A5058219913- Gene Regulatory Network Analysis
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Virus-based gene therapy research
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Renal and related cancers
- Sarcoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Electron and X-Ray Spectroscopy Techniques
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
University of California, Davis
2018-2021
The Ohio State University
2020-2021
Oregon Health & Science University
2019-2020
Intratumoral heterogeneity is associated with aggressive tumor behavior, therapy resistance, and poor patient outcomes. Such thought to be dynamic, shifting over periods of minutes hours in response signaling inputs from the microenvironment. However, models this process have been inferred indirect or post-hoc measurements cell state, leaving temporal details signaling-driven undefined. Here, we developed a live-cell model system which microenvironment-driven dynamics can directly observed...
Microscopy allows researchers to interrogate proteins within a cellular context. To deliver protein-specific contrast, we developed new class of genetically encoded peptide tags called versatile interacting (VIP) tags. VIP reporter target protein via the formation heterodimer between tag and an exogenously added probe peptide. We report herein named MiniVIPER, which is comprised MiniE–MiniR heterodimer. first demonstrated selectivity MiniVIPER by labeling three targets: transferrin receptor...
Verdinexor (KPT-335) is a novel orally bioavailable selective inhibitor of nuclear export (SINE) compound that inhibits the function protein Exportin 1 (XPO1/CRM1). In present study, we sought to characterize expression XPO1 in primary canine osteosarcoma (OS) tumour samples, OS cell lines and normal osteoblasts evaluate vitro activity verdinexor alone or combination with doxorubicin. Canine subset tumours showed increased transcript as compared osteoblast cells. All exhibited dose-dependent...
Histone demethylases are overexpressed or display altered activity in numerous human cancers leading to alterations cell cycle dynamics, DNA repair kinetics, and therapeutic resistance. Consequently, targeting of histone has become an active promising area research oncology. However, the role potential efficacy demethylase inhibition canine remains largely unknown. In present work, we addressed this knowledge gap by exploring inhibitors (HDIs) oral melanoma. Using melanoma lines, determined...
Versatile Interacting Peptide (VIP) tags are a new class of genetically-encoded tag designed for imaging cellular proteins by fluorescence and electron microscopy.In 2018, we reported the VIPER (Doh et al., 2018), which contains two elements: peptide (i.e., CoilE) probe CoilR).These peptides deliver contrast to protein interest forming specific, high-affinity heterodimer.The was with single cysteine residue site-specific modification via thiol-maleimide chemistry.This feature can be used...
Abstract The EGFR/Ras/ERK signalling pathway is a driver of cancer cell proliferation and metastasis in tumours that exhibit high cell-to-cell heterogeneity. While the activity this frequently amplified tumours, it not understood how kinetic aspects its activation differ from normal cellular signalling. Using live-cell reporters ERK breast progression series HMT-3522, we found invasive cells similar amplitude to isogenic non-malignant but highly dynamic more disordered, leading heterogeneous...