A5014110371- Influenza Virus Research Studies
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Inflammasome and immune disorders
- Cancer Genomics and Diagnostics
- Proteoglycans and glycosaminoglycans research
- Respiratory viral infections research
- Cannabis and Cannabinoid Research
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- Wnt/β-catenin signaling in development and cancer
- Vaccine Coverage and Hesitancy
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Cell Image Analysis Techniques
- HIV Research and Treatment
University of Geneva
2020-2024
The University of Melbourne
1989-2022
Victorian Comprehensive Cancer Centre
2022
Influenza A virus (IAV) enters host cells mostly through clathrin-dependent receptor-mediated endocytosis. single bona fide entry receptor protein supporting this mechanism remains elusive. Here we performed proximity ligation of biotin to cell surface proteins in the vicinity attached trimeric hemagglutinin-HRP and characterized biotinylated targets using mass spectrometry. This approach identified transferrin 1 (TfR1) as a candidate protein. Genetic gain-of-function loss-of-function...
Influenza A virus infection activates the NLRP3 inflammasome, a multiprotein signaling complex responsible for proteolytic activation and release of proinflammatory cytokine IL-1β from monocytes macrophages. Some influenza (IAV) strains encode short 90-amino acid peptide (PB1-F2) on an alternative open reading frame segment 2, with immunomodulatory activity. We recently demonstrated that contemporary IAV PB1-F2 inhibits NLRP3, potentially by NEK7-dependent activation. binds to its C-terminal...
The Wnt signaling pathway within host cells regulates infections by several pathogenic bacteria and viruses. Recent studies suggested that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection depends on β-catenin can be inhibited the antileprotic drug clofazimine. Since clofazimine has been identified us as a specific inhibitor of Wnt/β-catenin signaling, these works could indicate potential role in SARS-CoV-2 infection. Here, we show is active pulmonary epithelial cells....
Geno- and phenotypic heterogeneity amongst cancer cell subpopulations are established drivers of treatment resistance tumour recurrence. However, due to the technical difficulty associated with studying such intra-tumoural heterogeneity, this phenomenon is seldom interrogated in conventional culture models. Here, we employ a fluorescent lineage technique termed "optical barcoding" (OBC) perform simultaneous longitudinal tracking spatio-temporal fate 64 patient-derived colorectal subclones....
Together with inactivated influenza vaccines (IIV), live attenuated (LAIV) are an important tool to prevent A virus (IAV) illnesses in patients. LAIVs present the advantages have a needle-free administration and trigger mucosal immune response. LAIV is approved for healthy 2- 49-year old individuals. However, due its replicative nature higher rate of adverse events at-risk populations excluded from benefits this vaccine. Using targeted mutagenesis, we modified nonstructural protein 1...
The WHO declared the official end of SARS-CoV-2 caused public health emergency on May 5th, 2023, after two years in which virus infected approximately 750 Mio individuals causing estimated up to 7 deaths. Likely, will continue evolve human population as a seasonal respiratory pathogen. To now prevent severe infection outcomes vulnerable individuals, effective antivirals are urgently needed complement protection provided by vaccines. enters its host cell via ACE2 mediated membrane fusion,...
Abstract The live attenuated influenza vaccine (LAIV) is approved for intranasal spray application in 2-49 year-old patients with safety concerns limiting its use younger children and immunocompromised patients, mainly from the higher incidence of adverse events possibility uncontrolled replication reversion to a pathogenic strain, respectively. Further attenuation LAIV could generally improve profile, which might come at cost reduced immunogenicity. To solve this dilemma, we took advantage...