A5004002637- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Antibiotic Resistance in Bacteria
- Biochemical and Molecular Research
- Evolution and Genetic Dynamics
- CRISPR and Genetic Engineering
- Berberine and alkaloids research
- RNA modifications and cancer
- Pharmacological Effects of Natural Compounds
- Metabolomics and Mass Spectrometry Studies
- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- Mass Spectrometry Techniques and Applications
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Advanced Proteomics Techniques and Applications
- Quinazolinone synthesis and applications
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
HUN-REN Research Centre for Natural Sciences
2019-2024
Institute of Molecular Life Sciences
2017-2023
Budapest University of Technology and Economics
2018-2023
Hungarian Academy of Sciences
2017-2019
Abstract Cells maintain a fine-tuned, dynamic concentration balance in the pool of deoxyribonucleoside 5′-triphosphates (dNTPs). This is essential for physiological processes including cell cycle control or antiviral defense. Its perturbation results increased mutation frequencies, replication arrest and may promote cancer development. An easily accessible relatively high-throughput method would greatly accelerate exploration diversified consequences dNTP imbalances. The incorporation based,...
dUTPase superfamily enzymes generate dUMP, the obligate precursor for de novo dTTP biosynthesis, from either dUTP (monofunctional dUTPase, Dut) or dCTP (bifunctional deaminase/dUTPase, Dcd:dut). In addition, elimination of by these prevents harmful uracil incorporation into DNA. These two beneficial outcomes have been thought to be related. Here we determined relationship between biosynthesis (dTTP/dCTP balance) and prevention DNA uracilation in a mycobacterial model that encodes both Dut...
The sustained success of Mycobacterium tuberculosis as a pathogen arises from its ability to persist within macrophages for extended periods and limited responsiveness antibiotics. Furthermore, the high incidence resistance few available antituberculosis drugs is significant concern, especially since driving forces emergence drug are not clear. Drug-resistant strains can emerge through de novo mutations, however, mycobacterial mutation rates low. To unravel effects antibiotic pressure on...
Abstract Stimulated by the growing interest in role of dNTP pools physiological and malignant processes, we established dNTPpoolDB, database that offers access to quantitative data on from a wide range species, experimental developmental conditions (https://dntppool.org/). The includes measured absolute or relative cellular levels four canonical building blocks DNA exotic dNTPs, as well. In addition quantity, dNTPpoolDB contains ample information sample source, quantitation methods including...
EDTA is commonly used as an efficient chelator of metal ion enzyme cofactors. It highly soluble, optically inactive and does not interfere with most chemicals in standard buffers making a common choice to generate metal-free conditions for biochemical biophysical investigations. However, the controversy literature on activities achieved using or by other means called our attention putative effect beyond chelation. Here, we show that competes nucleotide binding site hydrolase dUTPase...
Protein inhibitors of key DNA repair enzymes play an important role in deciphering physiological pathways responsible for genome integrity, and may also be exploited biomedical research. The staphylococcal repressor StlSaPIbov1 protein was described to efficient inhibitor dUTPase homologues showing a certain degree species-specificity. In order provide insight into the inhibition mechanism, present study we investigated interaction Escherichia coli dUTPase. Although observed strong these...
Cells maintain a fine-tuned balance of deoxyribonucleoside 5′-triphosphates (dNTPs), crucial factor in preserving genomic integrity. Any alterations the nucleotide pool’s composition or chemical modifications to nucleotides before their incorporation into DNA can lead increased mutation frequency and damage. In addition modification canonical dNTPs, cellular de novo dNTP metabolism pathways also produce noncanonical dNTPs. To keep levels low prevent them from incorporating DNA, these dNTPs...
Pathogenicity islands of
Abstract The sustained success of Mycobacterium tuberculosis as a pathogen arises from its ability to persist within macrophages for extended periods and limited responsiveness antibiotics. Furthermore, the high incidence resistance few available antituberculosis drugs is significant concern, especially since driving forces emergence drug are not clear. Drug-resistant strains can emerge through de novo mutations, however, mycobacterial mutation rates low. To unravel effects antibiotic...
The sustained success of Mycobacterium tuberculosis as a pathogen arises from its ability to persist within macrophages for extended periods and limited responsiveness antibiotics. Furthermore, the high incidence resistance few available antituberculosis drugs is significant concern, especially since driving forces emergence drug are not clear. Drug-resistant strains can emerge through de novo mutations, however, mycobacterial mutation rates low. To unravel molecular mechanisms antibiotic...
The sustained success of Mycobacterium tuberculosis as a pathogen arises from its ability to persist within macrophages for extended periods and limited responsiveness antibiotics. Furthermore, the high incidence resistance few available antituberculosis drugs is significant concern, especially since driving forces emergence drug are not clear. Drug-resistant strains can emerge through de novo mutations, however, mycobacterial mutation rates low. To unravel effects antibiotic pressure on...
Tuberculosis (TB) presents significant medical challenges, largely due to the genetic diversity of
The sustained success of Mycobacterium tuberculosis as a pathogen arises from its ability to persist within macrophages for extended periods and limited responsiveness antibiotics. Furthermore, the high incidence resistance few available antituberculosis drugs is significant concern, especially since driving forces emergence drug are not clear. Drug-resistant strains can emerge through de novo mutations, however, mycobacterial mutation rates low. To unravel effects antibiotic pressure on...