A5058800521- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Vibrio bacteria research studies
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Respiratory viral infections research
- Nanoparticles: synthesis and applications
- Viral gastroenteritis research and epidemiology
- Molecular Biology Techniques and Applications
- Enterobacteriaceae and Cronobacter Research
- Infective Endocarditis Diagnosis and Management
- Prenatal Screening and Diagnostics
- Evolution and Genetic Dynamics
- Burkholderia infections and melioidosis
- Cancer Risks and Factors
- Polysaccharides and Plant Cell Walls
- Vaccine Coverage and Hesitancy
- Escherichia coli research studies
- Virus-based gene therapy research
- Microbial Inactivation Methods
Instituto de Salud Carlos III
2024
Centro de Investigación Biomédica en Red
2024
Centro Nacional de Microbiologia
2024
Hospital Universitario La Paz
2020-2023
Universidad Complutense de Madrid
2015-2018
University of Balamand
2012-2018
In food facilities, biofilms or their debris might act as helpers for attracting free floating microorganisms. this sense, Pseudomonas fluorescens, a dense biofilm producer frequently isolated from contact surfaces, could be good candidate sheltering other microorganisms, such Listeria monocytogenes. The main objective of work was to evaluate the ability L. monocytogenes colonize pre-established biofilms. For this, movement throughout mature green fluorescent protein (GFP) - tagged strain...
Currently, antimicrobial resistance is one of the most prominent public health issues. In fact, there increasing evidence that animals constitute a reservoir resistance. collaboration with Lebanese Ministry Agriculture, aim this study was to determine prevalence intestinal carriage multi-drug-resistant Gram-negative Bacilli in poultry farms at national level. Between August and December 2015, 981 faecal swabs were obtained from 49 distributed across Lebanon. The subcultured on MacConkey agar...
The worldwide increase in the emergence of carbapenem resistant Acinetobacter baumannii calls for investigation into alternative approaches treatment. This study aims to evaluate colistin-carbapenem combinations against spp., order potentially reduce need high concentrations antibiotics therapy. was conducted on 100 non-duplicate isolates that were collected from different patients admitted at Saint George Hospital-University Medical Center Beirut. identified using API 20NE strips, which...
Extended-spectrum beta-lactamases (ESBL) and carbapenemase-producing organisms pose severe problems for hospitalized patients. In the absence of efficient sanitation sewage disposal, risks transmission hospital into community are high. Our objectives were to study occurrence mechanisms resistance multidrug-resistant gram-negative bacilli in two Lebanese treatment plants. Wastewater samples collected, filtered, cultivated on MacConckey+cefotaxime agars. ESBL, AmpC, metallo-β-lactamases (MBL),...
Introduction:Acinetobacter baumannii is a nosocomial pathogen that usually affects critically ill patients. High mortality rates have been associated with MDR A. infections. Carbapenem resistance among these isolates increasing worldwide and certain International Clones (ICs) oxacillinases (OXAs). Moreover, this organism possesses wide range of virulence factors, whose expression not yet fully understood. In study, clinical are characterized in terms antibiotic resistance, mechanisms...
Acinetobacter baumannii is a nosocomial pathogen that showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this study, we aim at characterizing A. isolated from Spanish hospital in terms antimicrobial susceptibility, clonality, carbapenemase genes harbored, and determinants expressed.Fifty nine clinical...
Introduction Carbapenemase-Producing Escherichia coli (CP-Eco) isolates, though less prevalent than other CP-Enterobacterales, have the capacity to rapidly disseminate antibiotic resistance genes (ARGs) and cause serious difficult-to-treat infections. The aim of this study is phenotypically genotypically characterizing CP-Eco isolates collected from Spain better understand their mechanisms population structure. Methods Ninety representative received 2015 2020 25 provinces 59 hospitals...
The dissemination of Multi Drug Resistant Organisms (MDROs) is one the major public health problems addressed nowadays. High fecal carriage rates MDR Enterobacteriaceae were reported from Lebanese nursing homes. Studies have shown that acquisition resistance genes by bacteria might confer a fitness cost detected as decrease in frequency these compared to sensitive isolates. In this study, competitive growth isolated elderly assessed. Sensitive and ESBL-producing Escherichia coli Klebsiella...
We used metagenomics to analyze one sputum sample from a patient with symptoms of respiratory infection that yielded negative results for all pathogens tested. detected two viral genomes could be assembled and showed sequence similarity redondoviruses, recently described group within the CRESS-DNA viruses. One hundred samples were screened presence these viruses using specific primers. was positive same viruses, another them. These findings raise questions about possible role redondoviruses...
Nanoparticles and nanocomposites with low concentration in the body are non-toxic can be suitable alternative to antibiotics. Gold nanoparticles (AuNPs), silver (AgNPs), silver-gold nanocomposite (Ag-Au NCs) were synthesized for first time using galangin (GA) from Suaeda maritima (SM). A simple method was studied optimized biosynthesis of AuNPs, AgNPs, Ag-Au NCs GA SM. variety phytochemical compounds found SM, such as phenols, convert metal into ions serve capping agents. The SM analysis...
Several studies have reported the isolation of resistant Gram-Negative Bacilli in Lebanon. However, those are new and scarce as compared to worldwide data mostly restricted single center studies. In this review, we attempt provide a reliable comprehensive report describing current situation providing prospects for bacterial resistance shown that Extended Spectrum β -Lactamase-producing E. coli K. pneumoniae strains being increasingly reported. Moreover, 2.15% 7.84% isolates carbapenem up 30%...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first detected in Madrid, Spain, on 25 February 2020. It increased frequency very fast and by the end of May more than 70,000 cases had been confirmed reverse transcription-polymerase chain reaction (RT-PCR). To study lineages diversity viral population during this epidemic wave Madrid we sequenced 224 SARS-CoV-2 genomes collected from three hospitals to All known major were found set samples, though B.1 B.1.5 most frequent...
The aim of this study was to determine the usefulness MPC carbapenems against clinical isolates Pseudomonas spp . and Acinetobacter spp. assess its possible relationship with mechanisms resistance. Detection resistance performed using Antibiotic Susceptibility Testing, Double Disk Synergy, disk antagonism, addition NaCl medium, PBA or EDTA Carbapenem disks, Cefoxitin CCCP test for 10 baumannii strains. MIC were determined broth macrodilution plate dilution methods, respectively. Four strains...
Introduction Intestinal colonization by Multi-Drug Resistant Organisms (MDROs) can pose a threat on the health of critically ill patients. The extent these organisms is related to previous antibiotic treatments and their ability cause infections among adult aim this study determine relationship between intestinal Relative Loads (RLs) selected resistance genes, consumption extra-intestinal spread pediatric Methods RLs bla CTX-M-1-Family , OXA-1 OXA-48 VIM were determined in 382 rectal swabs...
This study evaluates the rates of biofilm formation in light different characteristics twelve <italic>A. baumannii</italic> clinical isolates.
The relationship between antibiotic consumption and resistance has been widely evaluated. Pseudomonas aeruginosa is one of the most important opportunistic pathogens in nosocomial setting, its to antibiotics increaseing. Production metallo-β-lactamases (MBLs) currently fearful mechanism due potential dissemination. This study aimed evaluate correlation (expressed DDD/100 bed days) % isolates patients) different time periods for P. 2006 2009 at Saint George Hospital University Medical Center...
The population structure of a set OXA-48-producing Klebsiella pneumoniae isolates belonging to sequence type 11 (ST11 Kp-OXA) and obtained from two hospitals in Madrid the period 2012 2015 was studied by genome sequencing. Overall, 97 ST11 Kp-OXA were sequenced their demography Bayesian phylodynamic analysis using core-genome SNVs. In total, 92 Hospital La Paz, 57 them selected units. remaining five different units Doce de Octubre. Altogether, 96 out could be ascribed single lineage that...
(1) Background: We determined the relevance of intestinal dominance by Serratia spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. was cultured neonates (Group 1), while other 29 culture-negative 2). Total DNA extracted rectal swabs, and quantitative PCRs (qPCRs) using Serratia- 16SrRNA-gene-specific primers performed. relative loads (RLs) ΔΔCt. Clonality investigated random amplified polymorphic analysis whole-genome...
Pectin is a heterogeneous polysaccharide present in plants and citrus fruits. It exhibits different beneficial biological activities. Conflicting reports exist about the antimicrobial effect of pectin its derivatives.In this study, we investigate Modified Citrus (MCP) against Staphylococcus aureus, pathogen showing increasing rates resistance worldwide.Forty-three clinical isolates S. aureus were obtained from hospital North Lebanon. Minimum Inhibitory Concentrations (MICs) Bactericidal...
Objectives To evaluate the performance of oral saliva swab (OSS) reverse transcription PCR (RT-PCR) compared with RT-PCR and antigen rapid diagnostic test (Ag-RDT) on nasopharyngeal swabs (NPS) for SARS-CoV-2 in children. Design Cross-sectional multicentre study. Setting Study nested a prospective, observational cohort (EPICO-AEP) performed between February March 2021 including 10 hospitals Spain. Patients Children from 0 to 18 years symptoms compatible Covid-19 ≤5 days duration were...