To determine macrolide–lincosamide–streptogramin (MLS) resistance determinants in the Capnocytophaga genus and to describe prevalence of β-lactam genes human oral species. Forty-eight isolates identified by analysis 16S rRNA sequences were isolated from subgingival samples 14 haematology patients (HPs), 11 periodontitis (PPs) 17 healthy volunteers (HVs). MICs MLS antibiotics obtained for all isolates. blaCfxA, blaCSP-1 (encoding a new class A β-lactamase) [erm(F), erm(B), erm(Q), erm(D),...

The purpose of this study was to conduct phytochemical analysis sea buckthorn pulp oil and evaluate the antimicrobial, anti-biofilm antioxidant activities its mouthwash form.Fatty acid composition determined by GC-MS analysis, which revealed that, mono-unsaturated fatty acid, palmitoleic saturated palmitic were major constituents. antimicrobial capacities form evaluated against Streptococcus gordonii, Porphyromonas gingivalis, Actinomyces viscosus Candida albicans, according European Norms,...

Summary P orphyromonas gingivalis , an important etiological agent of periodontal disease, is frequently found associated with T reponema denticola anaerobic spirochete, in pathogenic biofilms. However, interactions between these two bacteria are not well understood at the molecular level. In this study, we seek to link influence . on expression proteases its capacities adhere and form The genes encoding Arg‐gingipain A ( RgpA ), L ys‐gingipain Kgp hemagglutinin HagA ) were more strongly...

Porphyromonas gingivalis, a gram-negative anaerobic bacterium, is major periopathogen whose transmission from host to involves exposure atmospheric oxygen. P. gingivalis contains genetic factors that function in an oxidative stress response, but their expression has not been analyzed during The aim of this study was obtain better understanding adaptation gingivalis.The aerotolerance wild-type and oxyR mutant strains were determined, quantitative polymerase chain reaction performed analyze...

Periodontitis are bacterial inflammatory diseases, where the biofilms present on tooth-supporting tissues switch from a healthy state towards pathogenic state. Among species involved in disease, Porphyromonas gingivalis has been shown to induce dysbiosis, and virulence of otherwise bacteria like Streptococcus gordonii. During biofilm development, primary colonizers such as S. gordonii first attach surface allow subsequent adhesion periodontal pathogens P. gingivalis. Interactions between...

The purpose of this study was to evaluate various biological effects silver-zeolite and a polyphenol-rich extract A. nodosum (ASCOP) prevent and/or treat biofilm-related oral diseases. Porphyromonas gingivalis Streptococcus gordonii contribute the biofilm formation associated with chronic periodontitis. In study, we evaluated in vitro antibacterial anti-biofilm (Ag-zeolite) combined ASCOP on P. S. growth capacity. We also studied anti-inflammatory antioxidant capacities cell culture models....

Salmonella Heidelberg is one of the most common serovar causing foodborne illnesses. To limit development digestive bacterial infection, food supplements containing probiotic bacteria can be proposed. Commensal non-toxigenic Bacteroides fragilis has recently been suggested as a next-generation candidate. By using an original triple co-culture model including Caco-2 cells (representing human enterocytes), HT29-MTX mucus-secreting goblet cells), and M differentiated from by addition Raji B...

In Brief BACKGROUND: Tramadol is a synthetic analog of codeine with opioid and local anesthetic properties. It used as central-acting analgesic, recently, in subcutaneous or intradermal injections, anesthetic. We investigated vitro the antibacterial activity tramadol absence any anesthetics against Escherichia coli, Staphylococcus aureus, epidermidis, Pseudomonas aeruginosa pathogens that can cause infectious complications after regional anesthesia. METHODS: Bacterial cultures were grown for...

Porphyromonas gingivalis is an anaerobic periodontal pathogen that resides in the complex multispecies microbial biofilm known as dental plaque. Effective reporter tools are increasingly needed to facilitate physiological and pathogenetic studies of biofilm. Fluorescent proteins ideal reporters for conveniently monitoring growth, but restricted by several environmental factors, such a requirement oxygen emit fluorescence. We developed fluorescent plasmid, SNAP-tag, labeling P. cells, which...

Enterotoxigenic Escherichia coli was studied in waste water, river and seawater from six locations along the west coast of Normandy by using polymerase chain reaction (PCR) to amplify heat labile (LT) gene. Cellular DNA extracted centrifugation pellets amplified PCR. The PCR products were detected gel electrophoresis confirmed hybridization assay, an 850 base pair HindIII fragment probe pEWD299 conjugated digoxigenin specific for LT Results amplification compared with those GM1 enzyme-linked...

ABSTRACT A beta-lactamase gene ( cfxA3 , 966 bp) was isolated from a beta-lactam-resistant Capnocytophaga ochracea clinical isolate and amplified using primers the cfxA of Bacteroides vulgatus . The MICs third-generation cephalosporins were much higher than those transconjugant Escherichia coli strain. deduced protein sequence, by comparison with CfxA2 Prevotella intermedia had Y239D substitution possessed characteristics class A, group 2e beta-lactamase.

Introduction. Porphyromonas gingivalis is considered as a major etiological agent in the onset and progression of chronic destructive periodontitis. Porphyromonus fimA type has been correlated to virulence potential strain; therefore this gene could be involved ability P. reach blood stream.Objective. The classifications types will compared subgingival plaque samples collected after scaling root planing periodontitis patients.Materials methods. Fifteen patients requiring were enrolled....

The gut microbiota contributes to human health and disease; however, the mechanisms by which commensal bacteria interact with host are still unclear. To date, a number of in vitro systems have been designed investigate host-microbe interactions. In most intestinal models, enteroendocrine cells, considered as potential link between several diseases, were missing. present study, we generated new model adding cells (ECC) L-type (NCI-H716) one that previously described including enterocytes,...

The aim of this study was to evaluate the prevalence beta-lactamase-producing Capnocytophaga isolates in young children hospitalized Pediatric Oncology Department Hôpital Sud (Rennes, France) over a ten-year period (1993–2002). In neutropenic children, periodic survey oral cavity allows predictive evaluation risk systemic infections by spp. 449 with cancer, 3,053 samples were collected swabbing and plated on TBBP agar. susceptibility five beta-lactams determined. A total 440 strains...

ABSTRACT Randomly amplified polymorphic DNA (RAPD) analysis is a polymorphism assay commonly used for fingerprinting genomes. After optimizing the reaction conditions, samples of Escherichia coli H10407 were assayed to determine influence osmotic and/or oligotrophic stress on variations in RAPD banding patterns. Genetic rearrangements or topology could be detected as changes agarose gel electrophoresis profiles. A new amplicon generated using extracted from bacteria prestarved by an and...