The core fucosylation of N-glycans on glycoproteins is catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and involved various biological functions, such as protein function, cancer progression, postnatal development. substrate specificity FUT8 toward bi-antennary has been reported, but it unclear with regard to tri-antennary tetra-antennary glycans. Here, we examined the activity human tri- forms glycopeptides. We found that glycan [A3(2,4,2) type] terminated N-acetylglucosamine...

Modulation of N-glycosylation using human Golgi α-mannosidase II (α-hGMII) inhibitors is a potential anticancer approach, but the clinical utility current α-hGMII limited by their co-inhibition lysosomal (α-hLM), resulting in abnormal storage oligomannoses. We describe synthesis and screening small library novel bicyclic iminosugar-based scaffolds, prepared via natural product-inspired combinatorial chemistry (NPICC), which resulted identification primary inhibitor with 13.5-fold selectivity...

Up to now, all commercial matrix-assisted laser desorption/ionization (MALDI) mass spectrometers still can not efficiently analyze very large biomolecules. In this work, we report the development of a novel MALDI ion trap spectrometer which enrich biomolecular ions enhance detection sensitivity. A charge detector was installed measure directly. With design, first measurement IgM with mass-to-charge ratio (m/z) at 980 000. addition, quantitative measurements number be obtained. step function...

Abstract We used pseudo‐triple‐stage tandem MS to rapidly differentiate terminal α(2,3)‐ and α(2,6)‐linked sialic acids of glycopeptides native glycans. The precursor ions generate important diagnostic fragments distinguish different linked isomeric glycans based on collision‐induced dissociation (CID). include hexose with N ‐acetylhexosamine residues, acid the loss water, residues. Their values mass‐to‐charge ratio ( m / z ) are at 366.14, 274.09, 292.10, respectively. relative intensity...

Sonic spray ionization (SSI) has been explored as an ambient method for mass spectrometric analysis of different compounds.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrohydrodynamic mass spectrometric studies of some polyether-cation complexesVictor F. Man, J. Dale Lin, and Kelsey D. CookCite this: Am. Chem. Soc. 1985, 107, 16, 4635–4640Publication Date (Print):August 1, 1985Publication History Published online1 May 2002Published inissue 1 August 1985https://pubs.acs.org/doi/10.1021/ja00302a006https://doi.org/10.1021/ja00302a006research-articleACS PublicationsRequest reuse permissionsArticle...

A novel self-powered dual spray ionization source has been developed for applications in mass spectrometry. This new does not use any power supply and produces both positive negative ions simultaneously. The idea behind this comes from the Kelvin water dropper. employs one or two syringes, pneumatic sprays operated over a range of flow rates (0.15–15 μL min−1) gas pressures (0–150 psi), double layered metal screens ion formation. variable electrostatic potential 0 to 4 kV can be produced...

Until now, there have been no relatively easy methods to measure the mass and distributions of nanoparticles/viruses. In this work, we report first set measurements for nanoparticles/viruses using a novel spectrometry technology. past, was typically used particle or molecule with less than 1,000,000 Da. We developed cell that can microparticle. Nevertheless, is gap measurement in region nanoparticle virus (1 MDa 1 GDa). Here, nanoparticle/virus technique make rapid accurate distribution This...

An ESI ion trap mass spectrometer was designed for high-throughput and rapid analysis of large bioparticles. Mass calibration the instrument performed using commercially available polystyrene (PS) microparticles with a size comparable to cancer cells. Different sizes MCF-7 breast cells (8 15 μm) were used in this study. The masses different measured. This system allows all types particles.

In the past, matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI), used for large biomolecule detection, were usually installed in two separate mass spectrometers.

Insulin is a peptide hormone secreted by pancreatic β-cells. Ca(II) and Mg(II) ions play an important role in the secretion of insulin. There no study about direct complexation or with insulin their equilibrium constants. Electrospray ionization mass spectrometry (ESI-MS) practical method for monitoring non-covalent complexes such as Ca(II)-insulin Mg(II)-insulin. Here, constants Mg(II)-insulin have been calculated after ESI-MS measurements aqueous solutions.The effects pH, competitive...

Due to the heterogeneous and isomeric nature of glycans, development an advanced separation distinct glycan isomers is essential for research application. In this study, we utilized porous graphite carbon (PGC) chromatography oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven. Furthermore, fine structures could be identified by using ultrahigh temperature PGC liquid mass spectrometry (UHT-PGC-LCMS). A nonreduced hydrolyzed dextran was...

To find their potential use in protein research, direct addition of a disulfide compound to alkyne (namely disulfide–yne reaction) and S-arylation with arenediazonium salt disulfide–diazonium were investigated aqueous or protic solutions. The reaction dimethyl 5-hexynol performed best under 300 nm irradiation the presence sodium acetate afford 5,6-bis(methylthio)-5-hexenol 60% yield. Without prior reduction bond thiols, reactions have advantage 100% atom economy. Disulfide–diazonium was...

In this work, we report a new design of charge detection quadrupole ion trap mass spectrometer (QIT-MS) for the analysis micro-sized dry inorganic and bioparticles including red blood cells (RBCs) different sizes MCF-7 breast cancer cells. The developed method is one fastest methods to measure particles. This system allows online various particles up 1 × 1017 Da. calibration has been done by using polystyrene (PS) (2-15 μm). measured masses RBCs were around 1.8 1013 Da between 1014 4...

In this work, we present the detection sensitivity improvement of electrospray ionization (ESI) mass spectrometry neutral saccharides in a positive ion mode by addition various amino acids. Saccharides broad molecular weight range were chosen as model compounds study. provide strong noncovalent interactions with acids, and complex formation enhances signal intensity simplifies spectra saccharides. Polysaccharides polymer-like ESI spectrum basic subunit difference between multiply charged...

Recently, mass spectrometry has been extended to detect large organic particles and biomolecules, which include protein polymers, nanoparticles, virions, microparticles cells. Different novel technologies have developed these very particles. In this review, a brief introduction the technology development is given, future perspectives on applications are included. terms of detection macromolecular ion accelerator was achieve sufficient molecular energy reach megavolt region increase...

Presented herein are the development of macromolecular ion accelerator (MIA) and results obtained by MIA. This new instrument utilizes a consecutive series planar electrodes for purpose facilitating stepwise acceleration. Matrix-assisted laser desorption/ionization (MALDI) is employed to generate singly charged ions. A regular Z-gap microchannel plate (MCP) detector mounted at end record signals. In this work, we demonstrated detection ions with mass-to-charge (m/z) ratio reaching...

Human serum is one of the most attractive specimens in biomarker research. However, its overcomplicated properties have hindered analysis low-abundance proteins by conventional mass spectrometry techniques. This work proposes an innovative strategy for utilizing nanodiamonds (NDs) combination with Triton X-114 protein extraction to fractionate crude six pH-tuned fractions, simplifying overall proteome and facilitating profiling high efficiency. A total 663 are identified evenly distributed...

We developed the first dual-ion-trap mass analyzer which can detect ions with a high mass-to-charge ratio (m/z > 6000). The ion trap is quadrupole (QIT), was operated by step scanning of trapping frequency for sample containing mixtures biomolecules. second trap, linear (LIT), utilized to capture selected ejected out QIT so that all from be examined one one. It found transferred LIT ∼60% efficiency large biomolecular m/z. This far highest transfer in dual device high-mass ions.