A5019766762- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Genetic factors in colorectal cancer
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- PARP inhibition in cancer therapy
- Signaling Pathways in Disease
- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Fibrosis and Remodeling
- Endoplasmic Reticulum Stress and Disease
- Animal Genetics and Reproduction
- Biochemical and Molecular Research
IFOM
2016-2024
University of Milan
2022-2024
Magna Graecia University
2011
POLθ promotes repair of DNA double-strand breaks (DSBs) resulting from collapsed forks in homologous recombination (HR) defective tumors. Inactivation results synthetic lethality with the loss HR genes BRCA1/2, which induces under-replicated accumulation. However, it is unclear whether POLθ-dependent replication prevents HR-deficiency-associated lethality. Here, we isolated Xenopus laevis and showed that processes stalled Okazaki fragments, directly visualized by electron microscopy, thereby...
Ferritin, the major intracellular iron-storage protein, is made of 24 subunits two types, H and L. Besides regulating iron homeostasis, it has been found that ferritin, in particular subunit (FHC), involved different biological events such as cell differentiation pathologic states (i.e., neurodegeneration cancer). This study aimed at investigating whole-cell proteome FHC-expressing sh-RNA-silenced human metastatic melanoma cells (MM07m) attempt to identify classify highest number proteins...
Abstract In several metazoans, the number of active replication origins in embryonic nuclei is higher than somatic ones, ensuring rapid genome duplication during synchronous cell divisions. High origin density can be restored by nuclear reprogramming. However, mechanisms underlying high formation coupled to cycles are poorly understood. Here, using Xenopus laevis , we show that SSRP1 stimulates assembly on chromatin promoting eviction histone H1 through its N-terminal domain. Histone removal...
Replication origin assembly is a pivotal step in chromosomal DNA replication. In this process, the ORC complex binds and, together with CDC6 and CDT1, promotes loading of MCM helicase. Chemicals targeting might be useful to sensitize highly proliferative cancer cells. However, identifying such compounds challenging due multistage nature process. Here, using
Abstract During early embryonic development in several metazoans, accurate DNA replication is ensured by high number of origins. This guarantees rapid genome duplication coordinated with fast cell divisions. In Xenopus laevis embryos this program switches to one a lower origins at developmental stage known as mid‐blastula transition (MBT) when cycle length increases and gene transcription starts. Consistent regulation, somatic nuclei replicate poorly transferred eggs, suggesting the...