A5084646888- Marine and coastal ecosystems
- Bacterial Genetics and Biotechnology
- Marine Biology and Ecology Research
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Aquatic Ecosystems and Phytoplankton Dynamics
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Isotope Analysis in Ecology
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Algal biology and biofuel production
- Biochemical and Molecular Research
- Diatoms and Algae Research
- Protist diversity and phylogeny
- Peptidase Inhibition and Analysis
- Underwater Acoustics Research
- Microbial Metabolic Engineering and Bioproduction
- Geographic Information Systems Studies
- Marine Toxins and Detection Methods
- Enzyme Production and Characterization
- Seaweed-derived Bioactive Compounds
- Atmospheric and Environmental Gas Dynamics
- Protein Structure and Dynamics
Laboratoire d’Océanologie et de Géosciences
2017-2025
Université de Lille
2021-2025
Centre National de la Recherche Scientifique
2001-2025
Institut de Recherche pour le Développement
2023-2024
Université du littoral côte d'opale
2024
Institut de Biologie Intégrative de la Cellule
2016
Université Paris-Sud
1997-2011
Institut de Biochimie et Biophysique Moléculaire et Cellulaire
2001-2011
Université Paris Cité
1997
Abstract. Long-term monitoring of phytoplankton communities is essential for understanding the functioning and evolution marine systems. This paper presents a decadal dataset on observations conducted along coastal-offshore transect by Strait Dover, at fine spatial resolution, using an automated in vivo approach. Nine stations (∼ 1 km apart) were sampled off Slack estuary, representing northern limit Marine Protected Area “Picard Estuaries Opal Seas” (EPMO). Since 2012, functional groups...
The MraY translocase catalyzes the first membrane step of bacterial cell wall peptidoglycan synthesis (i.e. transfer phospho-N-acetylmuramoyl-pentapeptide motif onto undecaprenyl phosphate carrier lipid), a reversible reaction yielding undecaprenylpyrophosphoryl-N-acetylmuramoyl-pentapeptide (lipid intermediate I). This essential integral protein, which is considered as very promising target for search new antibacterial compounds, has thus far been clearly underexploited due to its intrinsic...
Summary In Escherichia coli many enzymes including MurG are directly involved in the synthesis and assembly of peptidoglycan. is an essential glycosyltransferase catalysing last intracellular step peptidoglycan synthesis. To elucidate its role during elongation division events, localization using immunofluorescence microscopy was performed. exhibited a random distribution cell envelope with relatively higher intensity at site. This mid‐cell dependent on presence mature divisome. Its lateral...
Abstract. Global change is known to exert a considerable impact on marine and coastal ecosystems, affecting various parameters such as sea surface temperature (SST), runoff, circulation patterns the availability of limiting nutrients (like nitrogen, phosphorus silicon), with each influencing phytoplankton communities differently. This study based weekly fortnightly in vivo fine-spatial-resolution (∼ 1 km) observations along an nearshore–offshore gradient French waters Eastern English Channel...
Introduction While crucial to ensuring the production of accurate and high-quality data—and avoid erroneous conclusions—data quality control (QC) in environmental monitoring datasets is still poorly documented. Methods With a focus on annual inter-laboratory comparison (ILC) exercises performed context French coastal SOMLIT network, we share here pragmatic approach QC, which allows calculation systematic random errors, measurement uncertainty, individual performance. After an overview...
The effect of environmental change in structuring the phytoplankton communities coastal waters Eastern English Channel was investigated by applying a trait-based approach on two decades (1996-2019) monitoring diatoms and Phaeocystis . We show that species richness an unbalanced nutrient supply context influenced wind-driven processes, ecological specialization for dissolved inorganic phosphorous, temporal niche differentiation, competition-defense and/or growth-defense trade-off, coexistence...
Although eutrophication induced by anthropogenic nutrient enrichment is a driver of shifts in community composition and eventually threat to marine biodiversity, the causes consequences on ecosystem functioning remain greatly unknown. In this study, applying trait-based approach measuring niche breadth diatoms copepods, drivers underlying mechanisms seasonal species succession these ecological communities coastal system dominated spring Phaeocystis blooms were explored. It suggested that...
ABSTRACT Many species of gram-positive bacteria produce branched peptidoglycan precursors resulting from the transfer various l -amino acids or glycine amino acyl-tRNA to ɛ-amino group -lysine. The UDP-MurNAc-pentapeptide: -alanine ligase and alanyl-tRNA synthetase genes Enterococcus faecalis were identified, cloned, overexpressed in Escherichia coli . purified enzymes necessary sufficient for tRNA-dependent addition UDP-MurNAc-pentapeptide vitro. belonged Fem family proteins, which...
Plasmids for the high‐level overproduction of wild‐type, and C‐ N‐terminal His‐tagged MurG N ‐acetylglucosaminyl transferase from Escherichia coli were constructed. In complementation tests three forms active in vivo. After IPTG induction, growth, spheroplast formation lysis, overproduced proteins mainly present (90%) particulate fraction. Readily solubilized by CHAPS, they purified without any detergent to over 80% purity both but only up 20% wild‐type form. The enzymatic activity each...
Turbulence is one of the least investigated environmental factors impacting ecophysiology phytoplankton, both at community and individual species level. Here, we investigated, for first time, effect a turbulence gradient (Reynolds number, from Reλ = 0 to 360) on two marine diatom Pseudo-nitzschia their associated bacterial communities under laboratory conditions. Cell abundance, domoic acid (DA) production, chain formation, Chl content P. fraudulenta multiseries were higher intermediate (Reλ...
Stable analogs of bacterial transferase MraY substrate or product with a pyrophosphate surrogate in their structure are described. β-ketophosphonates were designed as bioisosteres and investigated UDP-GlcNAc mimics. The developed strategy allows introduction structural diversity at late stage the synthesis. biological activity synthesized compounds was evaluated on enzyme.
Abstract. Global change is known to exert a considerable impact on marine and coastal ecosystems, affecting various parameters such as sea surface temperature, rain-off, circulation patterns, the availability of limiting nutrients like nitrogen, phosphorus silicon, each influencing phytoplankton communities differently. This study based weekly fortnightly in vivo observations French waters Eastern English Channel at fine spatial resolution (∼1 km) along an inshore-offshore gradient Strait...