A5001693489- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fluorescence Microscopy Techniques
- Advanced Fiber Optic Sensors
- Cell Image Analysis Techniques
- Photonic and Optical Devices
- RNA Interference and Gene Delivery
- Photoreceptor and optogenetics research
- Mass Spectrometry Techniques and Applications
- Lipid Membrane Structure and Behavior
- Mechanical and Optical Resonators
- RNA Research and Splicing
- Microfluidic and Bio-sensing Technologies
- Advanced Electron Microscopy Techniques and Applications
- Ion-surface interactions and analysis
- RNA modifications and cancer
- Nuclear Structure and Function
- Semiconductor Lasers and Optical Devices
Chalmers University of Technology
2020-2024
London Centre for Nanotechnology
2019
King's College London
2019
Institut de Biosciences et Biotechnologies
2018
Université Grenoble Alpes
2014-2017
Institut pour l'avancée des biosciences
2014-2017
Inserm
2014-2017
Centre National de la Recherche Scientifique
2017
Centre Hospitalier Universitaire de Grenoble
2014
École Polytechnique Fédérale de Lausanne
2013
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in lateral direction, and 30-50 axial direction. However, there important requirement perform this type imaging: making dye molecules blink. This usually relies on utilization complex buffers, containing different chemicals sensitive enzymatic systems, limiting reproducibility method. We report here that commercial mounting medium Vectashield can be used Alexa-647, yields images comparable or...
Cancer metastasis is a complex process involving cell-cell interactions mediated by cell adhesive molecules. In this study we determine the adhesion strength between an endothelial monolayer and tumor cells of different metastatic potentials using Atomic Force Microscopy. We show that rupture forces receptor-ligand bonds increase with retraction speed range 20 70 pN. It shown most invasive lines (T24, J82) form strongest cells. Using ICAM-1 coated substrates monoclonal antibody specific for...
Abstract With the central role of nucleic acids there is a need for development fluorophores that facilitate visualization processes involving without perturbing their natural properties and behaviour. Here, we incorporate new analogue adenine, 2CNqA, into both DNA RNA, evaluate its nucleobase-mimicking internal fluorophore capacities. We find 2CNqA displays excellent photophysical in acids, highly specific thymine/uracil, maintains slightly stabilises canonical conformations RNA duplexes....
It is widely appreciated that double stranded DNA (dsDNA) subjected to strong and dynamic mechanical forces in cells. Under increasing tension B-DNA, the most stable double-stranded (ds) form of DNA, undergoes cooperative elongation into a mixture S-DNA single (ssDNA). Despite significant effort, structure, energetics, kinetics biological role remains obscure. We here stretch 60 base pair (bp) dsDNA oligonucleotides with variable number tricyclic cytosine, tC, modifications using optical...
We characterize the stability, conformational dynamics, (un)folding pathways, as well identify a promising drug–target site within SL4 hairpin of SARS-CoV-2 by combining base analogues and optical tweezers.
Investigating the forces in nucleic acids using single base-pair level modifications and optical tweezers.
Pre-miRNA-377 is a hairpin-shaped regulatory RNA associated with heart failure. Here, we use single-molecule optical tweezers to unzip pre-miRNA-377 and study its stability dynamics. We show that magnesium ions have strong stabilizing effect, sodium stabilize the hairpin more than potassium ions. The unfolds in single step, regardless of buffer composition. Interestingly, folding occurs either step (type 1) or through formation intermediates, multiple steps 2) gradually 3). Type 3 only...