A5101689178- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Photochromic and Fluorescence Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Retinal Development and Disorders
- Melanoma and MAPK Pathways
- Nanoplatforms for cancer theranostics
- Radiopharmaceutical Chemistry and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Bioactive Compounds and Antitumor Agents
- HIV Research and Treatment
- Plasmonic and Surface Plasmon Research
- bioluminescence and chemiluminescence research
- Plant Pathogens and Fungal Diseases
- Autophagy in Disease and Therapy
- Microbial Metabolic Engineering and Bioproduction
- Click Chemistry and Applications
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- ATP Synthase and ATPases Research
- Photoreceptor and optogenetics research
- Innovation and Socioeconomic Development
- Optical Coatings and Gratings
Peking University
2025
Peking University Cancer Hospital
2025
University of Pittsburgh
2016-2023
Tianjin Institute of Industrial Biotechnology
2022-2023
Chinese Academy of Sciences
2022-2023
Tianjin University of Science and Technology
2023
Massachusetts Institute of Technology
2021-2022
McGovern Institute for Brain Research
2021-2022
We developed a new method for the conditional regulation of CRISPR/Cas9 activity in mammalian cells and zebrafish embryos using photochemically activated, caged guide RNAs (gRNAs). Caged gRNAs are generated by substituting four nucleobases evenly distributed throughout 5'-protospacer region with during synthesis. Caging confers complete suppression gRNA:dsDNA-target hybridization rapid restoration function upon optical activation. This tool offers simplicity programmability design, high...
The type III-E CRISPR-Cas7-11 effector binds a CRISPR RNA (crRNA) and the putative protease Csx29 catalyzes crRNA-guided cleavage. We report cryo-electron microscopy structures of Cas7-11-crRNA-Csx29 complex with without target (tgRNA), demonstrate that tgRNA binding induces conformational changes in Csx29. Biochemical experiments revealed tgRNA-dependent cleavage accessory protein Csx30 by Reconstitution system bacteria showed yields toxic fragments cause growth arrest, which is regulated...
B7-H3 or CD276 is notably overexpressed in various malignant tumor cells humans, with extremely high expression rates. The development of a radiotracer that targets may provide universal tumor-specific imaging agent and allow the noninvasive assessment whole-body distribution B7-H3-expressing lesions. We enhanced optimized structure an affibody (ABY) to create radiolabeled [68Ga]Ga-B7H3-BCH, then, we conducted both foundational experiments clinical translational studies. [68Ga]Ga-B7H3-BCH...
Abstract Programmable and multiplexed genome integration of large, diverse DNA cargo independent repair remains an unsolved challenge editing. Current gene approaches require double-strand breaks that evoke damage responses rely on pathways are inactive in terminally differentiated cells. Furthermore, CRISPR-based bypass double stranded breaks, such as Prime editing, limited to modification or insertion short sequences. We present Addition via Site-specific Targeting Elements, PASTE, which...
Abstract We developed a new method for the conditional regulation of CRISPR/Cas9 activity in mammalian cells and zebrafish embryos using photochemically activated, caged guide RNAs (gRNAs). Caged gRNAs are generated by substituting four nucleobases evenly distributed throughout 5′‐protospacer region with during synthesis. Caging confers complete suppression gRNA:dsDNA‐target hybridization rapid restoration function upon optical activation. This tool offers simplicity programmability design,...
Abstract We have developed a new tool for the optical control of cellular ATP concentrations with photocaged adenylate kinase (Adk). The Adk is generated by substituting catalytically essential lysine hydroxycoumarin‐protected through site‐specific unnatural amino acid mutagenesis in both E. coli and mammalian cells. Caging critical residue offers complete suppression Adk's phosphotransferase activity rapid restoration its function vitro vivo upon stimulation. Light‐activated renders faster...
The selective pressure imposed by extrinsic death signals and stressors adds to the challenge of isolating interpreting roles proteins in stress-activated signaling networks. By expressing a kinase with activating mutations caged lysine blocking active site, we can rapidly switch on catalytic activity light monitor ensuing dynamics. Applying this approach MAP 6 (MKK6), which activates p38 subfamily MAPKs, found that decaging MKK6 fibroblasts is sufficient trigger apoptosis p38-dependent...
Genetic engineering is one of the most effective methods to obtain fungus strains with desirable traits. However, in some filamentous fungi, targeted gene deletion transformant screening on primary transformation plates time-consuming and laborious due a relatively low rate homologous recombination. A strategy that compensates for recombination by improving efficiency was performed F. venenatum TB01. In this study, visualized system could easily distinguish fluorescent randomly inserted...
Abstract The diversity of cell types and states can be scalably measured defined by expressed RNA transcripts. However, approaches to programmably sense respond the presence specific RNAs within living biological systems with high sensitivity are lacking. sensors that gate expression reporter or cargo genes would have diverse applications for basic biology, diagnostics therapeutics enabling cell-state control transgene expression. Here, we engineer a novel programmable RNA-sensing...
Thermophilic endoglucanases have become of significant interest for effectively catalyzing the hydrolysis cellulose. Myceliophthora thermophila is an ideal source thermophilic enzymes. Interestingly, different hosts differently express same In this study, we successfully overexpressed endoglucanase (MtEG5-1) from M. in methylotrophic yeast, Pichia pastoris GS115, via electroporation. We found that purified MtEG5-1 exhibited optimum activity levels at pH 5 and 70 °C, with 88% thermal...
Abstract We developed a method to control gene activation and base editing in mammalian cells zebrafish embryos via photochemically activated, caged guide RNAs. Four evenly distributed, nucleobases are installed the 5’‐protospacer region of gRNA block interaction between dCas9:gRNA complex dsDNA target. Upon light activation, gRNA:dsDNA hybridization is restored catalytically dead Cas9‐based CRISPR or achieved. Caged gRNAs customizable design, offer spatiotemporal transcription both embryos,...
Abstract The type III-E Cas7-11 effector nuclease forms a complex with CRISPR RNA (crRNA) and the putative caspase-like protease Csx29, catalyzes crRNA-guided target cleavage, has been used for targeting in eukaryotic cells. Here, we report cryo-electron microscopy structures of Cas7-11–crRNA–Csx29 without RNA, demonstrate that binding induces conformational change Csx29 results activation. Biochemical analysis confirmed Cas7-11-bound cleaves Csx30 RNA-dependent manner. Reconstitution system...
Abstract Das CRISPR/Cas9‐Endonukleasesystem, das aus einer Guide‐RNA zur Erkennung Zielsequenz der DNA und dem Cas9‐Nukleaseprotein Bindung Prozessierung Ziel‐DNA besteht, wurde intensiv erforscht findet bereits breite Anwendung im Gen‐Editing, Synthesebiologie transkriptionellen Modulation in Zellen Tieren. Mit Ziel präziseren Genmodifizierung Weiterentwicklung des CRISPR/Cas9‐Systems zu einem räumlich‐zeitlich kontrollierbaren Genregulationssystem wurden kürzlich mehrere Ansätze...
Stress-activated signaling pathways orchestrate cellular behaviors and fates. Studying the precise role(s) of stress-activated protein kinases is challenging, because stress conditions induce adaptation impose selection pressure. To meet this challenge, we have applied an optogenetic system with a single plasmid to express light-activated p38α or its upstream activator, MKK6, in conjunction live-cell fluorescence microscopy. In starved cells, decaging constitutively active MKK6 by brief...
Protein isoforms are difficult to differentiate in a cellular context. Here, we describe the acute light control of individual mitogen-activated protein kinase (MAPK) p38, revealing novel point crosstalk between two MAPK pathways.
Signal transduction is an essential mechanism of cellular function. These pathways are responsible for eliciting a physiological response to external stimuli, resulting in biological processes such as cell cycle progression, proliferation, and fate determination. However, the complex networks protein kinases phosphatases that signal regulation difficult study through biochemical genetic methods. traditional techniques fail offer acute spatial temporal resolution optical control provides. To...