Berberine is widely used in traditional Iranian medicine to treat diabetes and inflammatory conditions. This study was aimed at developing a method for the preparation of nanoparticles (Nano-Ber) order improve its aqueous-phase solubility complex formation with human serum albumin (HSA) holo-transferrin (HTF) from viewpoint interaction behavior. Nano-Ber prepared olive oil as phase, Tween 80 surfactant Span 60 co-surfactant. obtained spherical shape mean particle size 43.7 ± 3.6 nm, an...

As a waste material, the amazing potential of cellulose nanocrystals (CNCs) isolated from Citrus medica L. pericarp in being natural resource lingo-cellulosic products has never been investigated before. In present study, an alkaline pretreatment and two-step bleaching procedure were applied to conduct desired acid hydrolysis by usage 64% sulfuric at 50°C for 105 min. The extracted CNCs distinguished through means transmission electron microscopy (TEM), field emission scanning (FESEM), X-ray...

In this study, we determined the interaction of piperine and calf thymus DNA (ct DNA) in Tris-HCl buffer solution at pH = 6.8 also evaluated binding mechanism through data multi-spectroscopic techniques along with thermal melting viscosity measurements. The outcomes fluorescence quenching confirmed occurrence interactions between ctDNA pointed out role as quencher. addition, K

Abstract In this study, cellulose nanocrystals (CNCs) were synthesized from celery stalks to be used as the platform for quercetin delivery. Additionally, CNCs and CNCs–quercetin characterized using results of scanning electron microscope (SEM), transmission microscopy (TEM), X‐ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, zeta potential, while their interactions with human holo‐transferrin (HTF) also investigated. We examined interaction under physiological...

Hen egg white lysozyme (HEWL) was hydrolyzed with papain, trypsin and a combination of the two to isolate antioxidant peptides. The prepared hydrolysates were evaluated for activity using DPPH ABTS radical scavenging, metal ion chelation lipid peroxidation inhibition. obtained hydrolysate by enzymes exhibited highest compared other elected isolation peptides reverse-phase high-performance liquid chromatography (RP-HPLC). A most potent fraction namely F2 fraction, identified be...

AbstractThe interaction between metformin and human serum albumin (HSA), as well its glycated form (gHSA) was investigated by multiple spectroscopic techniques, zeta potential, molecular modeling under physiological conditions. The steady state time-resolved fluorescence data displayed the quenching mechanism of HSA–metformin gHSA–metformin static. binding information, including constants, number sites, effective constant showed that affinity to HSA greater than gHSA which also confirmed...

The binding of ciprofloxacin to human serum albumin (HSA) in the presence and absence silver nanoparticles three sizes was investigated for first time. For this purpose fluorescence spectroscopy, circular dichroism, UV–vis ζ potential techniques were employed under physiological conditions. titration results indicated that quenched intensity HSA through a static mechanism, but Ag I II there two different kinds interaction behavior. Results dichroism secondary structure modified with...

In this study, we have investigated the effects of Nano-curcumin (Nano-CUR) binding on HSA-HTF interactions as binary and ternary systems, which had been done through multiple spectroscopic MD simulation. It has indicated by fluorescence spectroscopy that Nano-CUR is capable quenching both proteins with a static mechanism. Thermodynamic parameters calculated considering data at different temperatures. The constants HSA-Nano-CUR, HTF-Nano-CUR (HSA-HTF) complexes formation were (2.03 ±...

This article describes, for the first time, effect of three different sizes silver nanoparticles on binding curcumin to lysozyme as examined by spectroscopic and zeta potential techniques at physiological conditions. The constants in presence were measured. Based results synchronous fluorescence three-dimensional spectroscopy, caused conformational changes during curcumin. Such also observed when increasing concentration. resonance energy transfer theory indicated that could change distance...

The present study was carried out to characterize Angiotensin-converting enzyme (ACE) inhibitory peptides which are released from the trypsin hydrolysate of wheat gluten protein. binding two peptide (P4 and P6) human serum albumin (HSA) under physiological conditions has been investigated by multi-spectroscopic in combination with molecular modeling techniques. Time-resolved quenching fluorescence spectroscopies results revealed that HSA P4 P6 binary ternary systems caused HSA-peptides...

DNA is the primary target of many anticancer drugs involved in important intercellular processes, especially transcriptional regulation, and histone known to inhibit gene expression. Small molecules can bind histone-DNA impair cell division, growth, inhibition, apoptosis cancer cells. In this research, interaction a H1-calf thymus (ct DNA) complex propyl acridone (PA) was investigated Tris-HCl buffer, pH 6.8, using multi-spectroscopic, viscosity, molecular modeling techniques. The Stern...

With advances in new drug therapies, it is essential to understand the interactions between drugs and target molecules. In this study, we applied multiple spectroscopic techniques including absorbance, fluorescence, circular dichroism spectroscopy, viscosity, thermal melting, calorimetric, molecular dynamics (MD) simulation study interaction 2-Ethyl-5-(4-methylphenyl) pyramido pyrazole ophthalazine trione (PPF) calf thymus DNA (ct DNA) absence or presence of histone H1. PPF exhibits a high...

The present study was designed to investigate the influence of two indispensable and dispensable amino acids, including methionine, histidine, cysteine proline, on binding interaction between human serum albumin (HSA) an antibiotic agent lomefloxacin (LMF). fluorescence quenching experiments showed that intrinsic emission HSA considerably quenched following LMF in all systems. Furthermore, interactions maximum wavelength slightly decreased. spectral changes observed systems we e attributed...

Demonstrates the overlap that had been induced between fluorescence emission spectrum of Hb and absorption drugs, which has proved there is a high probability to occurrence energy transfer from LMF in absence presence NRF.