits histone acetyltransferase SAGA and chromatin remodeler Brahma to Su(Hw)-dependent insulators, which gives rise regions with low nucleosome density creates conditions ORC binding. Depletion leads a dramatic...
We have isolated a novel Drosophila (d) gene coding for two distinct proteins via alternative splicing: homologue of the yeast adaptor protein ADA2, dADA2a, and subunit RNA polymerase II (Pol II), dRPB4. Moreover, we identified another in genome encoding second ADA2 (dADA2b). The dADA2 homologues, as well many putative homologues from different species, all contain, addition to ZZ SANT domains, several evolutionarily conserved domains. dada2a/rpb4 dada2b genes are differentially expressed at...
Transcription activation by RNA polymerase II is a complicated process driven combined, precisely coordinated action of wide array coactivator complexes, which carry out chromatin-directed activities and nucleate the assembly preinitiation complex on promoter. Using various techniques, we have shown existence stable supercomplex consisting chromatin-remodeling factor Brahma (SWI/SNF) transcription initiation TFIID, named BTFly (Brahma TFIID in one assembly). The coupling mediated SAYP...
The presence of general transcription factors and other coactivators at the Drosophila hsp70 gene promoter in vivo has been examined by polytene chromosome immunofluorescence chromatin immunoprecipitation endogenous heat-shock loci or a promoter-containing transgene. These studies indicate that is already occupied TATA-binding protein (TBP) several TBP-associated (TAFs), TFIIB, TFIIF (RAP30), TFIIH (XPB), TBP-free/TAF-containg complex (GCN5 TRRAP), Mediator subunit 13 before heat shock....
Drosophila SAYP, a homologue of human PHF10/BAF45a, is metazoan coactivator associated with Brahma and essential for its recruitment on the promoter. The role SAYP in DHR3 activator-driven transcription ftz-f1 gene, member ecdysone cascade was studied. In repressed state presence DHR3, Pol II complex pre-recruited promoter; starts but paused 1.5 kb downstream promoter, forming 'nucleosomal barrier' (a region high nucleosome density) ahead II. depletion leads to removal Brahma, thereby...
The PBAF subtype of the mammalian chromatin remodeling SWI/SNF complex has wide and diverse functions in transcription regulation development, being both activator repressor. However, a mechanism accounting for such functional diversity remains unclear. Human PHF10/BAF45a subunit plays an important role brain development but not been studied sufficiently. We have shown that PHF10 gene encodes 2 types evolutionarily conserved, ubiquitously expressed isoforms are incorporated into mutually...
Genetic constructs containing the human lactoferrin (hLf) gene were created within a joint program of Russian and Belorussian scientists. Using these constructs, transgenic mice bred (the maximum hLf concentration in their milk was 160 g/L), goats also generated (up to 10 g/L milk). Experimental goatherds that produced bred, recombinant found be identical natural protein its physical chemical properties. These properties included electrophoretic mobility, isoelectric point, recognition by...
TFIID is a multiprotein complex composed of the TATA binding protein (TBP) and TBP-associated factors (TAF(II)s). The to promoter first step RNA polymerase II preinitiation assembly on protein-coding genes. Yeast (y) human (h) complexes contain 10 13 TAF(II)s. Biochemical studies suggested that Drosophila (d) only eight TAF(II)s, leaving number yeast TAF(II)s (e.g., hTAF(II)55, hTAF(II)30, hTAF(II)18) without known homologues. We demonstrate has not one but two hTAF(II)30 homologues,...
AbstractThe Drosophila TATA box-binding protein (TBP)-related factor 2 (TRF2 or TLF) was shown to control a subset of genes different from that controlled by TBP. Here, we have investigated the structure and functions trf2 gene. We demonstrate it encodes two isoforms: previously described 75-kDa TRF2 newly identified 175-kDa version in which same sequence is preceded long N-terminal domain with coiled-coil motifs. Chromatography embryo extracts revealed part multiprotein complex also...
SAGA/TFTC is a histone acetyltransferase complex that has second enzymatic activity because of the presence deubiquitination module (DUBm). Drosophila DUBm consists Sgf11, ENY2 and Nonstop proteins. We show Sgf11 other DUBm-independent functions. It associates with Cbp80 component cap-binding thereby recruited onto growing messenger ribonucleic acid (mRNA); it also interacts AMEX mRNA export essential for hsp70 export, as well general from nucleus. Thus, functions both SAGA biogenesis machinery.
The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates pre-replication assembly. In literature, there are several reports interaction ORC different RNAs. Here, we demonstrate for first time a direct THSC/TREX-2 mRNA nuclear export complex. was purified from Drosophila embryonic extract found to bind fraction ORC. This occurred via subunits essential viability. Also, associated mRNP, which facilitated by TREX-2. interacted Nxf1 receptor...
The germline-specific role of telomeres consists chromosome end elongation and proper segregation during early developmental stages. Despite the crucial in germ cells, little is known about telomere biology germline. We analyzed homeostasis Drosophila female germline embryos. A novel function deadenylase complex Ccr4-Not telomeric transcript surveillance mechanism reported. Depletion components causes strong derepression retroelement HeT-A accompanied by poly(A) tail. Dysfunction...
Weak hypomorph mutations in the enhancer of yellow genes, e(y)1 and e(y)2, Drosophila melanogaster were discovered during search for genes involved organization interaction between enhancers promoters.Previously, gene was cloned found to encode TAF II 40 protein.Here we e(y)2 demonstrated that it encoded a new ubiquitous evolutionarily conserved transcription factor.The is located at 10C3 (36.67) region expressed all stages development.It encodes 101-amino-acid protein, e(y)2.Vertebrates,...
The enhancer of yellow 1 gene, e(y)1, ofDrosophila melanogaster has been cloned and demonstrated to encode the TAFII40 protein. e(y)1 gene is expressed in females much more strongly than males due accumulation mRNA ovaries. Two differente(y)1 mutations have obtained. Thee(y)1ul mutation, induced by insertion ofStalker into coding region, leads replacement 25 carboxy-terminal amino acids 17 encoded theStalker sequences a decrease thee(y)1 transcription level. latter main cause dramatic...