The study and optimization of epoxy-based negative photoresist (SU-8) microstructures through a low-cost process without the need for cleanroom facility is presented in this paper. It demonstrated that Ultraviolet Rays (UV) exposure equipment, commonly used Printed Circuit Board (PCB) industry, can replace more expensive less available as Mask Aligner has been last 15 years SU-8 patterning. Moreover, high transparency masks, printed photomask, are used, instead chromium masks. fabrication...

The advancement of organ-on-a-chip (OoC), as end-use preclinical tool, has been slackened with the technological and engineering challenges that in-situ real-time monitoring physicochemical biological parameters still represent. In this study, envisioned solution to surpass challenge is integration a microneedle (MN) patch into microfluidic device collect cell culture media directly from organ chamber drive it module contain biosensing system for monitoring. For that, porous MNs (PMNs) were...

Biofouling is the major factor that limits long-term monitoring studies with automated optical instruments. Protection of sensing areas, surfaces, and structural housing sensors must be considered to deliver reliable data without need for cleaning or maintenance. In this work, we present design field validation different techniques biofouling protection based on materials, biocides, transparent coatings. Six turbidity probes were built using polylactic acid (PLA), acrylonitrile butadiene...

By combining biomaterials, cell culture, and microfluidic technology, organ-on-a-chip (OoC) platforms have the ability to reproduce physiological microenvironment of human organs. For this reason, these advanced devices been used resemble various diseases investigate novel treatments. In addition experimental assessment, numerical studies biodevices performed aiming at their improvement optimization. Despite considerable progress in modeling biodevices, validation computational models...

Alzheimer’s disease (AD), a progressive neurodegenerative disorder, is marked by the abnormal production of amyloid-beta (Aβ) fibrils, key biomarker for diagnosis and illness monitoring. Advanced microfluidic devices, such as brain-on-a-chip (BoC), are innovative preclinical tools with potential to revolutionize AD early treatment. However, existing BoCs face limitations, including challenges in biosensing integration, limited sensitivity, automation. In this study, we demonstrate...

As organizações, no intuito de serem mais ágeis e competitivas em um mercado com amplos desafios, ajustam suas estruturas para melhor gerir recursos alcançar resultados. Nesse sentido, o presente estudo teve como objetivo geral analisar modelo gestão por projetos adotado pelo Instituto Alpha comparação aos modelos estruturais tradicionais. Tratou-se uma pesquisa descritiva, levantamento caso, na qual foi aplicado questionário adaptado Oliveira, Vetter Conceição (2023), direcionado...

Abstract This study addresses the importance of enhancing traditional fluid-flow solvers by introducing a Machine Learning procedure to model pressure fields computed standard solvers. The conventional approach involves enforcing pressure–velocity coupling through Poisson equation, combining Navier–Stokes and continuity equations. solution this equation constitutes substantial percentage overall computational cost in fluid flow simulations, therefore improving its efficiency can yield...

The manufacture of three-dimensional patterned electroactive poly(vinylidene fluoride-co-chlorotrifluoroethylene) microstructures with tailored architecture, morphology, and wettability is presented. are fabricated using a simple, effective, low cost, reproducible technique based on microfluidic technology. These novel structures can represent innovative platforms for advanced strategies in wide range biotechnological applications, including tissue engineering, drug delivery, microfluidic,...

In an organ-on-a-chip (OoC) device, temperature control is essential for a well-controlled and human representative microenvironment. This work presents the design, numerical simulation, fabrication characterization of three aluminium microheater geometries into OoC device. Two them are circular-based, with different curvature filleting angles line widths, third Hilbert-based geometry. Numerical simulations in COMSOL Multiphysics were performed to evaluate heat distribution power consumption...

Forest conservation efforts through regulatory enforcement routinely failed to prevent large scale deforestation in the Brazilian Amazon. However, a turning point occurred 2005, when combination of unfavorable economic conditions and an unprecedented coordinated effort between governmental institutions resulted gradual slowdown deforestation. The continuation this environment recovery expansion after 2009 suggests that achieved measure success not experienced before. In study, impact fines,...

This paper focuses on a novel and portable device prototype with optical detectors to quickly efficiently detect hemozoin (Hz) in blood, aiming at malaria diagnostics.Taking advantage of the particular features parasite infected particularly Hz formation, main innovation described is for quantification parasitic through absorbance spectrophotometry. comprises detection chambers fluidic samples, an emission system, power supply system provide autonomy. The working principle based colorimetric...

The advanced and widespread use of microfluidic devices, which are usually fabricated in polydimethylsiloxane (PDMS), requires the integration many sensors, always compatible with fabrication processes. Moreover, current limitations existing optical electrochemical oxygen sensors regarding long-term stability due to sensor degradation, biofouling, processes cost have led development new approaches. Thus, this manuscript reports development, characterization a low-cost highly sensitive...

Harmful algal blooms (HABs) are a recurring phenomenon along all continents, posing global threat, particularly due to shellfish poisoning and public health, their timely monitoring is vital for safeguarding the blue economy. The ability efficiently isolate species of interest, such as harmful dinoflagellates, key process HABs which complex because typically high diversity marine microalgae communities. This study introduces simple spiral microchannel device using inertial microfluidics...

This paper presents the design, fabrication and characterization of temperature microsensors based on Resistance Temperature Detectors (RTDs) with a meander-shaped geometry. Numerical simulations were performed for studying sensitivity RTDs according to their windings numbers as well optimizing layout. These fabricated using well-established microfabrication photolithographic techniques. The sensors feature high (0.3542 mV/°C), linearity reproducibility in range 35 45 °C. Additionally, each...

Accurate pH sensing is essential for the effective physiological control of culture media in organ-on-a-chip (OoC) systems. This work proposes a miniaturized optical detection system, based on transmittance, level quantification EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ medium. Firstly, using commercial spectrophotometric setup, set wavelengths (500, 560, and 600 nm) was selected, as these assure distinctive slope variations different levels. Then, current-to-frequency converter,...

This paper reports the fabrication process and characterization of a flexible pressure sensor based on polydimethylsiloxane (PDMS) multi-walled carbon nanotubes (CNT-PDMS). The proposed approach relies patterned CNT-PDMS nanocomposite strain gauges fabricated with SU-8 microstructures (with micropatterns) in low-cost simple process. polymer is mounted over PDMS membrane, which, turn, lies top diaphragm like structure. configuration enables membrane to bend when applied, thereby affecting...