A5081244647- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
- Congenital Heart Disease Studies
- Hemispheric Asymmetry in Neuroscience
- Cardiac Fibrosis and Remodeling
- Cell Adhesion Molecules Research
- TGF-β signaling in diseases
- Estrogen and related hormone effects
- Pluripotent Stem Cells Research
- Gene expression and cancer classification
- Cancer-related Molecular Pathways
- Marine Toxins and Detection Methods
- Tissue Engineering and Regenerative Medicine
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Coronary Artery Anomalies
- Cardiomyopathy and Myosin Studies
- Planarian Biology and Electrostimulation
- Bioinformatics and Genomic Networks
- Zebrafish Biomedical Research Applications
- Hedgehog Signaling Pathway Studies
- Reproductive Biology and Fertility
- Nicotinic Acetylcholine Receptors Study
University of South Carolina
2004-2019
Medical University of South Carolina
1997-2019
MUSC Hollings Cancer Center
2016-2019
Center for Children
1998-1999
University of Utah
1998-1999
Huntsman Cancer Institute
1998-1999
Abstract The rightward looping of the primary heart tube is dependent upon upstream patterning events that establish vertebrate left-right axis. In Xenopus, a left-sided Vg1 signaling pathway has been implicated in instructing cells to adopt ‘left-sided identity’; however, it not known whether ‘right-sided identity’ acquired by default or antagonism signaling. Here, we propose an antagonistic, BMP/ALK2/Smad-mediated active on right side Xenopus embryo. Truncated ALK2 receptor expression...
Left–right asymmetry is a phenomenon that has broad appeal—to anatomists, developmental biologists and evolutionary biologists—because it morphological feature of organisms spans scales size levels organization, from unicellular protists, to vertebrate organs, social behaviour. Here, we highlight number important aspects encompass several areas biology—cell-level, physiological, genetic, anatomical components—and are based on research conducted in diverse model systems, ranging single cells...
The significant morbidity and mortality associated with laterality disease almost always are attributed to complex congenital heart defects (CHDs), reflecting the extreme susceptibility of developing disturbances in left-right (LR) body plan. To determine how LR positional information becomes ;translated' into anatomical asymmetry, left versus right side cardiomyocyte cell lineages were traced normal defective embryos frog, Xenopus laevis. In embryos, myocytes some regions derived...
Background: Low success rates in oncology drug development are prompting re-evaluation of preclinical models, including orthotopic tumor engraftment. In breast cancer cells typically injected into mouse mammary fat pads (MFP). However, this approach bypasses the epithelial microenvironment, potentially altering properties ways that affect translational application. Materials and Methods: Tumors were generated by intraductal (MIND) engraftment 4T1 carcinoma cells. Growth, histopathology,...
WNT signaling has been shown to influence the development of heart. Although recent data suggested that canonical WNTs promote emergence and expansion cardiac progenitors in pregastrula embryo, it long accepted once gastrulation begins, needs be suppressed for proceed. Yet, this latter supposition appears odds with expression multiple developing The present study examining effect ectopic on cardiogenesis frog was designed test hypothesis heart formation is dependent inhibition activity at...
Endocardial cushion tissue is formed by an epithelial-mesenchymal transformation of endocardial cells, a process which results from inductive interaction between the myocardium and endocardium within atrioventricular (AV) outflow tract (OT) regions heart. We report here that protein previously found to be required for myocardially induced cells in vitro, ES/130, highly expressed AV OT not only myocardial but also its mesenchymal progeny. Given these findings others, we have tested hypothesis...
Lithium is a commonly used drug for the treatment of bipolar disorder. At high doses, lithium becomes teratogenic, which property that has allowed this agent to serve as useful tool dissecting molecular pathways regulate embryogenesis. This study was designed examine impact on heart formation in developing frog insights into regulation cardiac specification. Embryos were exposed at beginning gastrulation, produced severe malformations anterior end embryo. Although previous reports...
The majority of complex congenital heart defects occur in individuals who are afflicted by laterality disease. We hypothesize that the prevalence valvuloseptal this population is due to defective left-right patterning embryonic atrioventricular (AV) canal cushions, which progenitor tissue for valve and septal structures mature heart. Using embryos frog Xenopus laevis, hypothesis was tested performing lineage analysis myocytes cushion mesenchyme cells superior inferior regions AV canal....
Left–right (L–R) differences in mammographic parenchymal patterns are an early predictor of breast cancer risk; however, the basis for this asymmetry is unknown. Here, we use retinoid X receptor alpha heterozygous null (RXRα +/− ) mice to propose a developmental origin: perturbation coordinated anterior–posterior (A–P) and L–R axial body patterning. We hypothesized that by analogy somitogenesis—in which retinoic acid (RA) attenuation causes anterior somite pairs develop asynchronously—that...
Abstract The type I transforming growth factor‐beta (TGFβ) receptor, activin‐like kinase‐4 (ALK4), is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left–right axis determination. To complement previous ALK4 functional studies, we have analyzed expression embryos the frog, Xenopus laevis . Results obtained reverse transcriptase‐polymerase chain reaction indicate that present both...
Heterotaxy syndrome is characterized by abnormal thoracic and/or visceral organ left-right (LR) asymmetry. The significant morbidity and mortality associated with heterotaxy almost always are attributed to complex cardiac defects, reflecting the extreme susceptibility of developing heart disturbances in LR body plan. To determine how positional information becomes “translated” into anatomical asymmetry, we used vivo markers trace left (L) vs. right (R) side cardiomyocyte lineages embryos...