A5078317655- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Salmonella and Campylobacter epidemiology
- Immune Response and Inflammation
- Fibroblast Growth Factor Research
- Vibrio bacteria research studies
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune responses and vaccinations
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ferroptosis and cancer prognosis
- Fatty Acid Research and Health
- Phagocytosis and Immune Regulation
- Video Surveillance and Tracking Methods
Université de Montpellier
2018-2024
Centre National de la Recherche Scientifique
2018-2024
Inserm
2023-2024
Institute for Research in Biomedicine
2022
Biologie et Génétique des Interactions Plante-Parasite
2020-2022
Laboratory of Pathogens and Host Immunity
2018-2020
Understanding the roles of neutrophils and macrophages in fighting bacterial infections is a critical issue human pathologies. Although phagocytic killing has been extensively studied, little known about how bacteria are eliminated extracellularly live vertebrates. We have recently developed an infection model zebrafish embryo which leukocytes cannot reach injected bacteria. When Escherichia coli within notochord, both massively recruited during several days, but do not infiltrate infected...
Numerous intracellular bacterial pathogens interfere with macrophage function, including polarization, to establish a niche and persist. However, the spatiotemporal dynamics of polarization during infection within host remain be investigated. Here, we implement model persistent Salmonella Typhimurium in zebrafish, which allows visualization polarized macrophages bacteria real time at high resolution. While polarize toward M1-like phenotype control early infection, later stages, persists...
Immediately after a wound, macrophages are activated and change their phenotypes in reaction to danger signals released from the damaged tissues. The cues that contribute macrophage activation wounding vivo still poorly understood. Calcium signaling Reactive Oxygen Species (ROS), mainly hydrogen peroxide, conserved early wound emanate guide neutrophils within tissues up wound. However, role of these recruitment is elusive. Here we used transparent zebrafish larva as tractable vertebrate...
In response to wound signals, macrophages are immediately recruited the injury where they acquire distinct phenotypes and functions, playing crucial roles both in host defense healing process. Although macrophage have been intensively studied during healing, mostly using markers expression profiles, impact of environment on shape behaviour, underlying mechanisms deserve more in-depth investigation. Here, we sought characterize dynamics recruitment behaviour aseptic wounding caudal fin fold...
Abstract While considered an extracellular pathogen, Pseudomonas aeruginosa has been reported to be engulfed by macrophages in cellular and animal models. However, the role of P. clearance vivo remains poorly studied. The major outer membrane porin OprF recently shown involved fate within cultured analysis oprF mutant may thus provide insights better understand relevance this intramacrophage stage during infection. In present study, we investigated for first time virulence a vertebrate model...
Background and Purpose Specialized pro‐resolving mediators (SPMs) are a family of lipids controlling the resolution inflammation playing role in many processes including organ protection tissue repair. While SPMs potent bioactive molecules vivo , their epimorphic regeneration organs vertebrates has not been tested. Using zebrafish larva as robust regenerative vertebrate system, we studied SPM neuroprotectin/protectin D1 (PD1) during caudal fin fold regeneration. Experimental Approach...
ABSTRACT Innate immune responses to inflammation and infection are complex represent major challenges for developing much needed new treatments chronic inflammatory diseases drug-resistant infections. To be ultimately successful, the response must balanced allow pathogen clearance without excess tissue damage, processes controlled by pro- anti-inflammatory signals. The roles of signalling in raising an appropriate underappreciated, representing overlooked potential drug targets. This is...
ABSTRACT Numerous intracellular bacterial pathogens interfere with macrophage function, including polarization, to establish a niche and persist. However, the spatiotemporal dynamics of polarization during infection within host remain be investigated. Here, we implement model persistent Salmonella Typhimurium in zebrafish, which allows visualization polarized macrophages bacteria real time at high-resolution. While polarize toward M1-like phenotype control early infection, later stages,...
Abstract We introduce two level-set method approaches to segmentation of 2D macrophage images. The first one is based on the Otsu thresholding and second information entropy thresholding, both followed by classical subjective surface (SUBSURF) method. Results methods are compared with semi-automatic Lagrangian in which curve evolves along edge it controlled an expert user. present comparison all three respect Hausdorff distance resulting curves we compare also their perimeter enclosed area....