A5101979629- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Viral gastroenteritis research and epidemiology
- Protein Degradation and Inhibitors
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Cell Adhesion Molecules Research
- Galectins and Cancer Biology
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Ferroptosis and cancer prognosis
- Adenosine and Purinergic Signaling
- Rabies epidemiology and control
- NF-κB Signaling Pathways
Zhejiang Chinese Medical University
2019-2025
Duke-NUS Medical School
2025
National University of Singapore
2025
University of South China
2021-2024
Southwest Minzu University
2018-2024
Shanghai University of Traditional Chinese Medicine
2024
Anhui Medical University
2013-2022
First Affiliated Hospital of Anhui Medical University
2022
Shenzhen Second People's Hospital
2018-2020
CAE (Canada)
2020
We have previously reported the selective amplification of several rat striatal cDNA sequences that encode guanine nucleotide-binding regulatory protein (G protein)-coupled receptors. One these (R226) exhibited high sequence identity (58%) with two cloned adenosine A full-length clone for R226 has been isolated from a brain library. The encodes 320 amino acids can be organized into seven transmembrane stretches. expressed in COS-7 and CHO cells membranes transfected were screened receptor...
Triple-negative (ER(-), HER2(-), PR(-)) breast cancer (TNBC) is an aggressive disease with a poor prognosis no available molecularly targeted therapy. Silencing of microRNA-145 (miR-145) may be defining marker TNBC based on molecular profiling and deep sequencing. Therefore, the mechanism behind miR-145 downregulation in was examined. Overexpression long intergenic noncoding RNA regulator reprogramming, lincRNA-RoR, functions as competitive endogenous sponge TNBC. Interestingly, lincRNA-RoR...
Three genes closely related to the D1 dopamine receptor were identified in human genome. One of lacks introns and encodes a functional receptor, D5, whose deduced amino acid sequence is 49% identical that receptor. Compared with D5 displayed higher affinity for was able stimulate biphasic rather than monophasic intracellular accumulation cAMP. Neither other two direct synthesis Nucleotide analysis revealed these are 98% each 95% sequence. Relative sequence, both contain insertions deletions...
Abstract Histone deacetylases (HDAC) play a critical role in chromatin modification and gene expression. Recent evidence indicates that HDACs can also regulate functions of nonhistone proteins by catalyzing the removal acetylated lysine residues. Here, we show HDAC inhibitor LBH589 down-regulates DNA methyltransferase 1 (DNMT1) protein expression nucleus human breast cancer cells. Cotreatment with proteasomal MG-132 abolishes ability to reduce DNMT1, suggesting pathway mediates DNMT1...
Our previous studies demonstrated that inhibition of histone deacetylases (HDACs) by trichostatin A reactivates estrogen receptor alpha (ER) gene expression in ER-negative breast cancer cells. Here, we use the clinically relevant HDAC inhibitor, LBH589 (LBH) to explore roles ER silencing. In human lines, MDA-MB-231 and MDA-MB-435, treatment with LBH for 24 hours restored mRNA protein without a concomitant demethylation CpG island at promoter. The was sustained least 96 after withdrawal...
More than 40% of the U.S. population are clinically obese and suffer from metabolic syndrome with an increased risk postmenopausal estrogen receptor-positive breast cancer. Adipocytes primary component adipose tissue formed through adipogenesis precursor mesenchymal stem cells. While major molecular pathways understood, little is known about noncoding RNA signaling networks involved in adipogenesis. Using adipocyte-derived cells (ADSCs) isolated wild-type microRNA 140 (miR-140) knockout...
// Pang-Kuo Lo 1 , Yongshu Zhang Benjamin Wolfson Ramkishore Gernapudi Yuan Yao Nadire Duru Qun Zhou Department of Biochemistry and Molecular Biology, Greenebaum Cancer Center, University Maryland School Medicine, Baltimore, MD 21201, USA Correspondence to: Zhou, email: qzhou@som.umaryland.edu Keywords: BRCA1, NEAT1, miR-129, WNT4, breast cancer stem cells Received: March 18, 2016 Accepted: August 10, Published: 2016 ABSTRACT Dysregulation long non-codng RNA...
E2F is a group of genes that encode family transcription factors (TFs) in higher eukaryotes and participate cell cycle regulation DNA synthesis mammalian cells. Evidence from lines, mouse models, human tissues indicates TFs are implicated lung cancer (LC) tumorigenesis. However, the diverse expression patterns prognostic values eight E2Fs have yet to be elucidated. In current study, we examined transcriptional survival data patients with LC ONCOMINE, GEPIA, Kaplan-Meier Plotter, cBioPortal...
Quinidine inhibits proliferation and promotes cellular differentiation in human breast tumor epithelial cells. Previously we showed quinidine arrested MCF-7 cells G<sub>1</sub> phase of the cell cycle led to a G<sub>0</sub> transition followed by apoptotic death. The present experiments demonstrated that MCF-7, MCF-7<i>ras</i>, T47D, MDA-MB-231, MDA-MB-435 transiently differentiate before undergoing apoptosis response quinidine. accumulated lipid droplets, cytokeratin 18 cytoskeleton was...
Acute kidney injury (AKI) is a common clinical syndrome worldwide, with no effective treatment strategy. Renal ischemia-reperfusion (IR) one of the main AKI features, and excessive reactive oxygen species (ROS) production during reperfusion causes severe oxidative damage to kidney. Loureirin C (LC), an active ingredient in traditional Chinese medicine dragon's blood, possesses excellent antioxidative properties, but its role renal IR not clear. In this study, we evaluated protective effects...
Long noncoding RNAs (lncRNAs) have emerged as key regulators of gene expression in embryonic stem cell (ESC) self-renewal and differentiation. In ESCs, lncRNAs are regulated at the genetic level via transcription factor binding to lncRNA promoters. Here we demonstrate that cytoprotective NRF2 controls mammary cells. By profiling wild-type knockdown cells, ROR, a regulator pluripotency, is overexpressed upon knockdown. We performed promoter analyses examined predicted elements ROR using...
20S-protopanaxadiol (aPPD) is a major gastrointestinal metabolic product of ginsenosides. The latter share structural similarity with steroids and are the main pharmacologically active component in ginseng.The authors investigated interaction between aPPD estrogen receptors (ER) human breast adenocarcinoma MCF-7 cells through receptor binding assay, ER-induced gene expression, cell proliferation both vitro vivo.aPPD, but not its close analog ginsenosides, competed [(3)H]-17-beta estradiol...
ABSTRACT The molecular mechanisms of signal transduction plants in response to infection by Verticillium dahliae (VD) are not well understood. We previously showed that NO may act as an upstream signalling molecule trigger the depolymerization cortical microtubules Arabidopsis . In present study, we used wild‐type, and atrbohD atrbohF mutants explore action H 2 O signals dynamic microtubule cytoskeleton defence responses. demonstrated also regulate depolymerization. played a functional role...