A5101482915- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- HIV/AIDS Research and Interventions
- Infective Endocarditis Diagnosis and Management
- Trypanosoma species research and implications
- Science and Education Research
- Antibiotic Use and Resistance
- Nosocomial Infections in ICU
- Antimicrobial Resistance in Staphylococcus
- Dermatological diseases and infestations
- Public Health in Brazil
- HIV/AIDS oral health manifestations
- Cytomegalovirus and herpesvirus research
- Antifungal resistance and susceptibility
- Sepsis Diagnosis and Treatment
- Health, Nursing, Elderly Care
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Disaster Response and Management
- Infections and bacterial resistance
- Syphilis Diagnosis and Treatment
- Clinical Laboratory Practices and Quality Control
Hospital Israelita Albert Einstein
2012-2022
Purdue University West Lafayette
2021
Michigan State University
2021
Cairo University
2021
Albert Einstein College of Medicine
2009-2012
Beneficência Portuguesa de São Paulo
2005-2010
Universidade de São Paulo
1990-2008
Hospital São Paulo
1999-2002
Hospital de Clínicas de Porto Alegre
2002
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
1992-1996
Healthcare-associated infections caused by Klebsiella pneumoniae isolates are increasing and few effective antibiotics currently available to treat patients. We observed decreased carbapenem susceptibility among K. isolated from patients at a tertiary private hospital that showed phenotype compatible with carbapenemase production although this group of enzymes was not detected in any sample. The aim study describe the epidemiology clinical outcomes associated carbapenem-resistant determine...
Abstract Background Complicated intra-abdominal infections (cIAI) remain challenging to treat because of their polymicrobial etiology including multi-drug resistant bacteria. The efficacy and safety tigecycline, an expanded broad-spectrum glycylcycline antibiotic, was compared with imipenem/cilastatin (IMI/CIS) in patients cIAI. Methods A prospective, double-blind, multinational trial conducted which cIAI randomly received intravenous (IV) tigecycline (100 mg initial dose, then 50 every 12...
We report an extensive, terbinafine-resistant (squalene epoxidase F397L mutation) Trichophyton indotineae infection in a previously healthy businessman from São Paulo, Brazil. The patient had traveled to France, Spain, and the United States. Clinician awareness, laboratory testing capacity, surveillance are essential prevent T. spread inform healthcare practices.
Abstract Introduction Deferred or inappropriate antibiotic treatment in ventilator-associated pneumonia (VAP) is associated with increased mortality, and clinical radiological criteria are frequently employed to establish an early diagnosis. Culture results used confirm the diagnosis adjust sometimes withdraw treatment. Tracheal aspirates have been shown be useful for these purposes. Nonetheless, little known about usefulness of quantitative findings tracheal secretions diagnosing VAP....
ABSTRACT Objective To investigate whether the urine dipstick screening test can be used to predict culture results. Methods A retrospective study conducted between January and December 2014 based on data from 8,587 patients with a medical order for test, sediment analysis culture. Sensitivity, specificity, positive negative predictive values were determined ROC curve was performed. Results The percentage of cultures 17.5%. Nitrite had 28% sensitivity 99% 89% 87%, respectively. Leukocyte...
COVID-19 patients on mechanical ventilation are at risk to develop invasive aspergillosis. To provide additional data regarding this intriguing entity, we conducted a retrospective study describing factors, radiology and prognosis of emerging entity in Brazilian referral centre.This included intubated (≥18 years) with admitted from April 2020 until July 2021 that had bronchoscopy investigate pulmonary co-infections. COVID-19-associated aspergillosis (CAPA) was defined according the European...
Rapid diagnosis of pathogens is decisive to guarantee adequate therapy in infections: culture methods are precise and sensitive, but rather slow. New resources available enable faster diagnosis, the most promising MALDI-TOF technology: mass spectrometry applied microbiological diagnosis. Times as fast 10 15 minutes etiological possible after a positive blood result. We hope have this technology our laboratory, ANVISA permitting improving their very slow rate doing things ... basically...
The collection of blood cultures is an extremely important method in the management patients with suspected infection. Microbiology laboratories should monitor culture collection.Over 8-month period we developed a prospective, observational study adult Intensive Care Unit (ICU). We correlated mass contained vials positivity and also verified relationship between volume collected for diagnosis bloodstream infection (BSI), as well explored factors predicting positive cultures.We evaluated 345...
Pseudomonas aeruginosa is an important opportunistic pathogen in hospitals, responsible for various infections that are difficult to treat due intrinsic and acquired antibiotic resistance. Here, 20 epidemiologically unrelated strains isolated from patients a general hospital over time period of two decades were analyzed using whole genome sequencing. The genomes compared order assess the presence predominant clone or sequence type (ST). No clonal structure was identified, but core...
Objective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well to compare the results with conventional blood culture.Methods A total of 114 consecutive patients clinical evidence sepsis were submitted culture LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) detected using than (17; 14.9%), an agreement 86.8%. Discordant seen in four only culture, ten one different pathogens found by each test. Infections...
Clostridium difficile is the major etiological agent of pseudomembranous colitis and found in up to 20% adult inpatients. The recommended treatment antibiotic therapy with metronidazole and/or vancomycin. However, recurrence rate may reach 25% it increases each episode. newest alternative treat diarrhea due recurrent fecal microbiota transplantation. procedure was performed 12 patients, a 6-month follow-up on 10 them. Of ten cases, bacterial diagnosed only one patient, after course urinary...
Com o declínio da taxa de infecções causadas pelo Haemophilus influenzae tipo b após a ampla introdução vacina, sorotipos não-b devem ser considerados agentes patogênicos potenciais em crianças menores 5 anos com doença invasiva. Relatamos um caso meningite por f lactente imunocompetente 1 ano. O agente foi identificado líquido cefalorraquidiano e hemocultura. A sorotipagem realizada testes soros policlonais confirmada reação cadeia polimerase. Todas as cepas associadas à invasiva deveriam...
Classical serological screening assays for Chagas' disease are time consuming and subjective. The objective of the present work is to evaluate enzyme immuno-assay (ELISA) methodology propose an algorithm blood banks be applied disease. Seven thousand, nine hundred ninety donor samples were screened by both reverse passive hemagglutination (RPHA) indirect immunofluorescence assay (IFA). Samples reactive on RPHA and/or IFA submitted supplementary RPHA, complement fixation (CFA) tests. This...
Automated instruments offer many advantages for clinical laboratories. Nevertheless, they can have problems identifying and determining susceptibilities of some pathogens. Vitek® 2 (bioMérieux) is an automated system that was recently introduced to Brazil. We evaluated the performance this equipment Brazilian isolates had been characterized using reference identification antimicrobial susceptibility testing methods. Ninety-nine strains Gram-positive cocci from a local center collection were...
Stenotrophomonas ssp. has a wide environmental distribution and is also found as an opportunistic pathogen, causing nosocomial or community-acquired infections. One species, S. maltophilia , presents multidrug resistance been associated with serious infections in pediatric immunocompromised patients. Therefore, it relevant to conduct profile phylogenetic studies clinical isolates for identifying infection origins augmented pathogenic potential. Here, multilocus sequence typing was performed...
To describe a norovirus outbreak in Brazilian long-term care facility from July 8 to 29, 2005.In the first 48 72 hours after onset of symptoms inpatients and employees, main infection control strategies were staff education, emphasis on hand washing, implementing contact precautions up 48-72 resolution symptoms, complete cleaning rooms exclusion symptomatic employees work until their symptoms. Epidemiological clinical characteristics infections described based chart review.The incidence...