A5076614105- Antibiotics Pharmacokinetics and Efficacy
- Tuberculosis Research and Epidemiology
- HIV/AIDS drug development and treatment
- HIV-related health complications and treatments
- Drug Transport and Resistance Mechanisms
- Malaria Research and Control
- HIV Research and Treatment
- Pharmaceutical Economics and Policy
- Pneumonia and Respiratory Infections
- Genetics, Bioinformatics, and Biomedical Research
- Antimicrobial Resistance in Staphylococcus
- HIV/AIDS Research and Interventions
- Biochemical and Molecular Research
- Pharmacogenetics and Drug Metabolism
- Cholinesterase and Neurodegenerative Diseases
- Biotechnology and Related Fields
- Bacterial Identification and Susceptibility Testing
- Neonatal Health and Biochemistry
- Bone and Joint Diseases
- Neonatal and Maternal Infections
- Medication Adherence and Compliance
- Carbohydrate Chemistry and Synthesis
- HIV/AIDS Impact and Responses
- Pharmacovigilance and Adverse Drug Reactions
- Genetics and Physical Performance
University of Cape Town
2021-2024
South African Medical Research Council
2024
University of the Western Cape
2017-2021
North-West University
2016-2017
Pharmac
2016-2017
University of Zambia
2015
The pharmacokinetics of vancomycin, a drug used for the treatment methicillin-resistant Staphylococcus aureus (MRSA), varies between paediatric and adult patients. objective this study was to assess vancomycin in preterm neonates determine optimum dose regimen. This randomised double-blind admitted neonatal intensive care units. They all received 15 mg/kg every 12 h. Blood sampled just before administration third, sixth ninth dose. Pharmacokinetic parameters were estimated using Bayesian...
We explore model-informed approaches to address pharmacokinetics variability in African populations due genetic differences the expression and activity of drug metabolizing enzymes potential impact Mycobacterium tuberculosis-mediated metabolism. Application physiologically based pharmacokinetic models that factor variants predicted from artificial intelligence or built using vitro metabolism data liver subcellular fractions with integrated effects tuberculosis can help design doses tailored...
Determination of plasma tenofovir (TFV) concentrations in small volumes and a short time period is most desirable clinical setting. The HPLC-MS/MS method was developed validated for the determination TFV. Plasma sample 10 μL were extracted by protein precipitation. Cimetidine, used as internal standard (ISTD) TFV separated on C18 (Phenomenex Kinetex TM 30 mm × 2.1 mm, 2.6 μm) reversed phase column with pre-column. gradient mobile consisted mmol/L ammonium acetate water acetonitrile/methanol...
The dosing of cycloserine and terizidone is the same, as both drugs are considered equivalent or used interchangeably. Nevertheless, it not certain from literature that these interchangeable. Therefore, amount resulting metabolism relationship with hepatic function were determined.This prospective clinical study involved 39 patients drug-resistant tuberculosis admitted for an intensive phase treatment. Cycloserine pharmacokinetic parameters individual patients, like area under curve (AUC),...
The increase in physical activity (PA) levels has been known to be associated with improved cardiorespiratory fitness status which helps reducing the risk factors of non-communicable disease. However, interaction between and anthropometric variables remains unclear needs further investigation. This study assessed relationship among school-going adolescents Nigeria. Two hundred fifty apparently healthy participants ranging from 12 20 years age, who were randomly selected, participated this...
Aims Despite terizidone being part of the second‐line recommended drugs for treatment drug‐resistant tuberculosis (DR‐TB), information on its pharmacokinetics is scarce. The aim this study was to describe steady‐state population (PPK) and primary metabolite cycloserine in patients with DR‐TB determine effect patient characteristics. Methods This clinical involved 39 adult admitted Brewelskloof Hospital Cape Town, South Africa intensive phase. Blood samples were collected at predose 0.5, 1,...
Abstract Background Chronic patients are required to access their chronic medicines on a regular basis, often only refill repeat prescriptions. Adherence is challenging and has stimulated health care providers devise differentiated service delivery models of decentralise medicine distribution decrease the frequency collection at facilities. One such option includes last kilometre service. This study investigated patients’ preferences for model. Methods An exploratory non-randomised...
Background: Tenofovir disoproxil fumarate (TDF) has been associated with kidney tubulardys function and reduced renal function. Limited studies were performed in Europe Asia that related plasma tenofovir (TFV) concentration function; no such to date have on Africans.Objective: To investigate the correlation between certain markers HIV-infected women TDF antiretroviral therapy (ART).These also compared a HIV-uninfected control group.Methods: (n = 30) TDF-based ART matched 30 controls forage...
Abstract A chromatographic method has been developed and validated for the first time analysis of terizidone in plasma. Terizidone was extracted from plasma by protein precipitation using a mixture acetonitrile methanol (1:1, v/v). The separation achieved with gradient water both containing 0.1% formic acid on Supelco Discovery ® HS C 18 (150 × 4.6 mm, 5 μm) reversed‐phase column. Propranolol used as internal standard. total run‐time min. calibration standard concentrations ranged between...
The translation of a preclinical antimalarial drug development candidate to the clinical phases should be supported by rational human dose selection. A model-informed strategy based on data, which incorporates pharmacokinetic-pharmacodynamic (PK-PD) properties with physiologically pharmacokinetic (PBPK) modeling, is proposed optimally predict an efficacious and dosage regimen for treatment Plasmodium falciparum malaria. viability this approach was explored using chloroquine, has extensive...
Ethionamide is part of the drug-resistant tuberculosis regimen whose pharmacokinetic (PK) and pharmacodynamic (PD) information limited. The aim study was to describe PK simulate doses assess PD attainment.This an observational population patients admitted for at a hospital in South Africa. Nonlinear mixed-effects modelling implemented Monolix 2019R2 used estimate parameters. We performed Monte Carlo simulations optimise dose regimen. target Cmax range 2.5-5 μg/mL, which within minimum...
Tenofovir disoproxil fumarate (TDF) antiretroviral therapy is associated with disruption of the bone turnover process.
Toward addressing the cardiotoxicity liability associated with antimalarial drug astemizole (AST, hERG IC50 = 0.0042 μM) and its derivatives, we designed synthesized analogues based on compound 1 (Pf NF54 0.012 μM; 0.63 μM), our previously identified 3-trifluoromethyl-1,2,4-oxadiazole AST analogue. Compound 11 retained in vitro multistage antiplasmodium activity (ABS PfNF54 0.017 gametocytes PfiGc/PfLGc 1.24/1.39 μM, liver-stage PbHepG2 2.30 good microsomal metabolic stability (MLM CLint <...
UCT594 is a 2-aminopyrazine carboxylic acid
Abstract Africa’s vast genetic diversity poses challenges for optimising drug treatments in the continent, which is exacerbated by fact that discovery and development efforts have historically been performed outside Africa. This has led to suboptimal therapeutic outcomes African populations overall scarcity of relevant pharmacogenetic data, including characteristic genotypes as well drugs prescribed continent treat infectious diseases. Here, we propose a general approach identify drug-gene...
Background: The high incidence of adverse drug reactions (ADRs) in children is global concern. Enhancing the reporting ADRs could contribute to making safer medicines available children. Aim: To assess parents’ awareness and their knowledge on procedures South Africa. Setting: African parents with online access. Method: A quantitative descriptive study was conducted based an anonymous voluntarily web-based self-administered questionnaire that distributed through Facebook® LinkedIn™ Results:...
A simple and sensitive ultra-performance liquid chromatography tandem mass spectrometry method has been developed validated for the analysis of cycloserine in patients' plasma. Using methanol, cyloserine propranolol (internal standard (IS)) was extracted from plasma by protein precipitation procedure. The chromatographic separation successfully achieved on Phenomenex KinetexTM PFP C18 (2.1 mm × 100 mm, 2.6 μm) reversed-phase column. Acidified with 0.1% formic acid, water acetonitrile were...
Background: Tenofovir disoproxil fumarate (TDF) has been associated with kidney tubulardys function and reduced renal function. Limited studies were performed in Europe Asia that related plasma tenofovir (TFV) concentration function; no such to date have on Africans. Objective: To investigate the correlation between certain markers HIV-infected women TDF antiretroviral therapy (ART).These also compared a HIV-uninfected control group. Methods: ( n = 30) TDF-based ART matched 30 controls...