A5007520892- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- Protein purification and stability
- Virus-based gene therapy research
- Nanoparticle-Based Drug Delivery
- Cancer, Hypoxia, and Metabolism
- Graphene and Nanomaterials Applications
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- S100 Proteins and Annexins
- Phagocytosis and Immune Regulation
- Biomedical and Engineering Education
- Bacteriophages and microbial interactions
- Space exploration and regulation
- Microbial Metabolic Engineering and Bioproduction
- Biosimilars and Bioanalytical Methods
- Biochemical and Molecular Research
- Carbon Nanotubes in Composites
- Monoclonal and Polyclonal Antibodies Research
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Photodynamic Therapy Research Studies
- Amino Acid Enzymes and Metabolism
- Vitamin C and Antioxidants Research
- Cancer-related gene regulation
University of Oklahoma
2016-2025
OU Health Stephenson Cancer Center
2020-2024
Oklahoma State University Oklahoma City
2020-2024
Naval Postgraduate School
2023
University of Oklahoma Health Sciences Center
2005-2016
Translational Research in Oncology
1991-2015
Missouri University of Science and Technology
2015
Eisenhower Medical Center
2014
United States Air Force Academy
2014
University of Illinois Urbana-Champaign
2005
Three native E. coli proteins—NusA, GrpE, and bacterioferritin (BFR)—were studied in fusion proteins expressed for their ability to confer solubility on a target insoluble protein at the C-terminus of protein. These three were chosen based favorable cytoplasmic characteristics as predicted by statistical model recombinant coli. Modeling probability soluble expression human interleukin-3 (hIL-3) following order: NusA (most soluble), BFR, thioredoxin (least soluble). Expression experiments...
Abstract The greatest contributors to cancer mortality are metastasis and the consequences of its treatment. Here, we present a novel treatment metastatic breast that combines photothermal therapy with targeted single-walled carbon nanotubes (SWCNTs) immunostimulation checkpoint inhibitor. We find selective near-infrared ablation primary orthotopic EMT6 tumors in syngeneic BALB/cJ mice using an annexin A5 (ANXA5) functionalized SWCNT bioconjugate synergistically enhances anti-cytotoxic...
In this study, we describe the use of a sodium cholate suspension-dialysis method to adsorb redox enzyme glucose oxidase (GOX) onto single-walled carbon nanotubes (SWNT). By method, solutions dispersed and debundled SWNTs were prepared that remained stable for 30 days which retained 75% native enzymatic activity. We also demonstrate GOX-SWNT conjugates can be assembled into amperometric biosensors with poly[(vinylpyridine)Os(bipyridyl)2Cl2+/3+] polymer (PVP-Os) through layer-by-layer (LBL)...
Expression of human tumor necrosis factor-alpha (TNF) and four different TNF analogs has been studied in Pichia pastoris by utilizing the alcohol oxidase gene promoter. expression level certain transformants accounted for as much 36% soluble protein. was stably maintained during high cell density fermentation (100 g dry weight/liter) resulting a production 6-10 g/liter. contained P. lysates biologically active determined its cytotoxic effect on murine L-929 fibroblast cells bioactivity...
Abstract In this article we present a new and more accurate model for the prediction of solubility proteins overexpressed in bacterium Escherichia coli . The uses statistical technique logistic regression. To build model, 32 parameters that could potentially correlate well with were used. addition, protein database was expanded compared to those used previously. We tested several different implementations regression varied results. best implementation, which is one report, exhibits excellent...
This paper focuses on the targeting of single-walled carbon nanotubes (SWNTs) for treatment breast cancer with minimal side effects using photothermal therapy. The human protein annexin V (AV) binds specifically to anionic phospholipids expressed externally surface tumour cells and endothelial that line vasculature. A 2 h incubation SWNT-AV conjugate proliferating followed by washing near-infrared (NIR) irradiation at a wavelength 980 nm was enough induce significant cell death; there no...
Bladder cancer has a 60%-70% recurrence rate most likely due to any residual tumour left behind after transurethral resection (TUR). Failure completely resect the can lead and progression into higher grade tumours with metastatic potential. We present here novel therapy treat superficial potential decrease recurrence. The is heat-based approach in which bladder specific single-walled carbon nanotubes (SWCNTs) are delivered intravesically at very low dose (0.1 mg SWCNT per kg body weight)...
Abstract Multiple linear regression was used to quantify the dependence of antimicrobial activity 13 peptides upon three calculated or experimentally determined parameters: mean hydrophobicity, hydrophobic moment, and α‐helix content. Mean moment is a measure amphiphilicity in an α‐helical conformation. Antimicrobial quantified as reciprocal measured minimal inhibitory concentration ( MIC ) against Escherichia coli . One magainin 2, remainder were novel designed for this study. The multiple...
We used heparosan (HEP) polysaccharides for controlling nanoparticle delivery to innate immune cells. Our results show that HEP-coated nanoparticles were endocytosed in a time-dependent manner by cells via both clathrin-mediated and macropinocytosis pathways. Upon endocytosis, we observed intracellular vesicles the cytoplasm, demonstrating potential escape from vesicles. Competition with other glycosaminoglycan types inhibited endocytosis of only partially. further found uptake into can be...
A fusion protein was genetically engineered that contains an antimicrobial peptide, designated P2, at its carboxy terminus and bovine prochymosin amino terminus. Bovine chosen as the partner because of complete insolubility in Escherichia coli, a property utilized to protect cells from toxic effects peptide. This purified by centrifugation insoluble inclusion body. methionine linker between P2 peptide enabled be released digestion with cyanogen bromide. Cation exchange HPLC followed...
Click to increase image sizeClick decrease size Acknowledgments The opinions, conclusions, and recommendations expressed or implied in this article are those of the author do not necessarily reflect Eisenhower Center for Space Defense Studies, Air Force Academy, Force, Department Defense, nor any other agency United States Government. Roger G. Harrison is former Director Studies at U.S. as well current Editor Publisher center's journal Defense.
The objective of this study is to demonstrate the adsorption horseradish peroxidase (HRP) on single-walled carbon nanotubes (SWNTs) using sodium cholate suspension–dialysis method and determine effect HRP biological activity UV–vis–NIR spectra SNWTs. results indicate that in a stable SWNT–protein suspension with complete retention enzymatic adsorbed also substantial fraction NIR absorption at 980 nm. loading protein SWNTs high, overall yield preparing high. This process promising for...
The high prevalence of breast cancer is a global health concern, but there are no safe or effective treatments for it at its advanced stages. These facts urge the development novel treatment strategies. Annexin A5 (ANXA5) natural human protein that binds with specificity to phosphatidylserine, phospholipid tightly maintained in inner leaflet cell membrane on most healthy cells externalized tumor and vasculature. Here, we have developed targeted photosensitizer photothermal therapy (PTT)...
A new approach for targeting carbon nanotubes to the tumor vasculature was tested using human endothelial cells and MCF-7 breast cancer in vitro. Single-walled were functionalized with F3 peptide a polyethylene glycol linker target nucleolin, protein found on surface of solid tumors. Confocal microscopy Raman analysis confirmed that conjugate internalized by actively dividing cells. Dividing used mimic these vasculature. Incubation 8 h or more caused significant cell death both cells, an...
The L-methioninase-annexin V/selenomethionine enzyme prodrug system, designed to target the tumor vasculature and release methylselenol anticancer drug in tumor, was tested mice with implanted MBA-MB-231 breast tumors. This therapy able cause a reduction size of tumors during treatment period. It shown that V uniformly bound at blood vessel surface also there substantial cutoff flowing through treated consistent therapy's design. new approach for cancer appears promising.