A5101527386- Effects of Radiation Exposure
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Alzheimer's disease research and treatments
- Radiation Therapy and Dosimetry
- Immunotherapy and Immune Responses
- Peripheral Neuropathies and Disorders
- PARP inhibition in cancer therapy
- Multiple Sclerosis Research Studies
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Radiation Effects and Dosimetry
- Hematopoietic Stem Cell Transplantation
- Dermatology and Skin Diseases
- Prion Diseases and Protein Misfolding
- Cancer, Hypoxia, and Metabolism
- T-cell and Retrovirus Studies
- Histone Deacetylase Inhibitors Research
- Immune cells in cancer
- CAR-T cell therapy research
- Endometriosis Research and Treatment
- Inflammatory Myopathies and Dermatomyositis
- Chronic Myeloid Leukemia Treatments
- Cholinesterase and Neurodegenerative Diseases
- Intracerebral and Subarachnoid Hemorrhage Research
Ehime Prefectural University of Health Science
2018-2024
Meiji University
2020
Ehime University
2013-2020
Saiseikai Fukuoka General Hospital
2013-2018
Baylor College of Medicine
2006-2015
The University of Texas Health Science Center at Houston
2015
Molina Center for Energy and the Environment
2015
Texas Children's Hospital
2012-2015
Koga Hospital
2014
Children's Nutrition Research Center at Baylor College of Medicine
2011
The amyloid beta-protein (Abeta) ending at 42 plays a pivotal role in Alzheimer's disease (AD). We have reported previously that intracellular Abeta42 is associated with neuronal apoptosis vitro and vivo. Here, we show directly activated the p53 promoter, resulting p53-dependent apoptosis, Abeta40 had similar but lesser effect. Moreover, oxidative DNA damage induced nuclear localization of mRNA elevation guinea-pig primary neurons. Also, expression was elevated brain sporadic AD transgenic...
The antitumor activity of activated CD 8+ T cells in the tumor microenvironment seems to be limited due their being metabolically unfit. This metabolic unfitness is closely associated with T‐cell exhaustion and impairment memory formation, which are barriers successful adoptive immunotherapy. We therefore assessed role glutamine metabolism 8 + using a tumor‐inoculated mouse model. transfer tumor‐specific cultured under glutamine‐restricted ( dG ln) conditions or treated specific inhibitors...
Abstract Using the polymerase chain reaction, we quantitated amount of human T‐lymphotropic virus type I (HTLV‐I) proviral DNA in peripheral blood mononuclear cells from 18 patients with HTLV‐I–associated myelopathy/tropical spastic paraparesis; 17 HTLV‐I carriers without paraparesis, or other autoimmune inflammatory diseases; and 19 seronegative control subjects. The was 10‐ to 100‐fold higher HAM/TSP who had diseases than diseases. onset myelopathy at a younger age (15 39 years) an...
The induction of thymic lymphomas by whole-body X irradiation with four doses 1.8 Gy (total dose: 7.2 Gy) in C57BL/6 mice was suppressed from a high frequency (90%) to 63% preirradiation 0.075 rays given 6 h before each 1.8-Gy irradiation. This level further 43% continuous 137Cs gamma at low dose rate 1.2 mGy/h for 450 days, starting 35 days the challenging Continuous resulting total over 258 yielded no lymphomas, indicating that this low-dose-rate radiation does not induce these tumors....
Although CD4 T-cell senescence plays an important role in immunosenescence, the mechanism behind this process remains unclear. Here we show that T cell-specific Menin deficiency results premature of cells, which is accompanied by senescence-associated secretory phenotype after antigenic stimulation and dysregulated cytokine production. required for expansion survival antigen-stimulated cells vivo acts targeting Bach2, known to regulate immune homeostasis binds Bach2 locus controls its...
While menin plays an important role in preventing T-cell dysfunction, such as senescence and exhaustion, the regulatory mechanisms remain unclear. We found that prevents induction of dysfunction activated CD8 T cells by restricting cellular metabolism. mTOR complex 1 (mTORC1) signaling, glycolysis, glutaminolysis are augmented deficiency. Rapamycin treatment menin-deficient cells. Limited glutamine availability also induced deficiency, its inhibitory effect is antagonized α-ketoglutarate...
Bone marrow hematopoietic stem cells (HSCs) balance proliferation and differentiation by integrating complex transcriptional post-translational mechanisms regulated cell intrinsic extrinsic factors. We found that transcripts of G(0)/G(1) switch gene 2 (G0S2) are enriched in lineage(-) Sca-1(+) c-kit(+) (LSK) CD150(+) CD48(-) CD41(-) cells, a population highly for quiescent HSCs, whereas G0S2 expression is suppressed dividing LSK cells. Gain-of-function analyses using retroviral vectors bone...
Abstract Although the important roles of glycolysis in T cells have been demonstrated, regulatory mechanism activated has not fully elucidated. Furthermore, influences glycolytic failure on cell-dependent immune response vivo remain unclear. We therefore assessed role using cell-specific Pgam1 -deficient mice. Both CD8 and CD4 responses were attenuated by deficiency. The helper inflammation was ameliorated Glycolysis augments activation mTOR complex 1 (mTORC1) T-cell receptor (TCR) signals....
Amyloid β protein ending at 42 (Aβ42) plays an important role in the pathology of Alzheimer's disease (AD). Here we show increase cellular Aβ42 damaged neurons, with both ELISA and immunocytochemistry. The was caused by 3-day treatments H2O2, etoposide or melphalan, all which induce genotoxic apoptosis, but not treatment sodium azide, causes necrosis. Secreted Aβ similarly decreased these treatments. appeared even minimal damage (ELISA) Aβ42-positive cells were TUNEL negative (double...
Objective To clarify the clinical features of MS patients with hyperprolactinemia. Subjects and Methods The serum prolactin level was measured in 67 Japanese (19 men 48 women) multiple sclerosis (MS) 16 (4 12 HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) using a two-site immunoradiometric assay. Results In patients, 32 were classified as having Asian type showing selective involvement optic nerves spinal cord, while other 35 Western which displayed disseminated central...
BACKGROUND: Insulin‐like growth factor‐I (IGF‐I) plays crucial roles in uterine leiomyoma cell through stimulating proliferation and inhibiting apoptosis. The present study was conducted to elucidate whether progesterone affects IGF‐I its receptor expression cultured cells. METHODS: Isolated cells were subcultured Phenol Red‐free Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum for 120 h then stepped down serum‐free conditions an additional 48 the presence or...
Dietary influences may affect microbiome composition and host immune responses, thereby modulating propensity toward inflammatory bowel diseases (IBDs): Crohn disease (CD) ulcerative colitis (UC). n-6 fatty acids have been associated with UC in prospective studies. However, the critical developmental period when (n-6) consumption induce is not known. We examined effects of transiently increased during pediatric development on subsequent dextran-sulfate-sodium (DSS)-induced acute murine...
A photoluminescence glass dosimeter, GD-301, was applied to the measurement of low absorbed doses in mice exposed low-dose rate 137Cs gamma-rays. The dosimeter system consists small rod-shaped chip detectors capable embedded body a mouse and an automatic readout device equipped with standard detector irradiated gamma-source. measured were compared "exposure" estimated by ionization chamber BeO:Na thermoluminescence system. results clearly demonstrate superiority dosimetry regarding...
Abstract T cell receptor activation inhibits expression of the E74-like factor (ELF) 4 and Krüppel-like genes to release naive CD8+ cells from their quiescent state. In this study, we show that ELF4 controls ERK-mediated proliferative response by maintaining normal levels dual-specificity phosphatases 1 5 in cells. activated cells, mammalian target rapamycin pathway downstream ERK PI3K signaling. Our findings demonstrate could be used modulate transcriptional network involved cell-cycle regulation.
Abstract Although the methylation status of histone H3K27 plays a critical role in CD4+ T cell differentiation and its function, Utx demethylase CD8+ cell–dependent immune response remains unclear. We therefore generated cell–specific Utxflox/flox Cd4-Cre Tg (Utx KO) mice to determine cells. Wild-type (WT) KO were infected with Listeria monocytogenes expressing OVA analyze Ag-specific There was no significant difference number cells upon primary infection between WT mice. However, deficiency...
Since its discovery, diverse functions have been attributed to the G 0 /G 1 switch gene 2 (G0S2), from lipid metabolism control of cell proliferation. Our group showed for first time that G0S2 promotes quiescence in hematopoietic stem cells by interacting with and retaining nucleolin around nucleus. Herein, we report role differentiation function CD8 + T examined mice an embryonic deletion G0s2 gene. expression naïve decreased immediately after T‐cell receptor activation downstream...