A5069205644- Microbial Community Ecology and Physiology
- Metalloenzymes and iron-sulfur proteins
- Plant nutrient uptake and metabolism
- Protist diversity and phylogeny
- Amino Acid Enzymes and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Ammonia Synthesis and Nitrogen Reduction
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Advanced Data Storage Technologies
- Wastewater Treatment and Nitrogen Removal
- Fungal and yeast genetics research
- Marine and coastal ecosystems
- Enzyme Structure and Function
- Polar Research and Ecology
- Legume Nitrogen Fixing Symbiosis
Ginkgo BioWorks (United States)
2022
Michigan State University
2005-2013
Michigan Technological University
2004-2006
University of Potsdam
2005
Nitrate Elimination Company (United States)
2004
Linde (United States)
2004
Hot, Toxic Eukaryote Unusually, the single-celled eukaryote red alga, Galdieria sulphuraria , can thrive in hot, acidic springs. This organism is endowed with extraordinary metabolic talents and consume a variety of strange carbohydrates, as well turn on photosynthesis when food runs out. Schönknecht et al. (p. 1207 ; see Perspective by Rocha ) discerned from phylogenetic analysis its genome that during evolution, G. appears to have commandeered at least 75 bacterial archaeal genes...
Abstract Unicellular algae serve as models for the study and discovery of metabolic pathways, functional dissection cell biological processes such organellar division motility, identification novel genes gene functions. The recent completion several algal genome sequences expressed sequence tag collections establishment nuclear transformation methods has opened way genomics approaches using model systems. thermo-acidophilic unicellular red alga Galdieria sulphuraria represents a particularly...
Nitrate assimilation in autotrophs provides most of the reduced nitrogen on earth. In eukaryotes, reduction nitrate to nitrite is catalyzed by molybdenum-containing NAD(P)H:nitrate reductase (NR; EC 1.7.1.1-3). addition molybdenum center, NR contains iron-heme and flavin adenine dinucleotide as redox cofactors involved an internal electron transport chain from NAD(P)H nitrate. Recombinant, catalytically active Pichia angusta nitrate-reducing, fragment (NR-Mo) was expressed P. pastoris...
Nonphotosynthetic plant metabolic processes are powered by respiratory energy, a limited resource that engineers—like plants themselves—must manage prudently.
Rate-limiting processes of catalysis by eukaryotic molybdenum-containing nitrate reductase (NaR, EC 1.7.1.1-3) were investigated using two viscosogens (glycerol and sucrose) observing their impact on NAD(P)H:NaR activity corn leaf NaR recombinant Arabidopsis yeast NaR. Holo-NaR has "hinge" sequences between stably folded regions housing its internal electron carriers: 1) Hinge 1 the reducing module cytochrome b domain containing heme 2) 2 (CbR) FAD. Solution viscosity negatively impacted...
Biodiesel is emerging as a major renewable energy resource. Glycerol byproduct of biodiesel production, which cannot be directly used fuel. Using <i>Pichia pastoris</i> aerobic yeast cell factory, we engineered pathway to convert glycerol propylene glycol (PG; 1,2-propanediol, 1,2-dihydroxypropane), fuel additive and commodity compound. PG biosynthetic was designed with unique combination 3 enzymes: <i>Escherichia coli</i> methylglyoxal synthase alcohol dehydrogenase; ofunaensis </i>glycerol...
Extremophilic organisms dwell in environments that are characterized by high or low temperatures (thermophiles psychrophiles), very pH-values (acidophiles alkalophiles), salt concentrations (halophiles), pressure (barophiles), extreme drought (xerophiles). Many extremophiles microbes, and many also belong to the prokaryota. <i>Galdieria sulphuraria</i>, however, is a member of group extremophilic eukaryotes named Cyanidiales. Cyanidiales unicellular red micro-algae occur worldwide hot acidic...