A5055496763- Nerve injury and regeneration
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Nerve Injury and Rehabilitation
- Wound Healing and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Tendon Structure and Treatment
- Peripheral Nerve Disorders
- Spinal Cord Injury Research
- Cellular Mechanics and Interactions
- Periodontal Regeneration and Treatments
- Cerebral Palsy and Movement Disorders
- Spine and Intervertebral Disc Pathology
- Muscle Physiology and Disorders
- Aortic Disease and Treatment Approaches
- Hemostasis and retained surgical items
- Microfluidic and Bio-sensing Technologies
- Hydrogels: synthesis, properties, applications
- Graphene and Nanomaterials Applications
- Spinal Hematomas and Complications
- Bone Tissue Engineering Materials
- Veterinary Orthopedics and Neurology
- Pain Management and Treatment
- Planarian Biology and Electrostimulation
Universidade do Porto
2015-2025
Hospital da Luz
2024
Centro Hospitalar de Lisboa Ocidental
2024
Polyvinyl alcohol hydrogel (PVA) is a synthetic polymer with an increasing application in the biomedical field that can potentially be used for vascular grafting. However, tissue and blood–material interactions of such gels membranes are unknown detail. The objectives this study were to: (a) assess biocompatibility (b) hemocompatibility PVA-based order to get some insight into its potential use as graft. PVA was evaluated isolated or copolymerization dextran (DX), biopolymer known effects...
Bacterial cellulose was functionalized with a chimeric protein containing cellulose-binding module and the adhesion peptide Arg-Gly-Asp. Small-diameter bacterial membranes were produced subcutaneously implanted in sheep for 1–32 weeks. The implants triggered biological response similar to other high surface-to-volume implants. There no significant differences inflammation degree between coated recombinant Arg-Gly-Asp–cellulose-binding native cellulose. considered be mildly irritating tissue...
Human mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers clinical use. UCB plasma (hUCBP) can be used as supplement hMSCs culture, since rich soluble growth factors due worldwide increased number cryopreserved units public private banks, without the disadvantages listed FBS. On other...
The healing process of the skin is a dynamic procedure mediated through complex feedback growth factors secreted by variety cells types.Despite most recent advances in wound management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem (MSCs) are nowadays promising treatment ulcers which cause high morbidity.The MSCs modulate inflammatory local response induce cell replacing, paracrine mode action, being an important therapy for...
Peripheral nerve injury remains a clinical challenge with severe physiological and functional consequences. Despite the existence of multiple possible therapeutic approaches, until now, there is no consensus regarding advantages each option or best methodology in promoting regeneration. Regenerative medicine promise to overcome this medical limitation, work, chitosan guide conduits olfactory mucosa mesenchymal stem/stromal cells were applied different combinations promote regeneration...
The purpose of this study was to test in vivo two different nerve guides for promoting regeneration across a 10-mm gap the rat sciatic nerve: 1) one made PLGA novel proportion (90:10) polymers poly(L-lactide):poly(glycolide); 2) another (DL-lactide-epsilon-caprolactone) copolyester (Neurolac) tube, by comparing its healing efficacy with that more traditional methods end-to-end suture and autologous graft. Motor sensory functional recovery were assessed throughout period 20 weeks, repaired...
Abstract Peripheral nerves possess the capacity of self-regeneration after traumatic injury but extent regeneration is often poor and may benefit from exogenous factors that enhance growth. The use cellular systems a rational approach for delivering neurotrophic at nerve lesion site, in present study we investigated effects enwrapping site end-to-end rat sciatic repair with an equine type III collagen membrane enriched or not N1E-115 pre-differentiated neural cells. After neurotmesis, was...
Skeletal muscle has good regenerative capacity, but the extent of injury and developed fibrosis might prevent complete regeneration. The in vivo application human mesenchymal stem cells (HMSCs) umbilical cord conditioned media (CM) where HMSCs were cultured expanded, associated with different vehicles to induce regeneration, was evaluated a rat myectomy model. Two commercially available spherical hydrogel by our research group used. treated groups obtained interesting results terms both...
Peripheral nerves have the intrinsic capacity of self-regeneration after traumatic injury but extent regeneration is often very poor. Increasing evidence demonstrates that mesenchymal stem/stromal cells (MSCs) may play an important role in tissue through secretion soluble trophic factors enhance and assist repair by paracrine activation surrounding cells. In present study, therapeutic value a population umbilical cord tissue-derived MSCs, obtained proprietary method (UCX(®)), was evaluated...
In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon nerve-supporting Schwann cells. present study, effect of human from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide- ε -caprolactone) membrane, nerve regeneration, was evaluated in neurotmesis injury rat sciatic model. Results vitro showed successful differentiation...
Peripheral nerve injuries remain a significant clinical challenge, particularly in severe neurotmesis requiring complex therapeutic interventions to restore functionality. This study evaluated the pro-regenerative potential of combining neural guide conduits with secretome from olfactory mucosa mesenchymal stem cells, compared gold-standard surgical techniques, validated ovine model common peroneal injury. Recovery was assessed over 24 weeks using functional, kinematic, ultrasonographic, and...
Poly(lactic-co-glycolic acid) (PLGA) nerve tube guides, made of a novel proportion (90:10) the two polymers, poly(L-lactide): poly(glycolide) and covered with neural cell line differentiated in vitro, were tested vivo for promoting regeneration across 10-mm gap rat sciatic nerve. Before testing, PLGA 90:10 tubes vitro water uptake mass loss compared collagen sheets. The was lower, more rapid higher than those sheets when immersed phosphate-buffered saline (PBS) solution. pH values immersing...
Polyvinyl alcohol hydrogel (PVA) is a water-soluble synthetic polymer that commonly used in biomedical applications including vascular grafting. It was argued the copolymerization of PVA with dextran (Dx) can result improvement blood–biomaterial interactions. The focus this experimental study to assess interaction through an vivo and vitro evaluation coagulation system activation. thrombogenicity copolymer determined by quantification platelet adhesion lactate dehydrogenase assay,...
Introduction Peripheral nerve injuries remain a significant clinical challenge, particularly in severe neurotmesis requiring complex therapeutic interventions to restore functionality. This study aimed evaluate the pro-regenerative potential of combining neural guide conduits with conditioned medium from olfactory mucosa mesenchymal stem cells, compared gold-standard surgical techniques. Methods The was conducted using validated ovine model common peroneal injury. Recovery assessed over 24...
Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal this study was assess effect on regeneration, associating a hybrid chitosan membrane with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly umbilical cord, in peripheral reconstruction after crush injury. Chromosome analysis cell line carried out and no structural alterations were found metaphase. Chitosan membranes previously tested vitro,...
Abstract The goal of the present study was to assess whether in vitro‐differentiated N1E‐115 cells supported by a collagen membrane would enhance rat sciatic nerve regeneration after crush injury. To set up an appropriate experimental model for investigating effects neural cell transplantation, we have recently described sequence functional and morphologic changes occurring standardized injury with nonserrated clamp. Functional recovery evaluated using index, static extensor postural thrust,...