The recalcitrant nature of lignocellulosic biomass hinders efficient exploitation this fraction for energy production. A better understanding the microorganisms able to convert plant-based feedstocks is needed improve anaerobic digestion biomass. In study, active thermophilic cellulose-degrading were identified from a full-scale digester fed with maize by using metagenome-resolved protein stable isotope probing (protein-SIP). 13C-cellulose was converted into 13C-methane 13/12C ratio 0.127...

The storage of liquid manure (slurry) is a major source methane (CH4) and thus contributes significantly to the climate impact agriculture. necessity store slurry in barns tanks at different seasons has led increasing research mitigation CH4 emissions from management chain. In this review, holistic view strategies targeting pits classified based on mechanism interaction (physical, chemical, biological) with their efficiency presented. Also, combination chemical additives other methods...

Excreta from housed animals are typically stored before land application, and storage is an important point source of CH4, N gases, odor. This study explored acidification as a strategy for greenhouse gas (GHG) mitigation, specifically the potential to reduce acid dose improve cost-effectiveness minimize environmental impacts. Pig slurry was with five doses concentrated sulfuric [1.2–6.0 kg (m3 slurry)−1] 63 days. Emissions CH4 NH3 were reduced by 46–96% 33–78%, respectively, increase in...

Reducing methane from livestock slurry is one of the quickest ways to counteract global warming. A straightforward strategy reduce retention time inside pig houses by frequent transfer outside storages, where temperature and therefore microbial activity are lower. We demonstrate three removal strategies in a year-round continuous measurement campaign. Slurry funnels, trays, weekly flushing reduced emission 89, 81, 53%, respectively. funnels trays ammonia 25–30%. An extended version anaerobic...

Gaseous emissions from livestock production are complex mixtures including ammonia, methane, volatile organic compounds (VOC), and H2S. These contribute to eutrophication, reduced air quality, global warming, odor nuisance. It is imperative that these gases mitigated in an environmentally sustainable manner. We present the discovery of a microbial inhibitor combo consisting tannic acid sodium fluoride (TA-NaF), which exhibits clear synergistic inhibition ammonia pure bacteria culture pig...

Methane (CH 4 ) emission from pig slurry is a large contributor to the climate footprint of livestock production. Acidification excreta animals with sulfuric acid, reduce CH and practiced at many Danish farms. Possible interaction effects other acidic agents or management practices (e.g. frequent removal residual acidification) have not been fully investigated. Here we assessed effect acidification range organic inorganic acids respect their inhibitor potential in several batch experiments...

Animal manure is a significant source of ammonia, methane, hydrogen sulfide, and odor emissions. Manure acidification to pH 5.5 using sulfuric acid (H2SO4) an approved method for reducing ammonia methane emissions from barns retaining bioavailable nitrogen in the slurry subsequent field fertilization. However, use H2SO4 may increase emission malodorous sulfur compounds reduces biogas production. In this study, we investigated if substituting part treatment with acetic (CH3COOH) improves two...

Reduced sulfur compounds emitted from livestock production cause odor nuisance for local residents. The microbial processes responsible this are not well described in swine manure and a method monitoring the biological is necessary to develop strategic abatement technologies. In study, Proton-Transfer-Reaction Mass Spectrometry isotope-labeled sulfate were combined applied elucidate with high time resolution. We successfully monitored reduction of isotope 33S labeled into corresponding...

Germicidal ultraviolet lamps with a peak emission at 222 nm (GUV222) are gaining prominence as safe and effective solution to reduce disease transmission in occupied indoor environments. While previous studies have reported O

Carbon isotope ratio analysis using cavity ring-down spectroscopy (CRDS) offers insight into methanogenic activity. In livestock buildings, measuring 13 CH4 /12 by CRDS is challenging and prone to errors, due cross-interference from NH3 . Therefore, an interference-corrected improved approach monitor pathways with was developed described in this study.Cross-interference other gases on measured CRDS. The removal efficiency of quantified proton transfer reaction mass spectrometry. linearity...

Liquid manure (slurry) from livestock releases methane (CH4) that contributes significantly to global warming. Existing models for slurry CH4 production-used mitigation and inventories-include effects of organic matter loading, temperature, retention time but cannot predict important management, or adequately capture essential temperature-driven dynamics. Here we present a new model includes multiple methanogenic groups whose relative abundance shifts in response changes temperature other...

Reducing methane (CH4) emission from livestock production can be achieved by the treatment of manure in slurry pits and storage tanks. In case pits, treating residual left out after frequent flushing largely reduce CH4 due to decrease methanogenic inoculum. this study, three surfactants, two oxidants a flocculant were screened for their efficiency reducing pig headspace setup. All surfactants reduced > 95% at dosage 8 g kg−1 particularly sodium dodecyl sulfate (SDS) was effective low only...

Manure management is a significant source of global methane emissions, and there an increased interest in understanding predicting emissions. The hydrolysis rate manure organic matter critical for We estimated constants crude protein, fibers, lipids used the Arrhenius equation to describe its dependency on temperature. Simultaneously, measurements emission, 13/12 C isotope ratios, methanogen community were conducted. This was achieved by incubating fresh pig without inoculum at 10°C, 15°C,...

Greenhouse gas emission from liquid livestock manure storage is a considerable contributor to global warming and accurate farm-scale models for predicting are needed estimating effects of management strategies. In this study we measured degradation organic matter components pig slurry with anaerobic aerobic surface at 10℃ 20℃. Simultaneously, methane carbon dioxide were both processes was determined. Carbon loss due respiration, did not limit during the incubation...

Greenhouse gas emissions from liquid livestock manure storage significantly contribute to global warming. Accurate farm-scale models are essential for predicting these and evaluating management strategies, but they rely on multiple parameters describing carbon loss dynamics. Surface respiration may influence methane emission, yet it is not explicitly included in current models. We conducted experiments measure pig surface rate its effect organic matter degradation dioxide emissions. Manure...

Livestock manure emits reduced sulfur compounds and methane, which affect nature the climate. These gases are efficiently mitigated by addition of a tannic acid-sodium fluoride combination inhibitor (TA-NaF), to some extent acidification. In this paper, TA-NaF treatment was performed on swine study influence methanogenic pathways transformation in various laboratory experiments. Stable carbon isotope labeling revealed that both untreated treated manures were dominated hydrogenotrophic...