A5067104337- RNA Research and Splicing
- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Osteoarthritis Treatment and Mechanisms
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Genetic Neurodegenerative Diseases
- vaccines and immunoinformatics approaches
- Chromosomal and Genetic Variations
- Cardiomyopathy and Myosin Studies
- Cell Adhesion Molecules Research
- Salivary Gland Disorders and Functions
- Molecular Biology Techniques and Applications
- Cleft Lip and Palate Research
- Nuclear Structure and Function
- Periodontal Regeneration and Treatments
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Craniofacial Disorders and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Neurogenetic and Muscular Disorders Research
- Traumatic Brain Injury Research
- T-cell and Retrovirus Studies
- Proteoglycans and glycosaminoglycans research
- Genomics and Chromatin Dynamics
Leiden University Medical Center
2025
Leiden University
2023-2024
Ferdowsi University of Mashhad
2013-2020
<title>Abstract</title> Disease-associated RNA binding protein (RBP) aggregation is a hallmark of several age-related neurodegenerative diseases. How insoluble RBP aggregates leads to cellular dysfunction poorly understood. Here, we investigated the molecular mechanisms affected by PABPN1 aggregates. are nuclear, but regulates nuclear export mRNA. To explore consequences aggregates, performed sequencing and proteomic studies in subcellular fractions an inducible human muscle cell model....
<title>Abstract</title> The accumulation of intracellular protein aggregates is a hallmark aging. In hereditary adult-onset neuromuscular diseases (NMDs), these are not only characteristic but also pathogenic, marking age-related disorders. transition from age-associated non-pathogenic to disease-driving pathogenic remains poorly understood. Poly(A) binding nuclear 1 (PABPN1) forms in post-mitotic aged cells. However, short trinucleotide expansion PABPN1 leads muscle dysfunction...
With the increasing discovery of long noncoding RNAs (lncRNAs), application functional techniques that could have very specific, efficient, and robust effects readouts is necessary. Here, we applied analyzed three gene knockout (KO) strategies to ablate CCAT1 in different colorectal adenocarcinoma cell lines. We refer these as "CRISPR excision", HDR", du-HITI".In order obstruct transcription lncRNA or alter its structure, either a significant segment removed, termination signal inserted...
Alternative polyadenylation (APA) at the 3' UTR of transcripts contributes to cell transcriptome. APA is suppressed by nuclear RNA-binding protein PABPN1. Aging-associated reduced PABPN1 levels in skeletal muscles lead muscle wasting. Muscle weakness oculopharyngeal muscular dystrophy (OPMD) caused short alanine expansion exon1. The expanded forms aggregates, an OPMD hallmark. Whether affects and how it pathology unresolved. To investigate these questions, we developed a procedure including...
Long noncoding RNAs (lncRNAs) are involved in different pathogenesis pathways including cancer pathogenesis. The adenoma-carcinoma pathway colorectal may involve the aberrant and variable gene expression of regulatory RNAs. This study was conducted to analyse prognosis prediction ability two natural antisense transcripts, protein kinase C theta RNA 1 (PRKCQ-AS1), special AT-rich sequence binding (SATB1-AS1) low-grade adenoma, advanced adenocarcinomas.In this study, from RNA-seq analyses...
Abstract Background The methylation of the CpG islands LINE-1 promoter is a tight control mechanism on function mobile elements. However, simultaneous quantification and transcription has not been performed in progressive stages colorectal cancer. In addition, insertion elements genome advanced adenoma stage, precancerous stage before carcinoma emphasized. this study, we quantify transcripts three non-advanced adenoma, adenocarcinoma. analyze LINE-1, Alu, SVA patient tumors with adenomas....
Abstract Ageing has a major adverse impact on maintaining cellular proteostasis and age-related dysregulation leads to an increase in protein aggregation. Equivalently, the accumulation of aggregated proteins accelerates impairment. Accumulation aggregates impaired are hallmarks ageing-associated neuromuscular disorders tissue degeneration is predominantly post-mitotic muscle neuronal cells. A short alanine expansion mutation Poly(A) binding nuclear 1 (PABPN1) causes Oculopharyngeal muscular...
This study was designed to evaluate the in vitro chondrogenic differentiation potential of equine adipose tissue-derived mesenchymal stem cells (AT-MSCs) response different culture conditions. Fat tissue after collection were cultured optimized conditions until passage 3 (P3). P3 as micropellets under for 21 days 5 groups: basic medium a control, (BCM), BCM supplemented with transforming growth factor beta (TGF-β3; 10 ng/mL), bone morphogenetic protein 6 (BMP-6; and both TGF-β3 BMP-6. The...
Abstract Recent discoveries have shown the presence of RNA molecules on cell surface, defying traditional view that only functions intracellularly. However, it is not well understood how cell-surface (csRNA) stably present plasma membrane and what performs surface. By exploiting RNA-sensing ability TLR7 as a specific recombinant probe to detect csRNA coupling with genome-wide CRISPR-Cas9-knockout screening identify genes essential for presentation cells, we identified heparan sulfate (HS)...
The identification of metabolomic biomarkers relies on the analysis large cohorts patients compared to healthy controls followed by validation markers in an independent sample set. Indeed, circulating should be causally linked pathology ensure that changes marker precede disease. However, this approach becomes unfeasible rare diseases due paucity samples, necessitating development new methods for biomarker identification. present study describes a novel combines samples from both mouse...
Abstract Alternative polyadenylation (APA) at the 3’UTR of transcripts contributes to cell transcriptome. APA is suppressed by nuclear RNA binding protein, PABPN1. Aging-associated reduced PABPN1 levels in skeletal muscles lead muscle wasting. Muscle weakness oculopharyngeal muscular dystrophy (OPMD) caused short alanine expansion exon1. The expanded forms aggregates, an OPMD hallmark. Whether affect and how pathology unresolved. To investigate these questions, we developed a procedure...
Abstract Background: LINE-1, Alu, and SVA elements are non-LTR retrotransposons that create approximately one-third of the human genome. The loss tight control mechanisms on function mobile has been implicated in many diseases. methylation CpG islands LINE-1 promoter is one these mechanisms. In this study, we determined expression three stages colorectal non-advanced adenoma, advanced adenocarcinoma. addition, analyzed insertion genome adenomas. Results: We found hypomethylation index...