A5062408856- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Neuropeptides and Animal Physiology
- Microbial Inactivation Methods
- Peptidase Inhibition and Analysis
- Protein Structure and Dynamics
- COVID-19 Clinical Research Studies
- Neuroscience and Neuropharmacology Research
- SARS-CoV-2 and COVID-19 Research
- Nicotinic Acetylcholine Receptors Study
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
- Long-Term Effects of COVID-19
- Ultrasound and Hyperthermia Applications
National Institute of Chemistry
2020-2024
University of Ljubljana
2021-2022
Abstract The regulation of protein function by external or internal signals is one the key features living organisms. ability to directly control a selected would represent valuable tool for regulating biological processes. Here, we present generally applicable proteins called INSRTR, based on inserting peptide into loop target that retains its function. We demonstrate versatility and robustness coiled-coil-mediated regulation, which enables designs either inactivation activation functions,...
The SARS-CoV-2 pandemic imposed a large burden on health and society. Therapeutics targeting different components processes of the viral infection replication cycle are being investigated, particularly to repurpose already approved drugs. Spike protein is an important target for both vaccines therapeutics. Insights into mechanisms spike-ACE2 binding cell fusion could support identification compounds with inhibitory effects. Here, we demonstrate that integrity disulfide bonds within...
Like other pentameric ligand-gated channels, glycine receptors (GlyRs) contain long intracellular domains (ICDs) between transmembrane helices 3 and 4. Structurally characterized GlyRs are generally engineered to have a very short ICD. We show here that for one such construct, zebrafish GlyR
Gas vesicles (GVs) are large cylindrical gas-filled protein assemblies found in diverse aquatic bacteria that enable their adaptation of buoyancy. GVs have already been used as ultrasound contrasting agents. Here, we investigate derived from Bacillus megaterium, aiming to minimize the number accessory Gvps within GV gene cluster and demonstrate use enhancers acoustic radiation force administered by ultrasound. Three (GvpR, GvpT, GvpU) out 11 genes were be dispensable for functional...
Highly regulated intracellular calcium entry affects numerous cellular physiological events. External regulation of signaling presents a great opportunity for the artificial activity. Calcium can be mediated by STIM proteins interacting with Orai channels; therefore, STIM1–Orai1 pair has become tool artificially modulating entry. We report on an innovative genetically engineered protease-activated activator called PACE. CAD self-dimerization and activation were inhibited coiled-coil forming...
SUMMARY Regulation of the activity proteins enables control complex cellular processes. Allosteric regulation has been introduced individually into few natural proteins. Here, we present a generally applicable diverse called INSRTR (inserted peptide structure regulator), based on inserting short unstructured solvent-accessible loop that retains protein function. Function target can be inactivated by addition forms rigid coiled-coil dimer. This platform construction ON/OFF switches, their...