A5015410981- Barrier Structure and Function Studies
- Cancer-related molecular mechanisms research
- Axon Guidance and Neuronal Signaling
- Immune cells in cancer
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Immune Response and Inflammation
University of Wisconsin–Madison
2022-2025
The central nervous system (CNS) requires specialized blood vessels to support neural function within specific microenvironments. During neurovascular development, endothelial Wnt/β-catenin signaling is required for BBB development the brain parenchyma, whereas fenestrated that lack properties do not require signaling. Here, we used zebrafish further characterize this phenotypic heterogeneity of CNS vasculature. Using transgenic reporters transcriptional activity, found an inverse...
During neuroinflammation, the proinflammatory cytokine interleukin-1β (IL-1β) impacts blood-brain barrier (BBB) function by disrupting brain endothelial tight junctions, promoting vascular permeability, and increasing transmigration of immune cells. Here, we examined effects Il-1β on
During neurovascular development, brain endothelial cells (BECs) respond to secreted signals from the neuroectoderm that regulate CNS angiogenesis, formation of new blood vessels in brain, and barriergenesis, acquisition blood-brain barrier (BBB) properties. Wnt/β-catenin signaling Vegf are both required for angiogenesis; however, relationship between these pathways is not understood. Furthermore, while essential role this vital process remains unknown. Here, we provide first direct...
Interleukin-1β (IL-1β) is a potent proinflammatory cytokine that plays vital role in the innate immune system. To observe response vivo, several transgenic zebrafish lines have been developed to model IL-1β-induced inflammation and visualize cell migration proliferation real time. However, our understanding of IL-1β limited due an incomplete genome annotation lack functional data for receptors involved inflammatory process. Here, we use combination database mining, genetic analyses, assays...
Summary During neuroinflammation, the proinflammatory cytokine Interleukin-1β (IL-1β) impacts blood-brain barrier (BBB) function by disrupting brain endothelial tight junctions, promoting vascular permeability, and increasing transmigration of immune cells. Here, we examined effects Il-1β on in vivo development BBB. We generated a doxycycline-inducible transgenic zebrafish model that drives secretion CNS. To validate utility our model, showed dose-dependent mortality, recruitment...