Estrogens, including the natural hormones estrone (E1) and estradiol (E2), are thought to be involved in tumor induction. Catechol estrogen quinones (CEQ) derived from 4-hydroxyestrone (4-OHE1) 4-hydroxyestradiol (4-OHE2) react with DNA form depurinating N7Gua N3Ade adducts that might responsible for initiation (Cavalieri, E. L., et al. (2000) J. Natl. Cancer Inst. Monogr. 27, 75). Current detection limits CEQ-derived by high-performance liquid chromatography multichannel electrochemical...

BACKGROUND Catechol estrogen quinones (CEQ) derived from 4-hydroxyestrone (4-OHE1) and 4-hydroxyestradiol (4-OHE2) react with DNA to form depurinating—N7Gua and—N3Ade adducts. This damage leads mutations that can initiate breast prostate cancer. To determine whether this occurs in humans, urine samples men cancer benign urological conditions, healthy controls were analyzed. The objective was determining any of the patients had formed depurinating 4-OHE1(E2)-1-N3Ade METHODS adducts extracted...

We perform multi-class classification of laser-induced breakdown spectroscopy data four commercial samples proteins diluted in phosphate-buffered saline solution at different concentrations: bovine serum albumin, osteopontin, leptin, and insulin-like growth factor II. achieve this by using principal component analysis as a method for dimensionality reduction. In addition, we apply several algorithms (K-nearest neighbor, regression trees, neural networks, support vector machines, adaptive...

The aim of this work was to study the applicability infrared spectroscopy combined with machine learning techniques evaluate uptake and distribution gold nanoparticles (AuNPs) single-walled carbon nanotubes (CNTs) in Cicer arietinum L. (chickpea). Obtained spectral data revealed that AuNPs CNTs by C. seedlings' root resulted accumulation at stem leaf parts, which consequently led heterogeneous nanoparticles. principal component analysis support vector classification were applied assess its...

In recent years, the spectroscopy community has increasingly been using various techniques for automatic computer assisted quantitative and qualitative evaluation of specimen based on data. this paper, an automated method classification four types proteins (Bovine Serum Albumin (the most abundant protein in blood plasma), Osteopontin, Leptin Insulin-like Growth Factor II) (identified as potential biomarkers ovarian cancer) from laser-induced breakdown (LIBS) data is proposed. Automatic these...

The green pea (Pisum sativum L.) is an economically, nutritionally, and culturally important legume. It a crop that subject to various investigations due its popularity with the development of protocols in different topics, except for nano-biotechnological studies. This work was carried out evaluate uptake, distribution, translocation, bioaccumulation single-walled carbon nanotubes (CNTs) gold nanoparticles (AuNPs) within economically plant Pisum morphologically anatomically dark-field...

We report on a new application of laser induced breakdown spectroscopy (LIBS) for the diagnosis diseases such as ovarian cancer. perform detection cancer biomarker CA 125 based LIBS measurements. Immunoconjugated Silicon particles are incubated with affinity agarose beads carrying CA125 molecules. In competitive method Si IgG molecules pre-incubated CA125. This pre-incubation decreases numbers free available consequent interaction beads. Thus less attached to and consequently smaller peak...

We report on pump-probe mode-mismatched photothermal lens experiments of metallic micro- and nanoparticle water solutions. show that colloids exhibit nonlinear absorption effects related to the forces result from interaction with electromagnetic radiation. The a double peak Z-scan shape is associated presence attraction when irradiating at 400 nm. estimate corresponding coefficients using Fresnel diffraction approximation for description signal.

Jensen-Shannon divergence is an effective method for measuring the distance between two probability distributions. When difference these distributions subtle, does not provide adequate separation to draw distinctions from subtly different We extend by reformulating it using alternate operators that properties concerning robustness. Furthermore, we prove a number of important this extension: lower limits its range, and relationship Shannon Entropy Kullback-Leibler divergence. Finally, propose...

We report on the quantitative identification of hydrogen composition high molecular weight proteins in a heavy water solution by laser-induced breakdown spectroscopy.

We investigate performance of neural networks for classification laser-induced breakdown spectroscopic data four proteins: Bovine Serum Albumin, Osteopontin, Leptin and Insulin-like Growth Factor II. utilize principal component analysis algorithm feature extraction multilayer perceptrons algorithms with one two hidden layers. employ leave-one-out procedure classifier evaluation. Our experimental results indicate that methods linear convergence can provide accuracy superior to quadratic convergence.

Catechol estrogen quinones (CEQ) derived from oxidation of the catechol estrogens 4-hydroxyestrone (4-OHE1) and 4-hydroxyestradiol (4-OHE2) can conjugate with glutathione (GSH), a reaction that prevents damage to DNA provide biomarkers exposure CEQs. Monoclonal antibodies (MAb) 4-OHE1(E2)-2-N-acetylcysteine [4-OHE1(E2)-2-NAcCys] were developed characterized by immunological spectroscopic studies. The NAcCys is hydrolytic product corresponding GSH, followed N-acetylation cysteine. MAbs...

Laser induced breakdown spectroscopy (LIBS) is commonly used to identify elemental compositions of various samples. To facilitate this task, we propose the use an spectral library for single-pulsed, nanosecond LIBS in range 198-968 nm. This spectroscopic generated by measuring optical emissions from plasmas 40 pure elements. demonstrate usefulness proposed database, measure and analyze spectra iron ethanol show that these samples with a high degree certainty.

Classification and identification of amino acids in aqueous solutions is important the study biomacromolecules. Laser Induced Breakdown Spectroscopy (LIBS) uses high energy laser-pulses for ablation chemical compounds whose radiated spectra are captured recorded to reveal molecular structure. Spectral peaks noise from LIBS impacted by experimental protocols. Current methods spectral analysis achieves promising results using PCA, a linear method. It well-known that underlying physical...