A5056751743- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Medical Imaging Techniques and Applications
- Cell Image Analysis Techniques
- Advanced X-ray and CT Imaging
- Radiation Dose and Imaging
- Genomics and Chromatin Dynamics
- Near-Field Optical Microscopy
- RNA Interference and Gene Delivery
- Private Equity and Venture Capital
- Retinal Diseases and Treatments
- 3D Printing in Biomedical Research
- Climate Change Policy and Economics
- Genetics, Bioinformatics, and Biomedical Research
- Protein Interaction Studies and Fluorescence Analysis
- Protein Degradation and Inhibitors
- Analytical Chemistry and Chromatography
- State Capitalism and Financial Governance
- Optical Coherence Tomography Applications
Kirchhoff (Germany)
2014-2024
Johannes Gutenberg University Mainz
2017-2022
Heidelberg University
2014-2022
Institute of Molecular Biology
2015-2016
Siemens Healthcare (Germany)
2013-2016
EBS University of Business and Law
2014
Cells detect and adapt to hypoxic nutritional stress through immediate transcriptional, translational metabolic responses. The environmental effects of ischemia on chromatin nanostructure were investigated using single molecule localization microscopy DNA binding dyes acetylated histones, by the sensitivity digestion with DNAseI, fluorescence recovery after photobleaching (FRAP) core linker histones. Short-term oxygen nutrient deprivation cardiomyocyte cell line HL-1 induces a previously...
For receptor tyrosine kinases supramolecular organization on the cell membrane is critical for their function. Super-resolution fluorescence microscopy techniques have offered new opportunities analysis of single localization. Here, we analysed cluster formation epidermal growth factor variant III (EGFRvIII), a deletion which expressed in glioblastoma. The constitutively activated EGFRvIII cells with an egfr gene amplification and thought to enhance tumorigenic potential especially...
The structure of the cell nucleus higher organisms has become a major topic advanced light microscopy. So far, variety methods have been applied, including confocal laser scanning fluorescence microscopy, 4Pi, STED and localisation microscopy approaches, as well different types patterned illumination modulated either laterally (in object plane) or axially (along optical axis). Based on our experience, we discuss here some application perspectives Modulated Illumination Microscopy (MIM) its...
In this paper, we present the prototype of an ophthalmoscope that uses structured illumination microscopy (SIM) to enable super-resolved imaging human retina, and give first insights into clinical application possibilities. The SIM technique was applied build a lens eye as objective 'super-resolve' retina living human. our multidisciplinary collaboration, have adapted well-established in ophthalmology successfully imaged using significantly lower light intensity than state-of-the-art...
Abstract Methods, experimental setups and perspectives of three-dimensional deep view imaging microscopy cell or tissue samples are reported. Preliminary biophysical clinically relevant examples presented.
Photon counting detectors in computed tomography facilitate measurements of spectral distributions detected X-ray quanta discrete energy bins. Along with the dependency on wavelength and atomic number mass attenuation coefficient, this information allows for reconstruction CT images different material bases. Decomposition two materials is considered standard today's dual-energy techniques. With photon-counting decomposition more than becomes achievable. Efficient detection CT-typical spectra...
Photon counting detectors are expected to bring along various clinical benefits in CT imaging. Among the of these is their intrinsic spectral sensitivity that allows resolve incident X-ray spectrum. Their capability for multi-energy imaging enables material segmentation, but it also possible use information create fused gray-scale images with improved properties. We have developed and investigated an optimization method maximizes image contrast-to-noise ratio, making data recorded a detector...
The role of technological innovation in mitigating and adapting to climate change has received growing interest recent years. Scholars have used system (IS) frameworks grasp the interdependencies such complex settings. However, this literature somewhat neglected two IS features, namely: (1) financial framework (2) negative unintended feedback mechanisms. Although financing is usually mentioned, it rarely goes beyond venture capital (VC) as part an entrepreneurial support network. In paper,...
The energy resolving capabilities of Photon Counting Detectors (PCD) in Computed Tomography (CT) facilitate energy-sensitive measurements. provided image-information can be processed with Dual Energy and Multi algorithms. A research PCD-CT firstly allows acquiring images a close to clinical configuration both the X-ray tube CT-detector. In this study, two algorithms (Material Decomposition Virtual Non-Contrast-imaging (VNC)) are applied on data set acquired from an anesthetized rabbit...
Abstract The environmental effects of ischemia on chromatin nanostructure were evaluated using single molecule localisation microscopy (SMLM) DNA binding dyes. Short-term oxygen and nutrient deprivation (OND) the cardiomyocyte cell-line HL-1induces a previously undescribed architecture, consisting large, sparse voids interspersed between DNA-dense hollow helicoid structures order 40 to 700 nm in dimension. OND induced compaction is reversible, upon restitution normoxia nutrients, transiently...