Stem cells have recently been isolated from humans and mice but not rat tendon tissue. This study reports the isolation characterization of stem tendon. Nucleated flexor tissues after collagenase digestion were plated at a low cell density to allow selective proliferation tendon-derived cells. About 1-2% under this optimized culturing condition showed clonogenicity, high proliferative potential seeding density, osteogenic, chondrogenic, adipogenic multidifferentiation potential. These...

Abstract Injured tendons heal slowly and often result in the formation of mechanically functionally inferior fibrotic scar tissue or fibrous adhesions. This study investigated use tendon‐derived stem cells (TDSCs) for tendon repair a rat patellar window defect model. Fibrin glue constructs with without GFP‐TDSCs were transplanted into defect. The harvested histology, ex vivo fluorescent imaging biomechanical test at various time points up to week 4. Our results showed that TDSCs...

The use of tendon-derived stem cells (TDSCs) as a cell source for musculoskeletal tissue engineering has not been compared with that bone marrow stromal (BMSC). This study the mesenchymal (MSC) and embryonic (ESC) markers, clonogenicity, proliferative capacity, multilineage differentiation potential rat TDSC BMSC in vitro. MSC ESC marker profiles paired were using flow cytometry quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Their clonogenicity capacity...

Abstract This study aimed to investigate the effect of repetitive tensile loading on expression BMP‐2 and osteogenic differentiation tendon‐derived stem cells (TDSCs) in vitro. Repetitive stretching was applied TDSCs isolated from rat patellar tendon at 0%, 4%, 8%, 0.5 Hz. The detected by Western blotting qPCR. To effects TDSCs, added TDSC monolayer for detection ALP activity calcium nodule formation a separate experiment. adhered, proliferated, aligned along direction externally force while...

Surgical reattachment of tendon and bone such as in rotator cuff repair, patellar-patella repair anterior cruciate ligament (ACL) reconstruction often fails due to the failure regeneration specialized tissue ("enthesis") which connects bone. Tendon-to-bone healing taking place between inhomogenous tissues is a slow process compared within homogenous tissue, or healing. Therefore special attention must be paid augment insertion (TBI) Apart from surgical fixation, biological biophysical...

Our understanding of the pathogenesis "tendinopathy" is based on fragmented evidences like pieces a jigsaw puzzle. We propose "failed healing theory" to knit these fragments together, which can explain previous observations. also that albeit "overuse injury" and other insidious "micro trauma" may well be primary triggers process, not an per se. The typical clinical, histological biochemical presentation relates localized chronic pain condition lead tendon rupture, latter attributed...

Background: Both osteointegration and remodeling of graft midsubstance (collectively called healing) are slow processes after anterior cruciate ligament (ACL) reconstruction. Tendon-derived stem cells (TDSCs) form a cell sheet treatment with connective tissue growth factor (CTGF) ascorbic acid, which exhibits higher tenogenic maintains high chondro-osteogenic gene expression TDSCs. No external scaffold is required for delivery. Hypothesis: Wrapping the TDSC around ACL would promote early...

This study aimed to compare clonogenicity, proliferation, stem cell-related marker expression, senescence, and differentiation potential of rat patellar tendon-derived cells (TDSCs) at early (P5), mid (P10), late (P20, P30) passages. The clonogenicity the was assessed by colony-forming assay their proliferative bromodeoxyuridine assay. surface expression CD90 CD73 flow cytometry. cellular senescence β-galactosidase activity. adipogenic, chondrogenic, osteogenic potentials TDSCs were standard...

We hypothesized that altered fate of tendon-derived stem cells (TDSCs) might contribute to chondro-ossification and failed healing in the collagenase-induced (CI) tendon injury model. This study aimed compare yield, proliferative capacity, immunophenotypes, senescence, differentiation potential TDSCs isolated from healthy (HT) CI tendons. were Sprague-Dawley rat patellar The senescence compared by colony-forming unit assay, BrdU flow cytometry, β-galactosidase activity respectively. Their...

We investigated the spatial distribution of stem cells in tendons and roles early tendon repair. The relationship between tendon-derived (TDSCs) isolated vitro vivo was also explored. Iododeoxyuridine (IdU) label-retaining method used for labeling rat patellar with without injury. Co-localization (LRCs) different markers done by immunofluorescent staining. TDSCs were from mid-substance after IdU pulsing, expression fresh expanded More LRCs found at peritenon tendon-bone junction compared...

Chronic tendinopathy is a tendon disorder that common in athletes and individuals whose tendons are subjected to repetitive strain injuries. The presence of ossification worsened the clinical manifestation disorder. change loading due mechanical overload, compression, or disuse have been implicated as possible etiologies, but pathological mechanisms remain unclear. In this study, we demonstrated tissue might be osteogenesis tendon-derived stem cells (TDSCs) induced by uniaxial tension (UMT)...

Excessive inflammation contributes to the pathogenesis of tendinopathy. Fatty acid binding protein 4 (FABP4) is a pro-inflammatory adipokine mediating various metabolic and inflammatory diseases. This study aimed examine expression FABP4 its association with expressions cytokines in The effects single injection on tendon pathology were examined. effect IL-1β tendon-derived stem/progenitor cells (TDSCs) also investigated.

We report chondrocyte phenotype and ectopic ossification in a collagenase-induced patellar tendon injury model. Collagenase or saline was injected intratendinously one limb. The patella harvested for assessment at different times. There an increase cellularity, vascularity, loss of matrix organization with time after collagenase injection. did not heal histologically until week 32. Ectopic mineralization as indicated by von Kossa staining started from 8. Tendon calcification mediated...

Alteration in the composition of extracellular matrix has been suggested as major factor for development tendinopathy and calcified tendinopathy, which poorer clinical manifestation. This study investigated changes proteoglycans collagens a model correlated expression with acquisition chondrocyte phenotype, ectopic ossification loss organization same model.Thirty-six rats were used. Collagenase or saline was injected into patellar tendons each rat. At Weeks 2, 4 12, samples used...

Tendons regenerate and repair slowly inefficiently after injury. Tendon-derived stem cells (TDSCs) have been isolated recently shown to promote tendon repair. The ability achieve sufficient numbers of for transplantation is essential their clinical application. In this study, we aimed study the effect low oxygen (O(2)) tension (2%) on clonogenicity, metabolic rate, DNA incorporation, population doubling time, β-galactosidase activity, immunophenotypes, multilineage differentiation potential,...