Columns packed with microsized titanium dioxide particles have been used effectively as precolumns for enriching phosphopeptides from complex mixtures. Nanosized a higher specific surface area and, hence, potentially trapping capacities toward than do particles. Thus, in this study, we employed TiO2-coated magnetic (Fe3O4/TiO2 core/shell) nanoparticles to selectively concentrate protein digest products. Because of their properties, the Fe3O4/TiO2 core/shell that are conjugated target...

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides a straightforward means to differentiate microorganism species based on spectral fingerprinting. The pathogen cell concentration in an infected sample, however, is generally lower than that capable of being detected directly by MALDI-MS. Furthermore, the presence proteins or metabolites biological fluids always causes unavoidable interference for identification species. Vancomycin, which binds D-Ala-D-Ala...

Aims: Our aim was to demonstrate that functional gold nanoparticles can be used as photothermal agents for the selective killing of pathogenic bacteria. Materials & methods: Gold with polygonal shapes, capable absorbing near infrared (NIR) light, were generated through a photochemical reaction. Vancomycin, which bind terminal D-Ala-D-Ala moieties peptide units pathogen cell walls, immobilized on surface nanoparticles. The vancomycin-bound inhibition bacteria growth, under irradiation NIR...

Our aim was to demonstrate that lysozyme-directed generation of gold nanoclusters (Au NCs) are potential antimicrobial agents for antibiotic-resistant bacteria and broad labeling pathogenic bacteria.Lysozyme is an enzyme capable hydrolyzing the cell walls bacteria. In this study, we demonstrated functional Au NCs by using lysozyme as sequester reducing agent precursors at 40 degrees C. addition, shorten reaction time, conducted under microwave irradiation within a short period time first...

Antibiotic-resistant bacteria have emerged because of the prevalent use antibacterial agents. Thus, new agents and therapeutics that can treat bacterial infections are necessary. Vancomycin is a potent antibiotic. Unfortunately, some strains developed their resistance toward vancomycin. Nevertheless, it has been demonstrated vancomycin-immobilized nanoparticles (NPs) capable to be used in inhibition cell growth vancomycin-resistant through multivalent interactions. However, multistep...

Pathogenic bacteria cause the deaths of millions people every year. With development antibiotics, hundreds and thousands people's lives have been saved. Nevertheless, can develop resistance to rendering them insensitive antibiotics over time. Peptides containing specific amino acids be used as antibacterial agents; however, they easily degraded by proteases in vivo. To address these issues, branched peptide dendrimers are now being considered good agents due their high efficacy, protease...

In this paper, we report a method for fabricating biofunctionalized nanoparticles by attaching human immunoglobulin (IgG) onto their surfaces through either electrostatic interactions or covalent binding. We found that these IgG-presenting can bind selectively to the cell walls of pathogens contain IgG-binding sites based on investigation transmission electron microscopy images. Our results demonstrate such Au−IgG may serve as useful nanoscale probes exploring between IgG and pathogens....

We herein demonstrate superparamagnetic Fe3O4 nanoparticles coated with nitrilotriacetic acid derivative (NTA) that can bind different immobilized metal ions are capable of probing diverse target species. Immobilized Ni(II) on the surfaces NTA−magnetic have capability selectively trapping histidine (His)-tagged proteins such as a mutated streptopain tagged 6× His, i.e., C192S (MW ∼42 kDa), from cell lysates. Enrichment was achieved by vigorously mixing sample solution and pipetting in out...

In this study, we used nanocomposite magnetic particles coated with alumina as the affinity probes to selectively concentrate phosphorylated peptides and proteins from a low volume of sample solution. Tryptic digest products phosphoproteins including α- β-caseins, human protein phosphatase inhibitor 1, nonfat milk, egg white, cell lysate were samples demonstrate feasibility approach. only 30 90 s, phosphopeptides sufficient for characterization by MALDI-MS enriched particles, respectively....

Iron oxide nanocomposites of magnetic particles coated with zirconia were used as affinity probes to selectively concentrate phosphopeptides from tryptic digests α- and β-caseins, milk, egg white exemplify the enrichment complex samples. Phosphopeptides, in quantities sufficient for characterization by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), enriched within only 30 s. The probe−target species conjugates separated sample solution simply applying an external...

Escherichia coli and Staphylococcus saprophyticus are the most common causes of urinary tract infections, with 80% these infections caused by uropathogenic E. coli. Because P fimbriae have specificity toward Gal(α1−4)Galβ units, pigeon ovalbumin (POA), whose structure contains terminal moieties, was used as a probe for interaction fimbriated The functional affinity probes bacteria immobilizing POA—a phosphoprotein—onto surface magnetic iron oxide nanoparticles (NPs) coated alumina...

A straightforward method for the rapid detection of presence glutathione S-transferase (GST)-tagged proteins from sample solutions using (GSH)-bound gold nanoclusters (Au@GSH NCs) with luminescence properties as probes by simple observation naked eye was proposed in this study.

Glutathione-bound gold nanoclusters (AuNCs@GSH) can emit reddish photoluminescence under illumination of ultraviolet light. The luminescence the AuNCs@GSH is quenched when chelating with iron ions (AuNCs@GSH-Fe3+), presumably resulting from effective electron transfer between and ions. Nevertheless, we found that be restored in presence phosphate-containing molecules, which suggested possibility using AuNCs@GSH-Fe3+ complexes as selective luminescent switches for metabolites....

Staphylococcus aureus is one of the common pathogenic bacteria responsible for bacterial infectious diseases and food poisoning. This study presents an analytical method based on affinity nanoprobe-based mass spectrometry that enables detection S. in aqueous samples. A peptide aptamer DVFLGDVFLGDEC (DD) can recognize methicillin-resistant (MRSA) was used as reducing agent protective group to generate DD-immobilized gold nanoparticles (AuNPs@DD) from one-pot reactions. The thiol cysteine...

The extensive use of vancomycin has given rise to vancomycin-resistant bacterial strains, such as Enterococci (VRE). We aim explore potent medical treatments that can inhibit the growth VRE.Vancomycin-immobilized gold nanoparticles (Au@Van NPs) with polygonal shapes from one-pot reactions were generated within approximately 7 min.The as-prepared Au NPs exhibit not only antibacterial capability but also photothermal competence. temperature sample solution containing Au@Van be raised by 15°C...

MALDI mass spectrometry is used widely in various fields because it has the characteristics of speed, ease use, high sensitivity, and wide detectable range, but suppression effects between analyte molecules interference from sample matrix frequently arise during analysis. The can be avoided if target species are isolated complicated solutions advance. Herein, we proposed a novel method for achieving such goal. We describe strategy that uses gold nanoparticles to capture charged solution....

The intact fungal spores of several strains four Aspergillus species, flavus, A. oryzae, parasiticus, and sojae, were directly analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. Very simple MALDI spectra are obtained mixing with a matrix such as α-cyano-4-hydroxycinnamic acid or sinapinic acid. from the ablation cell walls owing to acidity solution. results show that aflatoxigenic non-aflatoxigenic have different peak profiles. Furthermore,...

This paper describes a matrix-free method for performing desorption/ionization directly from mesoporous nanocrystalline titania sol-gel thin films, which have good absorption capacity in the ultraviolet (UV) range and can act as assisting materials during UV matrix-assisted laser mass spectrometric (MALDI-MS) analysis. A high concentration of citrate buffer was added into this system to provide proton source reduce presence alkali cation adducts analytes. The analyte signals appear uniformly...

In this study, we demonstrated that microwave-assisted enzymatic digestion could be greatly accelerated by multifunctional magnetite beads. The acceleration of the presence beads was attributable to several features Their capacity absorb microwave radiation leads rapid heating Furthermore, their negatively charged functionalities cause adsorption proteins with opposite charges onto surfaces electrostatic interactions, leading a concentration on present in trace amounts solution. adsorbed are...