A5061287825- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Genetics, Aging, and Longevity in Model Organisms
- Retinal Development and Disorders
University of California, Berkeley
2020-2024
Innovative Genomics Institute
2021-2024
Viruses and virally derived particles have the intrinsic capacity to deliver molecules cells, but difficulty of readily altering cell-type selectivity has hindered their use for therapeutic delivery. Here, we show that cell surface marker recognition by antibody fragments displayed on membrane-derived encapsulating CRISPR-Cas9 protein guide RNA can genome editing tools specific cells. Compared conventional vectors like adeno-associated virus rely evolved capsid tropisms encoded cargo, these...
Abstract DNA nanostructures are a promising tool to deliver molecular payloads cells. origami structures, where long single-stranded is folded into compact nanostructure, present an attractive approach package genes; however, effective delivery of genetic material cell nuclei has remained critical challenge. Here, we describe the use encoding intact human gene and fluorescent protein as templates for integration by CRISPR-mediated homology-directed repair (HDR). Our design includes...
Genome editing is poised to revolutionize treatment of genetic diseases, but poor understanding and control DNA repair outcomes hinders its therapeutic potential. especially understudied in nondividing cells like neurons, which must withstand decades damage without replicating. This lack knowledge limits the efficiency precision genome clinically relevant cells. To address this, we used induced pluripotent stem (iPSCs) iPSC-derived neurons examine how postmitotic human Cas9-induced damage....
Abstract Viruses and virally-derived particles have the intrinsic capacity to deliver molecules cells, but difficulty of readily altering cell-type selectivity has hindered their use for therapeutic delivery. Here we show that cell surface marker recognition by antibody fragments displayed on membrane-derived encapsulating CRISPR-Cas9 protein guide RNA can target genome editing tools specific cells. These Cas9-packaging enveloped delivery vehicles (Cas9-EDVs), programmed with different...
Abstract DNA nanostructures are a promising tool for delivery of variety molecular payloads to cells. origami structures, where 1000’s bases folded into compact nanostructure, present an attractive approach package genes; however, effective genetic material cell nuclei has remained critical challenge. Here we describe the use encoding intact human gene and fluorescent-protein as templates integration by CRISPR-mediated homology-directed repair (HDR). Our design includes CRISPR-Cas9...