A5091593293- Cystic Fibrosis Research Advances
- Cell Adhesion Molecules Research
- Neonatal Respiratory Health Research
- Cellular transport and secretion
- Renal Diseases and Glomerulopathies
- Nanoplatforms for cancer theranostics
- Porphyrin and Phthalocyanine Chemistry
- Photodynamic Therapy Research Studies
- Retinoids in leukemia and cellular processes
- interferon and immune responses
- Retinal Diseases and Treatments
- Advanced biosensing and bioanalysis techniques
- Immunodeficiency and Autoimmune Disorders
- Retinopathy of Prematurity Studies
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Photoreceptor and optogenetics research
- Fullerene Chemistry and Applications
- Complement system in diseases
- Inhalation and Respiratory Drug Delivery
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Lipid metabolism and disorders
- Telomeres, Telomerase, and Senescence
Toyama Prefectural University
2017-2024
Kwansei Gakuin University
2018-2024
Kumamoto University
2009-2022
Japan Society for the Promotion of Science
2020
McGill University
2017-2019
Kyoto University
2017
Tokyo Denki University
2013
Kanazawa University
1993
The process of cell death caused by influenza virus infection in cultured MDCK and HeLa cells was analysed. This gave rise to nuclear fragmentation chromatin condensation accompanied chromosomal DNA into oligonucleosomes. Chromosomal progressed concomitantly with lysis cells, producing high low yields particles, respectively, indicating that the extent not proportional production. endonuclease inhibitor zinc blocked cells. Cycloheximide inhibited as well lysis. Inhibition occurred when drug...
Molecular chaperones are pivotal in folding and degradation of the cellular proteome but their impact on conformational dynamics near-native membrane proteins with disease relevance remains unknown. Here we report effect chaperone activity functional conformation temperature-sensitive mutant cystic fibrosis channel (∆F508-CFTR) at plasma after reconstitution into phospholipid bilayer. Thermally induced unfolding 37 °C concomitant inactivation ∆F508-CFTR partially suppressed by constitutive...
Toll-like receptor 3 (TLR3) has gained recognition as a novel molecular target for cancer therapy because TLR3 activation by its synthetic ligand poly I:C directly causes tumor cell death. Recently, we reported that suppressor p53 increases the expression of in several lines. Another study also showed interferon-alpha (IFN-alpha) up-regulates expression. We thus hypothesized various anticancer drugs such p53-activating reagents and IFNs may potentiate I:C-induced death through up-regulation...
Alport syndrome (AS) is a hereditary, progressive nephritis caused by mutation of type IV collagen. Previous studies have shown that activation signal transducer and activator transcription 3 (STAT3) exacerbates other renal diseases, but whether STAT3 AS pathology still unknown. Here we aim to investigate the involvement in progression AS.Phosphorylated expression was assessed immunoblotting analysis kidneys glomeruli an mouse model (Col4a5 G5X mutant). To determine effect blocking...
Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role in lysosomal delivery has been well documented, but its the recycling pathway largely unknown. Here, we report that ubiquitin (Ub) ligase RFFL regulates regulators. An dominant-negative (DN) mutant induced clustering compartments (ERCs) delayed cargo without affecting traffic. A BioID interactome analysis revealed interacts with Rab11 effectors EHD1, MICALL1...
The peripheral protein quality control (periQC) system eliminates the conformationally defective cystic fibrosis transmembrane conductance regulator (CFTR), including ∆F508-CFTR, from plasma membrane (PM) and limits efficacy of pharmacological therapy for (CF). ubiquitin (Ub) ligase RFFL is responsible chaperone-independent ubiquitination lysosomal degradation CFTR in periQC. Here, we report that Ub RNF34 participates periQC parallel to RFFL. An vitro study reveals directly recognizes NBD1...
Alport syndrome (AS) is one of the most common types inherited nephritis caused by mutation in glomerular basement membrane components. AS characterized proteinuria at early stage disease and hyperplastic phenotype renal fibrosis late stage. Here, we show that global deficiency tumor suppressor p53 significantly accelerated progression X-linked mice decreased lifespan these mice. protein expression was detected 21-week-old wild-type but not age-matched Expression proinflammatory cytokines...
Semiconducting single-walled carbon nanotubes (s-SWNTs) are capable of fluorescence emission as well photothermal and photodynamic actions, resulting from their near-infrared (NIR) absorptions corresponding to S11 S22 transitions. Here, we show that one chiral s-SWNTs, (6,4)-SWNTs, photogenerates all three the major reactive oxygen species, i.e., singlet (1O2), superoxide anion (O2•–), hydroxyl radical (•OH), in a sustainable manner. Its efficiency for •OH generation is dramatically higher...
Alport syndrome is a hereditary glomerulopathy with proteinuria and nephritis caused by defects in genes encoding type IV collagen the glomerular basement membrane. All male most female patients develop end-stage renal disease. Effective treatment to stop or decelerate progression of still under investigation. Here we showed that combination mild electrical stress (MES) heat (HS) ameliorated progressive injury mouse model syndrome. The expressions kidney marker neutrophil...
Abstract Two features of meso ‐Aryl‐substituted expanded porphyrins suggest suitability as theranostic agents. They have excellent absorption in near infrared (NIR) region, and they offer the possibility introduction multiple fluorine atoms at structurally equivalent positions. Here, hexaphyrin (hexa) was synthesized from 2,6‐bis(trifluoromethyl)‐4‐formyl benzoate pyrrole evaluated a novel porphyrin with above features. Under NIR illumination hexa showed intense photothermal weak...
Myeloid Elf-1-like factor (MEF) or Elf4 is an ETS transcription that activates innate immunity-associated genes such as lysozyme (LYZ), human β-defensin 2 (HβD2), and interleukin-8 (IL-8) in epithelial cells also known to influence cell cycle progression. MEF transcriptionally activated by E2F1, but the E2F1-mediated transcriptional activation inhibited p53 through E2F1-p53 protein interaction. Although of has been investigated depth, its post-translational regulation not well explored. By...
Myeloid elf-1-like factor (MEF) or Elf4 is an E-twenty-six (ETS)-related transcription with strong transcriptional activity that influences cellular senescence by affecting tumor suppressor p53. MEF downregulates p53 expression and inhibits p53-mediated transcriptionally activating MDM2. However, whether reciprocally opposes remains unex-plored. Here, we show modulated in human cells mice tissues. promoter were suppressed While found does not contain response elements, intriguingly, it...
COPD is a lifestyle-related disease resulting from irreversible damage to respiratory tissues mostly due chronic exposure environmental pollutants, including cigarette smoke. Environmental pathogens and pollutants induce the acquired dysfunction of CFTR Cl- channel, which invoked in COPD. Despite increased incidence polymorphism R75Q or M470V patients, mechanism how variant affects pathogenesis remains unclear. Here, we investigated impact polymorphisms (R75Q, M470V) on function airway...
The ubiquitin E3 ligase UBE3C promotes the proteasomal degradation of cytosolic proteins and endoplasmic reticulum (ER) membrane proteins. is proposed to function downstream RNF185/MBRL ER-associated (ERAD) branch, contributing ERAD select Here, we report that facilitates misfolded CFTR, even in absence both RNF185 its functional ortholog RNF5 (RNF5/185). Unlike RNF5/185, had a limited impact on ubiquitination CFTR. knockdown (KD) resulted an additional increase ∆F508-CFTR channels plasma...
ABSTRACT Apical polarity of cystic fibrosis transmembrane conductance regulator (CFTR) is essential for solute and water transport in secretory epithelia can be impaired human diseases. Maintenance apical the face CFTR non-polarized delivery inefficient retention mutant CFTRs lacking PDZ-domain protein (NHERF1, also known as SLC9A3R1) interaction, remains enigmatic. Here, we show that basolateral originates from biosynthetic (∼35%) endocytic (∼65%) recycling missorting. Basolateral channels...