A5043390726- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Magnetic and transport properties of perovskites and related materials
- Plant and Fungal Species Descriptions
- Multiferroics and related materials
- Advanced Condensed Matter Physics
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Induction Heating and Inverter Technology
- CRISPR and Genetic Engineering
- Wildlife Ecology and Conservation
- Genetic diversity and population structure
- GaN-based semiconductor devices and materials
- Rangeland Management and Livestock Ecology
- Physics of Superconductivity and Magnetism
- interferon and immune responses
- Telomeres, Telomerase, and Senescence
- Viral Infections and Immunology Research
- Identification and Quantification in Food
- Veterinary medicine and infectious diseases
National Yang Ming Chiao Tung University
2003-2024
Academia Sinica
2021-2022
Rutgers, The State University of New Jersey
2000
Institute of Zoology
1964
Centre for Healthy Start Initiative
1962
Transcriptional silencing of the HM loci in yeast requires cis -acting elements, termed silencers, that function during S-phase passage to establish silent state. To study role regulatory elements maintenance repression, site-specific recombination was used uncouple preassembled chromatin fragments from silencers. DNA rings excised HMR were initially but ultimately reactivated, even G 1 - or 2 /M-arrested cells. In contrast, bearing HML -derived sequence stably repressed due presence a...
Transcriptional repression of the silent mating-type loci in Saccharomyces cerevisiae requires a cell cycle–dependent establishment step that is commonly assumed to involve DNA replication. Using site-specific recombination, we created nonreplicating ring vivo test directly role replication silencing. Sir1 was tethered following excision from chromosome activate dormant silencer. We show here silencing can be established does not replicate. The silenced adopted structural features...
Production of protein containing lengthy stretches polyglutamine encoded by multiple repeats the trinucleotide CAG is a hallmark Huntington's disease (HD) and variety other inherited degenerative neurological neuromuscular disorders. Earlier work has shown that interference with production transcription elongation SUPT4H results in decreased cellular capacity to transcribe mutant huntingtin gene (Htt) alleles long expansions, but little effect on expression genes short stretches. zQ175 R6/2...
Abstract The long non-coding telomeric RNA transcript TERRA, in the form of an RNA–DNA duplex, regulates telomere recombination. In a screen for nucleases that affects recombination, mutations DNA2, EXO1, MRE11 and SAE2 cause severe delay type II survivor formation, indicating recombination is mediated through mechanism similar to repairing double-strand breaks. On other hand, mutation RAD27 results early formation suggesting acts as negative regulator encodes flap endonuclease plays role...
Abstract Background Polyglutamine (polyQ) diseases are dominant neurodegenerative caused by an expansion of the polyQ‐encoding CAG repeats in disease‐causing gene. The length is major determiner age at onset (AO) polyQ diseases, including Huntington's disease (HD) and spinocerebellar ataxia type 3 (SCA3). Objective We set out to identify common genetic variant(s) that may affect AO diseases. Methods Three hundred thirty‐seven patients with HD or SCA3 were enrolled for targeted sequencing 583...
Earlier work has shown that siRNA-mediated reduction of the SUPT4H or SUPT5H proteins, which interact to form DSIF complex and facilitate transcript elongation by RNA polymerase II (RNAPII), can decrease expression mutant gene alleles containing nucleotide repeat expansions differentially. Using luminescence fluorescence assays, we identified chemical compounds interfere with SUPT4H-SUPT5H interaction then investigated their effects on synthesis mRNA protein encoded in huntingtin ( HTT ),...
Single-phase ${\mathrm{La}}_{0.7}{\mathrm{Sn}}_{0.3}\mathrm{Mn}{\mathrm{O}}_{3}$ (LSnMO) thin films were fabricated on $\mathrm{La}\mathrm{Al}{\mathrm{O}}_{3}$ (LAO) substrates by pulsed laser deposition (PLD). The as-deposited films, though showed insulating characteristics with no sign of insulator-metal transition (IMT) down to very low temperatures, did display a paramagnetic-ferromagnetic (PFT) around $180\phantom{\rule{0.3em}{0ex}}\mathrm{K}$. x-ray absorption spectroscopy (XAS) the...
Dysregulated protein homeostasis, characterized by abnormal accumulation and aggregation, is a key contributor to the progression of neurodegenerative disorders such as Huntington's disease spinocerebellar ataxia type 3 (SCA3). Previous studies have identified PIAS1 gene variants in patients with late-onset SCA3 disease. This study aims elucidate role its S510G variant modulating pathogenic mechanisms SCA3. Through vitro biochemical analyses vivo assays, we demonstrate that stabilizes both...
The DSIF complex comprising the Supt4h and Supt5h transcription elongation proteins clamps RNA polymerase II (RNAPII) onto DNA templates, facilitating processivity. Lowering components can differentially decrease expression of alleles containing nucleotide repeat expansions, suggesting that RNAPII transit through expansions is dependent on functions. To globally identify sequence features affect dependence in human cells, we used ultra-deep ChIP-seq analysis RNA-seq to investigate quantify...