A5032001907- Cell death mechanisms and regulation
- interferon and immune responses
- Inflammasome and immune disorders
- Viral Infections and Outbreaks Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Streptococcal Infections and Treatments
- Sphingolipid Metabolism and Signaling
- Antimicrobial Resistance in Staphylococcus
- Phagocytosis and Immune Regulation
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Virus-based gene therapy research
- Immune Response and Inflammation
- Viral gastroenteritis research and epidemiology
- Heat shock proteins research
- Ocular Infections and Treatments
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Cutaneous lymphoproliferative disorders research
- Biochemical and Molecular Research
- SARS-CoV-2 and COVID-19 Research
- Congenital gastrointestinal and neural anomalies
- Misinformation and Its Impacts
- Calpain Protease Function and Regulation
South Dakota State University
2022-2025
Goethe University Frankfurt
2012-2023
University Hospital Frankfurt
2014-2021
Johannes Gutenberg University Mainz
2017
University Medical Center
2017
Institute of Immunology
2017
University Medical Center of the Johannes Gutenberg University Mainz
2017
Hungarian Academy of Sciences
2015
Goethe Institute
2012
Semmelweis University
1999-2007
Activation of nuclear factor-κB (NF-κB) signaling was observed in pancreatic adenocarcinoma cell lines and tumours. However, information on the expression RelA/p65, major transcription activating NF-κB subunit, these carcinomas possible correlations thereof with activation patient survival is not available. To provide this missing translational link, we analysed RelA/p65 82 adenocarcinomas by immunohistochemistry. Moreover, measured pathway 11 tumours quantitative PCR for target genes. We...
For a long time necrosis was thought to be an uncontrolled process but evidences recently have revealed that can also occur in regulated manner. Necroptosis, type of programmed is defined as death receptor-initiated under caspase-compromised conditions. The requires the kinase activity receptor-interacting protein 1 and 3 (RIPK1 RIPK3) mixed lineage domain-like (MLKL), substrate RIPK3. further downstream events remain elusive. We applied known inhibitors characterize contributing enzymes...
When investigating cellular responses to viral infections, there is a need quantitatively distinguish between dying cells, infected and cells that are both dying. Here, we present protocol simultaneously detect porcine reproductive respiratory syndrome virus (PRRSV) infection cell death using flow cytometry double-staining approach. We describe steps for seeding, infection, collection; staining dead cells; sample fixation; permeabilization. then detail procedures immunostaining analysis.
Caspase-2 represents the most conserved member of caspase family, which exhibits features both initiator and effector caspases. Using ribonucleoprotein (RNP)-immunoprecipitation assay, we identified proapoptotic caspase-2L encoding mRNA as a novel target ubiquitous RNA-binding protein HuR in DLD-1 colon carcinoma cells. Unexpectedly, crosslinking-RNP RNA probe pull-down experiments revealed that binds exclusively to caspase-2-5′ untranslated region (UTR) despite 3′ UTR bears several...
Cutaneous T cell lymphomas (CTCLs) represent a heterogeneous group of that primarily affect the skin. The most frequent forms CTCL are mycosis fungoides and Sézary syndrome. Both characterized by recurrence, developing chronic conditions high mortality with lack curative treatment. In this study, we evaluated effect short-chain, cell-permeable C6 Ceramide (C6Cer) on lines keratinocytes. C6Cer significantly reduced viability induced death via apoptosis necrosis. contrast, primary human...
Caspase-2 represents an evolutionary conserved caspase, which plays a role in genotoxic stress-induced apoptosis, ageing-related metabolic changes, and deleting aneuploid cells tumors. Genetic deletion of caspase-2 leads to increased tumor susceptibility vivo. The exact downstream signaling mechanism by accomplishes its specific suppressor functions is not clear. Caspase-2, uniquely among caspases, resides the nucleus other cellular compartments. In this study, we identify nuclear substrate,...
The healing of wounded skin is a highly organized process involving massive cell in- and outflux, proliferation tissue remodelling. It well accepted that metabolic constraints such as diabetes mellitus, overweight or anorexia impairs wound healing. Indeed, inflammation involves boost overall changes. As converges inflammatory processes are also common to transformation, we investigate the functional role pro-neoplastic factor pyruvate kinase (PK) M2 its active splice variant PKM1 in...
Ebola virus (EBOV) is responsible for outbreaks with case fatality rates of up to 90% and an epidemic in West Africa more than ten thousand deaths. EBOV glycoprotein (EBOV-GP) the only viral surface protein entry into cells. Here, by employing pseudotyped EBOV-GP particles, we uncover a critical role sphingolipids inhibiting entry. Sphingosine kinase 1 (SphK1) catalyzes phosphorylation sphingosine 1-phosphate (S1P). The administration SphK1 activator, K6PC-5, or S1P, overexpression...
Therapy resistance is still a major reason for treatment failure in colorectal cancer (CRC). Previously, we identified the E3 ubiquitin ligase TRIM25 as novel suppressor of caspase-2 translation which contributes to apoptosis CRC cells towards chemotherapeutic drugs. Here, report executioner caspase-7 being further target TRIM25. The results from gain- and loss-of-function approaches actinomycin D experiments indicate that attenuates expression mainly through decrease mRNA stability. data...
Summary The avian bursa of Fabricius has a direct connection to the cloaca via bursal duct. Using duct ligation technique, it been clearly shown that B cells follicles develop under influence cloacal antigens. These antigens have suggested be present on secretory dendritic in immunoglobulin G (IgG)‐containing complexes. We studied effect maternal (yolk) early development and appearance IgG‐containing complexes with novel embryo manipulation ovo vitelline ligation. This operation blocked...
Experimental work on highly pathogenic viruses such as Ebola virus (EBOV) and severe acute respiratory syndrome coronavirus-2 requires high-level biosafety facilities. Here, we provide a detailed step-by-step protocol which details the production application of replication-incompetent murine leukemia virus-based pseudotyped particles to monitor quantify viral entry efficiency in human cell lines under level-2 conditions. We describe use encoding luciferase gene quantification transduction by...
Ebola virus (EBOV) is responsible for outbreaks with case-fatality rates of up to 90% and an epidemic in West Africa more than ten thousand deaths. EBOV glycoprotein (EBOV-GP) the only viral surface protein entry into cells. It has been suggested play a role cytopathic effects induced by virus. Here we uncover critical sphingolipids inhibiting virus-mediated cytotoxicity. Sphingosine kinase 1 (SphK1) catalyzes phosphorylation sphingosine sphingosine-1-phosphate (S1P). The administration...