A5115600286- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Adrenal and Paraganglionic Tumors
- Cancer Genomics and Diagnostics
- Renal cell carcinoma treatment
- Glioma Diagnosis and Treatment
- Cancer therapeutics and mechanisms
- Sarcoma Diagnosis and Treatment
- Lung Cancer Research Studies
- Neuroendocrine Tumor Research Advances
- Virus-based gene therapy research
- Pancreatic and Hepatic Oncology Research
- Neurofibromatosis and Schwannoma Cases
- Medical Imaging Techniques and Applications
- Childhood Cancer Survivors' Quality of Life
- Ion channel regulation and function
- Neonatal Respiratory Health Research
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Genetic factors in colorectal cancer
- Pediatric Urology and Nephrology Studies
- Cell death mechanisms and regulation
- Neonatal Health and Biochemistry
- Epigenetics and DNA Methylation
Princess Máxima Center
2016-2025
Emma Kinderziekenhuis
2011-2025
Utrecht University
2024-2025
Amsterdam University Medical Centers
2019-2024
Sanquin
2008-2024
University of Amsterdam
2009-2024
University Medical Center Utrecht
2022-2024
Stichting Kinderoncologie Nederland
2014-2019
Amsterdam UMC Location University of Amsterdam
2002-2017
University Hospital of Wales
2016
Abstract Kidney tumours are among the most common solid in children, comprising distinct subtypes differing many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing disease heterogeneity currently lacking. Here, we describe first paediatric cancer organoid biobank. It contains tumour matching normal kidney organoids from over 50 children with different of cancer, Wilms tumours, malignant rhabdoid renal carcinomas, congenital mesoblastic nephromas....
Pediatric patients with high-risk neuroblastoma have poor survival rates and urgently need more effective treatment options less side effects. Since novel improved immunotherapies may fill this need, we dissect the immunoregulatory interactions in by single-cell RNA-sequencing of 24 tumors (10 pre- 14 post-chemotherapy, including 5 pairs) to identify strategies for optimizing immunotherapy efficacy. Neuroblastomas are infiltrated natural killer (NK), T B cells, immunosuppressive myeloid...
Wilms tumor (WT) is the most common pediatric renal tumor. Treatment planning under International Society of Paediatric Oncology (SIOP) protocols based on staging and histologic assessment response to preoperative chemotherapy. Despite high overall survival (OS), many relapses occur in patients without specific risk factors, successfully treated are exposed treatments with significant risks late effects. To investigate whether molecular biomarkers could improve stratification, we assessed 1q...
PurposeLong-term trends in neuroblastoma incidence and survival unscreened populations are unknown. We explored incidence, stage at diagnosis, treatment of the Netherlands from 1990 to 2014.MethodsThe Cancer Registry provided data on all patients aged <18 years diagnosed with a neuroblastoma. Trends were evaluated by calculating average annual percentage change (AAPC). Univariate multivariable analyses performed for 4 disease test whether changes associated survival.ResultsOf 593 newly...
Polymerase chain reaction (PCR)-based detection of minimal residual disease (MRD) in neuroblastoma can be used to monitor therapy response and evaluate stem cell harvests. Commonly PCR markers, tyrosine hydroxylase (TH) GD2 synthase, have expression normal tissues, thus limiting MRD detection. To identify a more specific marker, we tested PHOX2B.To determine PHOX2B, TH, synthase it was measured by real-time quantitative samples bone marrow (BM; n = 51), peripheral blood (PB; 37),...
PCR-based detection of minimal residual disease (MRD) in neuroblastoma (NB) patients can be used for initial staging and monitoring therapy response bone marrow (BM) peripheral blood (PB). PHOX2B has been identified as a sensitive specific MRD marker; however, its expression varies between tumors. Therefore, panel markers could increase sensitivity.To identify additional NB, we selected genes by comparing SAGE (serial analysis gene expression) libraries healthy NB tissues followed extensive...
Thyroid dysfunction has been reported in up to 52% of patients 1.4 years after treatment with (131) I-Metaiodobenzylguanidine (MIBG) children neuroblastoma (NBL), despite the use potassium-iodide (KI). Our aim was investigate if incidence and severity thyroid damage increases time.All long-term survivors childhood NBL treated I-MIBG period 1989-1999 our center (n = 16 43) were evaluated. During exposure I-MIBG, received 100 mg KI per day as protection. All MIBG images evaluated for uptake...
In the clinical management of paediatric solid tumours, histological examination tumour tissue obtained by a biopsy remains gold standard to establish conclusive pathological diagnosis. The DNA methylation pattern is known correlate with histopathological diagnosis across cancer types and showing promise in diagnostic workup samples. This can be detected cell-free DNA. Here, we provide proof-of-concept histopathologic classification tumours using reduced representation bisulphite sequencing...
Malignant rhabdoid tumors (MRTs) represent one of the most aggressive childhood malignancies. No effective treatment options are available, and prognosis is, therefore, dismal. Previous studies have demonstrated that tumor organoids capture heterogeneity patient can be used to predict response therapy. Here, we perform drug screening on patient-derived normal identify MRT-specific therapeutic vulnerabilities. We neddylation inhibitor MLN4924 as a potential agent. Mechanistically, find...
Abstract In children, renal cell carcinoma (RCC) is rare. This study the first report of pediatric patients with RCC registered by International Society Pediatric Oncology‐Renal Tumor Study Group (SIOP‐RTSG). histologically confirmed RCC, in SIOP 93‐01, 2001 and UK‐IMPORT databases, were included. Event‐free survival (EFS) overall (OS) analyzed using Kaplan‐Meier method. Between 1993 2019, 122 registered. Available detailed data (n = 111) revealed 56 localized, 30 regionally advanced, 25...
Abstract Purpose Meta -[ 18 F]fluorobenzylguanidine ([ F]mFBG) is a positron emission tomography (PET) radiotracer that allows for fast and high-resolution imaging of tumours expressing the norepinephrine transporter. This pilot study investigates feasibility [ F]mFBG PET-CT in neuroblastoma. Methods In prospective, single-centre study, we recruited children with neuroblastoma, referred meta 123 I]iodobenzylguanidine I]mIBG) scanning, consisting total body planar scintigraphy combination...
Total cell-free DNA (cfDNA) and tumor-derived cfDNA (ctDNA) can be used to study genetic aberrations. We analyzed the diagnostic prognostic potential of ctDNA, obtained from pediatric patients with rhabdomyosarcoma.cfDNA was isolated plasma samples 57 enrolled in EpSSG RMS2005 study. To potential, shallow whole genome sequencing (shWGS) reduced representation bisulphite (cfRRBS) were performed a subset all tested using droplet digital polymerase chain reaction detect methylated RASSF1A...