A5025262411- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Neonatal Respiratory Health Research
- Cancer Cells and Metastasis
- Congenital Diaphragmatic Hernia Studies
- Mesenchymal stem cell research
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Pregnancy and preeclampsia studies
- Congenital heart defects research
- TGF-β signaling in diseases
- Digital Economy and Work Transformation
- Cancer Research and Treatments
- Gynecological conditions and treatments
- Digestive system and related health
- Health Systems, Economic Evaluations, Quality of Life
- Assisted Reproductive Technology and Twin Pregnancy
- Digital Transformation in Industry
- Medical Imaging and Pathology Studies
- Reproductive Biology and Fertility
- Scheduling and Optimization Algorithms
- Pluripotent Stem Cells Research
- Preterm Birth and Chorioamnionitis
- Healthcare professionals’ stress and burnout
Monash University
2005-2024
Hudson Institute of Medical Research
2019-2022
Walter and Eliza Hall Institute of Medical Research
2011-2021
The University of Melbourne
2011-2015
The Royal Melbourne Hospital
2012
Royal Children's Hospital
2010-2011
Monash Institute of Medical Research
2010
University of British Columbia
1995
Does natural variation exist in the endometrial stem/progenitor cell and protein composition of menstrual fluid across cycles women?Limited exists percentage some types abundance selected proteins within between a cohort women.Menstrual is readily available biofluid that can represent environment, containing cells factors. It unknown whether there cellular content individual women, which has significant implications for use research clinical applications.Menstrual was collected from 11...
Endometrial organoids (EMO) are an important tool for gynecological research but have been limited by generation from (1) invasively acquired tissues and thus advanced disease states (2) women who not taking hormones, excluding 50% of the female reproductive-aged population. We sought to overcome these limitations generating menstrual fluid (MF; MFO) using a method that enables concurrent isolation supernatant, stromal cells, leukocytes biopsies hysterectomy samples hormonal medication...
Growth and development of the fetal lungs is critically dependent on degree to which are expanded by liquid; increases in lung expansion accelerate growth, whereas reductions cause growth cease. The mechanisms mediating expansion-induced unknown but likely include alterations expression genes that regulate cell proliferation. Our aim was isolate identify up- or downregulated increased expansion. In chronically catheterized sheep at 126 days gestational age (GA), left for 36 h, while right...
Endometrial mesenchymal stem cells (eMSC) drive the extraordinary regenerative capacity of human endometrium. Clinical application eMSC for therapeutic purposes is hampered by spontaneous differentiation and cellular senescence upon large-scale expansion in vitro. A83-01, a selective transforming growth factor-β receptor (TGFβ-R) inhibitor, promotes culture blocking senescence, but underlying mechanisms are incompletely understood. In this study, we combined RNA-seq ATAC-seq to study impact...
The degree of fetal lung expansion is a critical determinant growth and alveolar epithelial cell (AEC) differentiation, although the mechanisms involved are unknown. As VDUP1 (vitamin D 3 -upregulated protein 1) can modulate proliferation, induce highly expressed in lung, we have investigated effects on expression its relationship to expansion-induced AEC differentiation sheep. Alterations caused profound changes mRNA levels tissue. Increased significantly reduced from 100 ± 8% control...
Although bronchopulmonary dysplasia is closely associated with an arrest of alveolar development and pulmonary capillary dysplasia, it unknown whether these two features are causally related. To investigate the relationship between capillaries formation, we partially embolized bed. Partial embolization (PPE) was induced in chronically catheterized fetal sheep by injection microspheres into left artery for 1 day (1d PPE; 115d gestational age; GA) or 5 days (5d 110-115d GA). Control fetuses...
Unrestricted somatic stem cells (USSC) derived from umbilical cord blood are an attractive alternative to human embryonic (hESC) for cellular therapy. USSC capable of forming representative all three germ line layers. The aim this study was determine the potential form definitive endoderm following induction with Activin A, a protein known specify formation hESC.USSC were cultured (1) days or without 100 ng/ml A in either serum-free, low-serum serum-containing media, (2) combination 10 FGF4...
Introduction Current research aimed at understanding and preventing stillbirth focuses almost exclusively on the role of placenta. The underlying origins poor placental function leading to stillbirth, however, remain poorly understood. There is evidence demonstrating that endometrial environment in which embryo implants impacts not only establishment pregnancy but also development some outcomes. Menstrual fluid has recently been applied study menstrual disorders such as heavy bleeding or...
The lung is constantly exposed to environmental challenges and must rapidly respond external insults. Mechanisms involved in the repair of damaged involve expansion different epithelial cells repopulate injured cellular compartment. However, factors regulating cell proliferation following injury remain poorly understood. Here we studied role transcriptional regulator Lmo4 during development, regulation adult damage context oncogenic transformation. To study embryonic tumorigenesis, used...
AIMS--To examine the magnitude of thromboplastin and coagulometer interactions on precision International Normalised Ratio (INR) values when manufacturers9 recommended instrument specific Sensitivity Index (ISI) are adopted for INR calculation. METHODS--The variability obtained from four automated phototopical coagulometers frequently used in North American laboratories was studied. When with five commercial thromboplastins moderate to high sensitivity (ISI 0.92-1.97), 20 prothrombin time...
Development of a branching tree in the embryonic lung is crucial for formation fully mature functional at birth. Sox9+ cells present tip primary endoderm are multipotent responsible branch and elongation. We performed genetic screen murine identified aurora kinase b (Aurkb) as an essential regulator ex vivo. In vivo conditional knockout studies confirmed that Aurkb was required development but not necessary postnatal growth repair adult after injury. Deletion led to stunted retained...
To foster a ‘wellbeing curriculum’ in climate with an increasingly competitive graduate jobs market, we believe it is critical to support undergraduate career development and develop positive peer educator relationships, particularly for non-vocational degree programs. However, these relationships between wellbeing their or peer/educator have not been specifically examined. This study used mixed methods approach examine if poor university engagement (quality of peers...
Abstract Lung cancer is the leading cause of death worldwide. Five-year lung survival only 15% and responsible for more deaths than prostate, colon, pancreas, breast cancers combined. Understanding cellular molecular mechanism at origin will generate great insights better management disease. However, lack cell surface markers to identify isolate early progenitor or stem cells in normal represents a gap knowledge that needs be filled cancers. By macroscopic isolation different morphological...
Abstract Endometrial mesenchymal stem cells (eMSC) drive the extraordinary regenerative capacity of human endometrium. Clinical application eMSC for therapeutic purposes is hampered by spontaneous differentiation and cellular senescence upon large-scale expansion in vitro . A83-01, a selective transforming growth factor-β receptor (TGFβ-R) inhibitor, promotes pharmacological culture blocking senescence, but underlying mechanisms are incompletely understood. In this study, we combined RNA-seq...