A5029532798- Insect and Arachnid Ecology and Behavior
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect Utilization and Effects
- Insect Pheromone Research and Control
- Genomics and Phylogenetic Studies
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Genomics and Rare Diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Studies on Chitinases and Chitosanases
- Cancer Genomics and Diagnostics
- Bioinformatics and Genomic Networks
- Heat shock proteins research
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- thermodynamics and calorimetric analyses
- Viral Infectious Diseases and Gene Expression in Insects
University of Georgia
2021-2024
Iowa State University
2019-2021
Vanderbilt University
2017-2018
Baylor College of Medicine
2018
Abstract Mosquitoes shift from detritus-feeding larvae to blood-feeding adults that can vector pathogens humans and other vertebrates. The sugar blood meals consume are rich in carbohydrates protein but deficient nutrients including B vitamins. Facultatively hematophagous insects like mosquitoes have been hypothesized avoid vitamin deficiencies by carryover of resources the larval stage. However, prior experimental studies also used with a gut microbiota could provision Here, we Aedes...
Female mosquitoes produce eggs in gonadotrophic cycles that are divided between a previtellogenic and vitellogenic phase. Previtellogenic females consume water sugar sources like nectar while also being attracted to hosts for blood feeding. Consumption of meal activates the phase, which produces mature suppresses host attraction. In this study, we tested hypothesis neuropeptide Y-like hormones differentially modulate attraction behavior mosquito Aedes aegypti . A series experiments...
Anautogenous mosquitoes must blood feed on a vertebrate host to produce eggs. Each gonadotrophic cycle is subdivided into sugar-feeding previtellogenic phase that produces primary follicles and meal-activated vitellogenic in which large numbers of eggs synchronously mature are laid. Multiple endocrine factors including juvenile hormone (JH), insulin-like peptides (ILPs), ovary ecdysteroidogenic (OEH), 20-hydroxyecdysone (20E) coordinate each cycle. Egg formation also requires nutrients from...
The pink bollworm, Pectinophora gossypiella, is a world-wide pest of cotton and in some parts the growing region controlled by mating disruption technique using synthetic sex pheromone. pheromone consists two compounds, (Z,Z)- (Z,E)-7,11-hexadecadienyl acetates, about 50:50 ratio. However, recently, population with compound ratios 62:38 were found fields that use Israel. To investigate how change developed, we compared gland transcriptomes between reference laboratory obtained from an...
In this study, we analyzed the transcriptome obtained from pheromone gland isolated two Israeli populations of pink bollworm Pectinophora gossypiella to identify viral sequences. The lab population and field samples carried same We discovered four novel viruses: positive-sense single-stranded RNA viruses, virus 1 (PecgV1, a Iflaviridae) 4 (PecgV4, unclassified), negative-sense 2 (PecgV2, Phasmaviridae) 3 (PecgV3, Phenuiviridae). addition, sequences derived viruses that belong Mononegavirales...
Macrocentrus cingulum is a principal endoparasite of Ostrinia furnacalis larvae. M. larvae repress host immune responses for survival and ingest nutrients development until emerging. However, most investigations focused on the mechanisms how wasps immunity, triggered nutrient status altered by in are neglected. In this study, we found that parasitized O. activated fast recognition produced some effectors such as lysozyme antimicrobial peptides, along with more consumption trehalose, glucose,...
Abstract Differential Scanning Fluorimetry Guided Refolding (DGR) is a simple methodology that can be used to rapidly screen for and identify conditions capable of accurately refolding protein preparations, such as those obtained from Escherichia coli inclusion bodies. It allows the production in E. functional proteins would otherwise require far more expensive methods. This unit describes how set up DGR assay, perform trials microplate format, use MeltTraceur Web software interactively...
Abstract The spatial distribution of genetic variation within proteins is shaped by evolutionary constraint and thus can provide insights into the functional importance protein regions potential pathogenicity alterations. Here, we comprehensively evaluate 3D patterns on human germline somatic in 4,568 solved structures. Different classes coding variants have significantly different distributions. Neutral missense exhibit a range patterns, with general trend dispersion driven core residues....
Matrix metalloproteinases (MMPs) are crucial for tissue remodeling and immune responses in insects, yet it remains unclear how MMPs affect the various processes against pathogenic infections whether vary among insects. In this study, we used lepidopteran pest Ostrinia furnacalis larvae to address these questions by examining changes of immune-related gene expression antimicrobial activity after knockdown MMP14 bacterial infections. We identified O. using rapid amplification complementary DNA...