A5036350876- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Cellular Mechanics and Interactions
- Erythrocyte Function and Pathophysiology
- Cardiomyopathy and Myosin Studies
- CRISPR and Genetic Engineering
- Nanopore and Nanochannel Transport Studies
- Electrohydrodynamics and Fluid Dynamics
- Micro and Nano Robotics
- Cardiovascular Effects of Exercise
- Force Microscopy Techniques and Applications
- Heat shock proteins research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Enzyme Catalysis and Immobilization
- Mesenchymal stem cell research
- RNA Research and Splicing
CFD Research Corporation (United States)
2018-2023
University of Michigan
2014-2018
Huntsville Hospital
2018
Michigan United
2016
Ann Arbor Center for Independent Living
2014
Directed evolution has long been a key strategy to generate enzymes with desired properties like high selectivity, but experimental barriers and analytical costs of screening enormous mutant libraries have limited such efforts. Here, we describe an ultrahigh-throughput dual-channel microfluidic droplet system that can be used screen up ~10
Micropipette aspiration measures the mechanical properties of single cells. A traditional micropipette system requires a bulky infrastructure and has low throughput limited potential for automation. We have developed simple microfluidic device which is able to trap apply pressure cells in designated arrays. By changing volume flow rate using syringe pump, we can accurately exert difference across trapped pipette aspiration. examining cell deformation protrusion length into under an optical...
Mutations in cardiac myosin binding protein C (MyBP-C, encoded by MYBPC3) are the most common cause of hypertrophic cardiomyopathy (HCM). Most MYBPC3 mutations result premature termination codons (PTCs) that RNA degradation and a reduction MyBP-C HCM patient hearts. However, has not been consistently observed MYBPC3-mutant induced pluripotent stem cell cardiomyocytes (iPSCMs). To determine early mutation effects, we used genome-engineered iPSCMs. iPSCMs with frameshift were compared promoter...
Human adipose tissue is a rich source of mesenchymal stem cells (MSCs). adipose-derived (ADSCs) are first prepared by digestion lipoaspirate. The remaining constituent contains mixture ADSCs, other cell types and lysed fragments. We have developed scalable microfluidic sorter cascade which enabled high-throughput label-free enrichment ADSCs from tissue-digested human samples to improve the quality purified product. continuous was composed spiral-shaped inertial deterministic lateral...
Cardiac myosin binding protein C (MYBPC3) is the most commonly mutated gene associated with hypertrophic cardiomyopathy (HCM). Haploinsufficiency of full-length MYBPC3 and disruption proteostasis have both been proposed as central to HCM disease pathogenesis. Discriminating relative contributions these 2 mechanisms requires fundamental knowledge how turnover WT mutant proteins regulated. We expressed several disease-causing mutations in primary neonatal rat ventricular cardiomyocytes. In...
Various micro-engineered tools or platforms have been developed recently for cell mechanics studies based on acoustic, magnetic, and optical actuations. Compared with other techniques single manipulations, microfluidics has the advantages simple working principles device implementations. In this work, we develop a multi-layer microfluidic pipette aspiration integrated pneumatically actuated control valves. This configuration enables decoupling of trapping aspiration, hence causes less...
Abstract All living organisms sense mechanical forces. Engineering mechanosensitive artificial cell through bottom-up in vitro reconstitution offers a way to understand how mixtures of macromolecules assemble and organize into complex system that responds We use stable double emulsion droplets (aqueous/oil/aqueous) prototype cells. In order demonstrate mechanosensation cells, we develop novel microfluidic device is capable trapping emulsions designated chambers, followed by compression...
Isolation of pure populations mesenchymal stem cells from bone marrow aspirate is a critical need in regenerative medicine such as orthopedic and cartilage reconstruction with important clinical therapeutic implications.
Metastatic cancer cells migrate through constricted spaces and experience significant compressive stress, but mechanisms enabling migration in confined geometries remain unclear. Cancer cell within 3-dimensional (3D) microfluidic channels has been shown to be distinct from 2D migration. However, whether 3D can manipulated by mechanosensory components not examined detail. In this work, we exogenously introduced a mechanosensitive channel of large conductance (MscL) into metastatic breast...
Separation of blood components is required in many diagnostic applications and processes. In laboratories, usually fractionated by manual operation involving a bulk centrifugation equipment, which significantly increases logistic burden. Blood sample processing the field resource-limited settings cannot be readily implemented without use microfluidic technology. this study, we developed small footprint, rapid, passive channel device that relied on margination inertial focusing effects for...