A5091853736- Genomics and Phylogenetic Studies
- Evolution and Genetic Dynamics
- Protist diversity and phylogeny
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Clostridium difficile and Clostridium perfringens research
- Environmental DNA in Biodiversity Studies
- Insect and Pesticide Research
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Bacterial Genetics and Biotechnology
- Antibiotics Pharmacokinetics and Efficacy
- Genetic factors in colorectal cancer
- Antibiotic Resistance in Bacteria
- Cancer-related gene regulation
- Neurotransmitter Receptor Influence on Behavior
- Genetic diversity and population structure
- Mycobacterium research and diagnosis
- Research on Leishmaniasis Studies
- Microscopic Colitis
- DNA Repair Mechanisms
- Orthoptera Research and Taxonomy
- Chemical Reactions and Isotopes
- Endodontics and Root Canal Treatments
- Traumatic Brain Injury Research
Hacettepe University
2014-2025
Indiana University Bloomington
2016-2021
Deinococcus bacteria are extremely resistant to radiation, oxidation, and desiccation. Resilience these factors has been suggested be due enhanced damage prevention repair mechanisms, as well highly efficient antioxidant protection systems. Here, using mutation-accumulation experiments, we find that the GC-rich radiodurans an overall background genomic mutation rate similar of E. coli, but differs in spectrum, with A/T G/C (based on a total count 88 A:T→G:C transitions 82 A:T→C:G...
Mycobacterium smegmatis is a bacterium that naturally devoid of known postreplicative DNA mismatch repair (MMR) homologs, mutS and mutL, providing an opportunity to investigate how the mutation rate spectrum has evolved in absence highly conserved primary pathway. Mutation accumulation experiments M. yielded base-substitution 5.27 × 10(-10) per site generation, or 0.0036 genome which surprisingly similar MMR-functional unicellular organisms. Transitions were found more frequently than...
The aim of this study was to examine the effect both promoter and intron polymorphisms serotonin transporter (5HTT) gene on posttraumatic stress disorder (PTSD) development. For purpose, two 5-HTT gene, which are found in (5-HTT gene-linked polymorphic region) second (variable number tandem repeats) were analyzed 100 patients who admitted Emergency Department after a mild physical trauma. None studied have an PTSD development injury, but having L allele for region may cause milder...
The object of this study was to determine the genetic structures three vector species, Phlebotomus tobbi, papatasi, and sergenti, in Cukurova Region Turkey, an endemic focus cutaneous leishmaniasis. diversity indices, neutrality tests hierarchical analysis molecular variance (AMOVA) were performed using partial sequences ITS2 cytochrome b gene regions. In all within population variation higher than between for region. Fst values low non-significant P. papatasi tobbi indicating a weak...
Localized up-down altitudinal shifts and subsequent isolation–admixture of montane species in response to glacial cycles has been proposed as a mechanism for the high diversity along Anatolian mountains. However, specific predictions (the elevation shift model) have yet be tested. Here, we provide first assessment this model promoting inter- intraspecific genetic bush-cricket genus Phonochorion endemic West Lesser Caucasus hotspot. Mitochondrial genes were analysed by Bayesian Markov Chain...
Abstract Organisms adapted to life in extreme habitats (extremophiles) can further our understanding of the mechanisms genetic stability, particularly replication and repair. Despite harsh environmental conditions they endure, these extremophiles represent a great deal Earth’s biodiversity. Here, for first time member archaeal domain, we report genome-wide assay spontaneous mutations halophilic species Haloferax volcanii using direct unbiased method: mutation accumulation experiments...
Abstract We describe the rate and spectrum of spontaneous mutations for social amoeba Dictyostelium discoideum, a key model organism in molecular, cellular, evolutionary developmental biology. Whole-genome sequencing 37 mutation accumulation lines D. discoideum after an average 1,500 cell divisions yields base-substitution 2.47 × 10−11 per site generation, substantially lower than that most eukaryotic prokaryotic organisms, same order magnitude as ciliates Paramecium tetraurelia Tetrahymena...
To investigate the association between VNTR polymorphisms of DRD4, DAT1 and 5-HTT genes obesity.Peripheral blood samples 234 obese (BMI ≥ 30) 148 healthy individuals ≤ 25) were objected to PCR detect 2nd intron 5-HTT, 3rd exon DRD4 3'UTR genes.The obesity genotype distributions allele frequencies tested by Chi Square (χ(2)) test not found statistically significant. BMI values for morbidly > 40) analyzed Kruskal-Wallis significant differences most frequent genotypes genes.As a conclusion,...
Cancer is a consequence of the disruption cellular regulation. Epigenetic one reasons this disruption. factors play role in carcinogenesis by affecting proto-oncogenes and tumor suppressor genes it most popular research areas recent years. DNA methylation, which an epigenetic mechanism, occurs early stages tumorigenesis. Promoter methylation causes silence have been studied extensively various types. The aim study was to investigate promoter certain genes, Cyclin-dependent kinase inhibitor...
The serotonin (5-hydroxytryptamine) transporter (5HTt) gene has been reported to be associated with suicidal behavior. In this study, we have investigated the 5' promoter region (i.e., 5HTt gene-linked polymorphic [5-HTTLPR]) and a 17-base pair variable number of tandem repeats polymorphism in for potential association behavior Turkish population. Genotypes were determined 182 subjects suicide (86 attempted 96 completed suicide) 181 healthy control subjects. Our results showed that allele...
<title>Abstract</title> Environmental conditions, especially diet, affect the diversity of gut microbiota (GM). This within and between populations may influence host’s health fitness, therefore plays important roles in adaptation. Regarding this, we collected fecal samples from natural, rural, suburban, urban habitats to reveal interaction diet compositional functional GM a generalist carnivore, red fox. The prokaryotic was investigated by sequencing 16S rRNA gene V3-V4 regions. 46 archaeal...
Objective: Microorganisms play an important role in all ecosystem processes and a growing body of molecular ecological evidence has shown that microbial biodiversity is much more diverse complex than previously anticipated. This study aimed to determine the prokaryotic microorganisms present Gökçeada Salt Lake Lagoon. Materials Methods: A metabarcoding approach was used diversity Results: 16S rDNA targeted sequencing revealed 5 Archaea 31 Bacteria species represented 63.2%. The most frequent...
Mikroskopi ve kültür tekniklerine dayanan geleneksel mikrobiyal yöntemler oldukça etkili olmalarına rağmen, çevresel örneklerde bulunan türlerin yüksek çeşitliliğini tanımlamakta zaman yetersiz kalmaktadır. Geçtiğimiz son yirmi yılda, moleküler teknikler önemli düzeyde gelişmiştir genomik yaklaşımlar mikroorganizmaların dağılımını daha kapsamlı nicel olarak tanımlamak için kullanılmaktadır. Bu pilot çalışmada, Tuz Gölü'nde prokaryotik ökaryotik çeşitliliği metabarkodlama yaklaşımıyla...
The goal of this study was to identify the optimal marker or combinations for detection gastrointestinal malignancies using reverse-transcriptase polymerase chain (RT-PCR) reaction. To detect presence tumors, we analyzed mucin 1 (MUC1), cytokeratin 19 (CK19), 20 (CK20), and human telomerase reverse transcriptase (hTERT) mRNA in peripheral blood 31 patients with (esophagus, stomach, colorectal) cancer 30 healthy individuals. In RT-PCR analysis blood, 77.4% (24/31), 61.29% (19/31), 45.16%...
Amaç: Hastalık etkeni bakterilerin antibiyotiklere karşı direnç kazanması önemli bir halk sağlığı sorunudur
Abstract Archaea are a major part of Earth’s microbiota and extremely diverse. Yet, we know very little about the process mutation that drives such diversification. To expand beyond previous work with moderate halophilic archaeal species Haloferax volcanii, performed mutation-accumulation experiment followed by whole-genome sequencing in archaeon Halobacterium salinarum. Although Hfx. volcanii Hbt. salinarum have different salt requirements, both highly polyploid genomes similar GC content....
Abstract Analyses of spontaneous mutation have shown that total genome‐wide rates are quantitatively similar for most prokaryotic organisms. However, this view is mainly based on organisms grow best around neutral pH values (6.0–8.0). In particular, the whole‐genome rate has not been determined an acidophilic organism. Here, we in Acidobacterium capsulatum using a direct and unbiased method: mutation‐accumulation experiment followed by sequencing. Evaluation 69 accumulation lines A . after...